Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4102 and GFF4103 overlap by 30 nucleotides GFF4103 and GFF4104 are separated by 249 nucleotides GFF4104 and GFF4105 are separated by 218 nucleotides
GFF4102 - Predicted glutamine amidotransferase, at 61,482 to 62,249
GFF4102
GFF4103 - probable exported protein STY0357, at 62,220 to 62,960
GFF4103
GFF4104 - DNA polymerase IV (EC 2.7.7.7), at 63,210 to 64,265
GFF4104
GFF4105 - Protein with similarity to RtcB, at 64,484 to 65,458
GFF4105
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1 at 62.241 kb on - strand at 62.299 kb on + strand, within GFF4103 at 62.300 kb on - strand, within GFF4103 at 62.351 kb on - strand, within GFF4103 at 62.368 kb on - strand, within GFF4103 at 62.389 kb on + strand, within GFF4103 at 62.393 kb on - strand, within GFF4103 at 62.402 kb on + strand, within GFF4103 at 62.530 kb on + strand, within GFF4103 at 62.570 kb on - strand, within GFF4103 at 62.630 kb on + strand, within GFF4103 at 62.693 kb on + strand, within GFF4103 at 62.768 kb on - strand, within GFF4103 at 62.856 kb on - strand, within GFF4103 at 62.977 kb on + strand at 63.120 kb on - strand at 63.120 kb on - strand at 63.129 kb on + strand at 63.130 kb on - strand at 63.223 kb on + strand at 63.234 kb on - strand at 63.287 kb on + strand at 63.331 kb on + strand, within GFF4104 at 63.367 kb on + strand, within GFF4104 at 63.368 kb on - strand, within GFF4104 at 63.368 kb on - strand, within GFF4104 at 63.371 kb on + strand, within GFF4104 at 63.371 kb on + strand, within GFF4104 at 63.397 kb on + strand, within GFF4104 at 63.444 kb on + strand, within GFF4104 at 63.467 kb on - strand, within GFF4104 at 63.467 kb on - strand, within GFF4104 at 63.694 kb on - strand, within GFF4104 at 63.912 kb on - strand, within GFF4104 at 64.291 kb on - strand at 64.572 kb on + strand at 64.576 kb on + strand at 64.576 kb on + strand at 64.751 kb on - strand, within GFF4105 at 65.219 kb on - strand, within GFF4105 at 65.251 kb on + strand, within GFF4105
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 62,241 - -0.8 62,299 + GFF4103 0.11 -0.3 62,300 - GFF4103 0.11 +0.4 62,351 - GFF4103 0.18 -0.4 62,368 - GFF4103 0.20 -0.5 62,389 + GFF4103 0.23 -0.9 62,393 - GFF4103 0.23 -0.4 62,402 + GFF4103 0.25 -0.8 62,530 + GFF4103 0.42 -0.3 62,570 - GFF4103 0.47 +0.5 62,630 + GFF4103 0.55 +0.2 62,693 + GFF4103 0.64 +0.4 62,768 - GFF4103 0.74 -0.6 62,856 - GFF4103 0.86 +0.4 62,977 + +0.0 63,120 - -0.0 63,120 - +0.1 63,129 + -1.1 63,130 - -0.6 63,223 + -0.7 63,234 - +0.1 63,287 + +0.2 63,331 + GFF4104 0.11 -1.0 63,367 + GFF4104 0.15 +0.2 63,368 - GFF4104 0.15 +0.0 63,368 - GFF4104 0.15 +0.1 63,371 + GFF4104 0.15 +0.0 63,371 + GFF4104 0.15 -0.2 63,397 + GFF4104 0.18 -0.4 63,444 + GFF4104 0.22 +0.9 63,467 - GFF4104 0.24 -1.6 63,467 - GFF4104 0.24 -0.8 63,694 - GFF4104 0.46 +1.1 63,912 - GFF4104 0.66 +0.0 64,291 - +0.4 64,572 + -1.8 64,576 + -0.4 64,576 + +0.4 64,751 - GFF4105 0.27 -0.3 65,219 - GFF4105 0.75 -0.9 65,251 + GFF4105 0.79 +1.1
Or see this region's nucleotide sequence