Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3967 and GFF3968 overlap by 4 nucleotides GFF3968 and GFF3969 are separated by 26 nucleotides GFF3969 and GFF3970 are separated by 77 nucleotides
GFF3967 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 245,168 to 245,713
GFF3967
GFF3968 - Cobalamin synthase (EC 2.7.8.26), at 245,710 to 246,453
GFF3968
GFF3969 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 246,480 to 247,550
GFF3969
GFF3970 - L,D-transpeptidase YbiS, at 247,628 to 248,557
GFF3970
Position (kb) 246
247
248 Strain fitness (log2 ratio) -1
0
1 at 245.559 kb on + strand, within GFF3967 at 245.849 kb on + strand, within GFF3968 at 246.182 kb on - strand, within GFF3968 at 246.182 kb on - strand, within GFF3968 at 246.222 kb on - strand, within GFF3968 at 246.259 kb on - strand, within GFF3968 at 246.303 kb on + strand, within GFF3968 at 246.366 kb on + strand, within GFF3968 at 246.450 kb on - strand at 246.479 kb on + strand at 246.479 kb on + strand at 246.480 kb on - strand at 246.490 kb on + strand at 246.554 kb on + strand at 246.635 kb on + strand, within GFF3969 at 246.853 kb on + strand, within GFF3969 at 246.854 kb on - strand, within GFF3969 at 246.858 kb on - strand, within GFF3969 at 246.860 kb on + strand, within GFF3969 at 246.861 kb on - strand, within GFF3969 at 247.253 kb on - strand, within GFF3969 at 247.402 kb on - strand, within GFF3969 at 247.424 kb on - strand, within GFF3969 at 247.514 kb on + strand at 247.514 kb on + strand at 247.625 kb on - strand at 247.626 kb on + strand at 247.692 kb on + strand at 247.692 kb on + strand at 247.693 kb on - strand at 247.776 kb on + strand, within GFF3970 at 247.817 kb on - strand, within GFF3970 at 247.919 kb on - strand, within GFF3970 at 247.949 kb on + strand, within GFF3970 at 247.986 kb on + strand, within GFF3970 at 247.990 kb on + strand, within GFF3970 at 248.105 kb on - strand, within GFF3970 at 248.173 kb on - strand, within GFF3970 at 248.173 kb on - strand, within GFF3970 at 248.174 kb on + strand, within GFF3970 at 248.174 kb on + strand, within GFF3970 at 248.175 kb on - strand, within GFF3970 at 248.176 kb on + strand, within GFF3970 at 248.188 kb on - strand, within GFF3970 at 248.190 kb on - strand, within GFF3970 at 248.249 kb on - strand, within GFF3970 at 248.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 245,559 + GFF3967 0.72 +0.3 245,849 + GFF3968 0.19 +0.2 246,182 - GFF3968 0.63 +0.4 246,182 - GFF3968 0.63 +0.7 246,222 - GFF3968 0.69 -0.2 246,259 - GFF3968 0.74 +0.4 246,303 + GFF3968 0.80 -1.1 246,366 + GFF3968 0.88 +0.1 246,450 - +0.5 246,479 + +0.4 246,479 + +0.4 246,480 - +0.4 246,490 + -0.3 246,554 + +0.3 246,635 + GFF3969 0.14 -0.5 246,853 + GFF3969 0.35 +0.5 246,854 - GFF3969 0.35 -0.6 246,858 - GFF3969 0.35 -0.4 246,860 + GFF3969 0.35 -1.0 246,861 - GFF3969 0.36 -1.4 247,253 - GFF3969 0.72 -0.6 247,402 - GFF3969 0.86 -1.3 247,424 - GFF3969 0.88 -0.6 247,514 + -1.0 247,514 + -0.3 247,625 - +0.9 247,626 + +0.3 247,692 + -0.5 247,692 + +0.0 247,693 - -0.9 247,776 + GFF3970 0.16 -1.3 247,817 - GFF3970 0.20 -0.4 247,919 - GFF3970 0.31 +1.6 247,949 + GFF3970 0.35 -0.2 247,986 + GFF3970 0.38 +0.7 247,990 + GFF3970 0.39 +0.8 248,105 - GFF3970 0.51 -1.6 248,173 - GFF3970 0.59 +0.2 248,173 - GFF3970 0.59 +0.5 248,174 + GFF3970 0.59 +0.2 248,174 + GFF3970 0.59 +0.5 248,175 - GFF3970 0.59 -0.1 248,176 + GFF3970 0.59 +0.1 248,188 - GFF3970 0.60 -0.4 248,190 - GFF3970 0.60 -1.0 248,249 - GFF3970 0.67 +0.7 248,479 + +0.4
Or see this region's nucleotide sequence