Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4163 and GFF4164 are separated by 122 nucleotides GFF4164 and GFF4165 are separated by 34 nucleotides GFF4165 and GFF4166 are separated by 84 nucleotides
GFF4163 - NAD kinase (EC 2.7.1.23), at 54,319 to 55,197
GFF4163
GFF4164 - Heat shock protein GrpE, at 55,320 to 55,910
GFF4164
GFF4165 - FIG074102: hypothetical protein, at 55,945 to 56,586
GFF4165
GFF4166 - Hemolysins and related proteins containing CBS domains, at 56,671 to 57,912
GFF4166
Position (kb) 55
56
57 Strain fitness (log2 ratio) -2
-1
0
1
2 at 55.909 kb on - strand at 56.032 kb on - strand, within GFF4165 at 56.033 kb on + strand, within GFF4165 at 56.295 kb on + strand, within GFF4165 at 56.533 kb on + strand at 56.538 kb on - strand at 56.602 kb on + strand at 56.602 kb on + strand at 56.731 kb on - strand at 56.751 kb on + strand at 56.755 kb on + strand at 56.755 kb on + strand at 56.756 kb on - strand at 56.907 kb on + strand, within GFF4166 at 56.998 kb on - strand, within GFF4166 at 57.119 kb on + strand, within GFF4166 at 57.119 kb on + strand, within GFF4166 at 57.207 kb on + strand, within GFF4166 at 57.384 kb on + strand, within GFF4166 at 57.384 kb on + strand, within GFF4166 at 57.436 kb on + strand, within GFF4166 at 57.436 kb on + strand, within GFF4166 at 57.437 kb on - strand, within GFF4166 at 57.519 kb on + strand, within GFF4166 at 57.519 kb on + strand, within GFF4166 at 57.523 kb on + strand, within GFF4166 at 57.523 kb on + strand, within GFF4166 at 57.582 kb on - strand, within GFF4166
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 55,909 - -1.0 56,032 - GFF4165 0.14 +0.1 56,033 + GFF4165 0.14 -0.7 56,295 + GFF4165 0.55 -0.1 56,533 + -2.0 56,538 - +0.2 56,602 + +0.2 56,602 + +0.3 56,731 - -0.2 56,751 + -0.6 56,755 + +2.2 56,755 + +0.1 56,756 - +0.0 56,907 + GFF4166 0.19 +0.7 56,998 - GFF4166 0.26 -1.2 57,119 + GFF4166 0.36 -0.9 57,119 + GFF4166 0.36 -0.2 57,207 + GFF4166 0.43 -1.2 57,384 + GFF4166 0.57 -0.5 57,384 + GFF4166 0.57 -0.3 57,436 + GFF4166 0.62 -0.2 57,436 + GFF4166 0.62 -0.9 57,437 - GFF4166 0.62 -0.1 57,519 + GFF4166 0.68 -0.1 57,519 + GFF4166 0.68 +0.7 57,523 + GFF4166 0.69 +0.2 57,523 + GFF4166 0.69 -0.6 57,582 - GFF4166 0.73 +1.0
Or see this region's nucleotide sequence