Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3998 and GFF3999 are separated by 175 nucleotides GFF3999 and GFF4000 are separated by 127 nucleotides GFF4000 and GFF4001 are separated by 164 nucleotides
GFF3998 - Permease of the drug/metabolite transporter (DMT) superfamily, at 270,964 to 271,884
GFF3998
GFF3999 - Putative inner membrane protein, at 272,060 to 272,971
GFF3999
GFF4000 - FIG00638072: hypothetical protein, at 273,099 to 273,233
GFF4000
GFF4001 - FIG00638997: hypothetical protein, at 273,398 to 275,110
GFF4001
Position (kb) 272
273 Strain fitness (log2 ratio) -2
-1
0
1
2 at 271.194 kb on + strand, within GFF3998 at 271.194 kb on + strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.216 kb on - strand, within GFF3998 at 271.414 kb on + strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.545 kb on + strand, within GFF3998 at 271.557 kb on + strand, within GFF3998 at 271.557 kb on + strand, within GFF3998 at 271.658 kb on - strand, within GFF3998 at 271.658 kb on - strand, within GFF3998 at 271.835 kb on + strand at 271.835 kb on + strand at 271.865 kb on - strand at 271.868 kb on - strand at 272.022 kb on + strand at 272.022 kb on + strand at 272.022 kb on + strand at 272.026 kb on + strand at 272.027 kb on - strand at 272.027 kb on - strand at 272.085 kb on + strand at 272.116 kb on + strand at 272.256 kb on + strand, within GFF3999 at 272.383 kb on + strand, within GFF3999 at 272.384 kb on - strand, within GFF3999 at 272.384 kb on - strand, within GFF3999 at 272.484 kb on + strand, within GFF3999 at 272.621 kb on + strand, within GFF3999 at 272.635 kb on + strand, within GFF3999 at 272.639 kb on - strand, within GFF3999 at 272.817 kb on + strand, within GFF3999 at 272.905 kb on + strand at 272.977 kb on + strand at 273.047 kb on + strand at 273.048 kb on - strand at 273.310 kb on + strand at 273.328 kb on + strand at 273.517 kb on + strand at 273.517 kb on + strand at 273.537 kb on + strand at 273.552 kb on - strand at 273.649 kb on + strand, within GFF4001 at 273.649 kb on + strand, within GFF4001 at 273.924 kb on - strand, within GFF4001 at 273.924 kb on - strand, within GFF4001
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 271,194 + GFF3998 0.25 -0.5 271,194 + GFF3998 0.25 +1.9 271,195 - GFF3998 0.25 +0.3 271,195 - GFF3998 0.25 +0.4 271,195 - GFF3998 0.25 +0.5 271,216 - GFF3998 0.27 +0.7 271,414 + GFF3998 0.49 -1.2 271,451 - GFF3998 0.53 +0.5 271,451 - GFF3998 0.53 +1.0 271,451 - GFF3998 0.53 +0.5 271,545 + GFF3998 0.63 +0.9 271,557 + GFF3998 0.64 +0.7 271,557 + GFF3998 0.64 +0.3 271,658 - GFF3998 0.75 -1.8 271,658 - GFF3998 0.75 -0.4 271,835 + -0.1 271,835 + +0.1 271,865 - -0.2 271,868 - -1.2 272,022 + +0.9 272,022 + -0.9 272,022 + +0.3 272,026 + -1.1 272,027 - -0.6 272,027 - -1.1 272,085 + +0.5 272,116 + -0.4 272,256 + GFF3999 0.21 +0.4 272,383 + GFF3999 0.35 -0.5 272,384 - GFF3999 0.36 -0.0 272,384 - GFF3999 0.36 +0.1 272,484 + GFF3999 0.46 +0.2 272,621 + GFF3999 0.62 +0.6 272,635 + GFF3999 0.63 +0.4 272,639 - GFF3999 0.63 -0.9 272,817 + GFF3999 0.83 +0.3 272,905 + -0.0 272,977 + +0.5 273,047 + -0.2 273,048 - +0.4 273,310 + -0.4 273,328 + -0.2 273,517 + +1.3 273,517 + +0.9 273,537 + +0.2 273,552 - -0.3 273,649 + GFF4001 0.15 +0.6 273,649 + GFF4001 0.15 +0.8 273,924 - GFF4001 0.31 -0.3 273,924 - GFF4001 0.31 -0.7
Or see this region's nucleotide sequence