Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4372 and GFF4373 are separated by 224 nucleotides GFF4373 and GFF4374 are separated by 11 nucleotides GFF4374 and GFF4375 are separated by 55 nucleotides GFF4375 and GFF4376 are separated by 116 nucleotides
GFF4372 - Uncharacterized protein ylaB, at 154,791 to 156,341
GFF4372
GFF4373 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-independent, at 156,566 to 156,826
GFF4373
GFF4374 - LSU ribosomal protein L36p, at 156,838 to 156,972
GFF4374
GFF4375 - Putative inner membrane protein, at 157,028 to 157,498
GFF4375
GFF4376 - Maltose O-acetyltransferase (EC 2.3.1.79), at 157,615 to 158,166
GFF4376
Position (kb)
156
157 Strain fitness (log2 ratio)
-1
0
1
2 at 155.887 kb on + strand, within GFF4372 at 155.888 kb on - strand, within GFF4372 at 156.003 kb on - strand, within GFF4372 at 156.188 kb on + strand at 156.193 kb on - strand at 156.193 kb on - strand at 156.354 kb on - strand at 156.376 kb on - strand at 156.455 kb on + strand at 156.589 kb on - strand at 156.603 kb on + strand, within GFF4373 at 156.629 kb on + strand, within GFF4373 at 156.629 kb on + strand, within GFF4373 at 156.629 kb on + strand, within GFF4373 at 156.630 kb on - strand, within GFF4373 at 156.734 kb on + strand, within GFF4373 at 156.908 kb on + strand, within GFF4374 at 157.069 kb on - strand at 157.069 kb on - strand at 157.204 kb on - strand, within GFF4375 at 157.238 kb on - strand, within GFF4375 at 157.240 kb on - strand, within GFF4375 at 157.280 kb on - strand, within GFF4375 at 157.371 kb on + strand, within GFF4375 at 157.372 kb on - strand, within GFF4375 at 157.372 kb on - strand, within GFF4375 at 157.734 kb on + strand, within GFF4376 at 157.789 kb on + strand, within GFF4376 at 157.842 kb on - strand, within GFF4376 at 157.842 kb on - strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.845 kb on + strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.846 kb on - strand, within GFF4376 at 157.850 kb on - strand, within GFF4376
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 155,887 + GFF4372 0.71 +0.5 155,888 - GFF4372 0.71 +0.2 156,003 - GFF4372 0.78 +2.3 156,188 + -0.7 156,193 - +1.1 156,193 - +0.7 156,354 - -1.1 156,376 - -1.1 156,455 + +0.4 156,589 - +0.4 156,603 + GFF4373 0.14 +0.3 156,629 + GFF4373 0.24 +0.7 156,629 + GFF4373 0.24 +1.5 156,629 + GFF4373 0.24 +0.0 156,630 - GFF4373 0.25 -0.3 156,734 + GFF4373 0.64 -1.7 156,908 + GFF4374 0.52 +0.5 157,069 - -0.8 157,069 - -0.2 157,204 - GFF4375 0.37 +0.0 157,238 - GFF4375 0.45 -0.1 157,240 - GFF4375 0.45 -0.2 157,280 - GFF4375 0.54 -0.8 157,371 + GFF4375 0.73 +0.3 157,372 - GFF4375 0.73 -0.1 157,372 - GFF4375 0.73 -0.2 157,734 + GFF4376 0.22 +2.4 157,789 + GFF4376 0.32 +0.2 157,842 - GFF4376 0.41 -0.3 157,842 - GFF4376 0.41 -0.3 157,845 + GFF4376 0.42 +0.8 157,845 + GFF4376 0.42 +0.4 157,845 + GFF4376 0.42 +0.0 157,845 + GFF4376 0.42 +0.5 157,845 + GFF4376 0.42 +1.9 157,846 - GFF4376 0.42 +1.3 157,846 - GFF4376 0.42 -0.0 157,846 - GFF4376 0.42 -0.0 157,846 - GFF4376 0.42 +0.7 157,846 - GFF4376 0.42 -0.2 157,846 - GFF4376 0.42 +0.0 157,846 - GFF4376 0.42 +0.0 157,846 - GFF4376 0.42 +0.0 157,846 - GFF4376 0.42 -0.0 157,846 - GFF4376 0.42 +1.2 157,850 - GFF4376 0.43 -1.3
Or see this region's nucleotide sequence