Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3717 and GFF3718 are separated by 1 nucleotides GFF3718 and GFF3719 are separated by 162 nucleotides GFF3719 and GFF3720 are separated by 265 nucleotides GFF3720 and GFF3721 overlap by 1 nucleotides
GFF3717 - FIG01045846: hypothetical protein, at 22,505 to 22,816
GFF3717
GFF3718 - FIG01045308: hypothetical protein, at 22,818 to 23,039
GFF3718
GFF3719 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 23,202 to 25,109
GFF3719
GFF3720 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 25,375 to 25,926
GFF3720
GFF3721 - Glutathione-regulated potassium-efflux system protein KefB, at 25,926 to 27,731
GFF3721
Position (kb)
23
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1 at 22.293 kb on + strand at 22.293 kb on + strand at 22.293 kb on + strand at 22.294 kb on - strand at 22.294 kb on - strand at 22.294 kb on - strand at 22.296 kb on + strand at 22.296 kb on + strand at 22.321 kb on + strand at 22.328 kb on + strand at 22.416 kb on - strand at 22.464 kb on + strand at 22.465 kb on - strand at 22.481 kb on - strand at 22.481 kb on - strand at 22.522 kb on - strand at 22.667 kb on + strand, within GFF3717 at 22.668 kb on - strand, within GFF3717 at 22.701 kb on - strand, within GFF3717 at 22.756 kb on + strand, within GFF3717 at 22.823 kb on + strand at 22.823 kb on + strand at 22.884 kb on - strand, within GFF3718 at 22.887 kb on + strand, within GFF3718 at 22.887 kb on + strand, within GFF3718 at 22.888 kb on - strand, within GFF3718 at 22.898 kb on + strand, within GFF3718 at 22.990 kb on - strand, within GFF3718 at 23.013 kb on - strand, within GFF3718 at 23.064 kb on - strand at 23.078 kb on - strand at 23.126 kb on + strand at 23.126 kb on + strand at 23.127 kb on - strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.133 kb on - strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.136 kb on - strand at 23.136 kb on - strand at 23.178 kb on - strand at 23.178 kb on - strand at 23.187 kb on + strand at 23.423 kb on + strand, within GFF3719 at 23.448 kb on + strand, within GFF3719 at 23.872 kb on + strand, within GFF3719 at 23.873 kb on - strand, within GFF3719 at 23.919 kb on - strand, within GFF3719 at 23.919 kb on - strand, within GFF3719 at 23.919 kb on - strand, within GFF3719 at 24.254 kb on + strand, within GFF3719 at 24.255 kb on - strand, within GFF3719 at 24.364 kb on + strand, within GFF3719 at 24.364 kb on + strand, within GFF3719 at 24.418 kb on + strand, within GFF3719 at 24.431 kb on - strand, within GFF3719 at 24.600 kb on + strand, within GFF3719 at 24.771 kb on + strand, within GFF3719 at 24.771 kb on + strand, within GFF3719 at 24.771 kb on + strand, within GFF3719 at 24.771 kb on + strand, within GFF3719 at 24.771 kb on + strand, within GFF3719 at 24.772 kb on - strand, within GFF3719 at 24.772 kb on - strand, within GFF3719 at 24.772 kb on - strand, within GFF3719 at 24.772 kb on - strand, within GFF3719 at 24.772 kb on - strand, within GFF3719 at 24.772 kb on - strand, within GFF3719 at 24.783 kb on - strand, within GFF3719 at 24.862 kb on + strand, within GFF3719 at 24.862 kb on + strand, within GFF3719 at 24.863 kb on - strand, within GFF3719 at 24.863 kb on - strand, within GFF3719 at 24.992 kb on + strand at 25.037 kb on + strand at 25.119 kb on + strand at 25.120 kb on - strand at 25.156 kb on + strand at 25.194 kb on - strand at 25.253 kb on + strand at 25.254 kb on - strand at 25.254 kb on - strand at 25.282 kb on + strand at 25.283 kb on - strand at 25.283 kb on - strand at 25.283 kb on - strand at 25.319 kb on + strand at 25.320 kb on - strand at 25.345 kb on - strand at 25.345 kb on - strand at 25.405 kb on + strand at 25.406 kb on - strand at 25.406 kb on - strand at 25.478 kb on + strand, within GFF3720 at 25.548 kb on - strand, within GFF3720 at 25.586 kb on - strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.593 kb on + strand, within GFF3720 at 25.593 kb on + strand, within GFF3720 at 25.594 kb on - strand, within GFF3720 at 25.685 kb on - strand, within GFF3720 at 25.732 kb on + strand, within GFF3720 at 25.824 kb on + strand, within GFF3720 at 25.824 kb on + strand, within GFF3720 at 26.023 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 22,293 + +0.4 22,293 + -0.2 22,293 + -0.3 22,294 - +0.9 22,294 - -0.7 22,294 - +0.0 22,296 + +0.3 22,296 + -0.3 22,321 + +0.7 22,328 + +0.3 22,416 - -0.0 22,464 + -2.6 22,465 - +1.2 22,481 - +0.8 22,481 - +0.3 22,522 - -2.0 22,667 + GFF3717 0.52 -0.5 22,668 - GFF3717 0.52 +0.9 22,701 - GFF3717 0.63 +0.4 22,756 + GFF3717 0.80 +0.1 22,823 + +1.5 22,823 + +0.1 22,884 - GFF3718 0.30 -0.5 22,887 + GFF3718 0.31 -1.4 22,887 + GFF3718 0.31 -0.5 22,888 - GFF3718 0.32 +1.0 22,898 + GFF3718 0.36 -0.4 22,990 - GFF3718 0.77 +0.0 23,013 - GFF3718 0.88 -1.0 23,064 - +0.7 23,078 - +0.2 23,126 + +0.4 23,126 + -0.7 23,127 - +0.2 23,132 + -0.2 23,132 + +0.2 23,132 + +0.4 23,133 - -0.2 23,135 + -0.4 23,135 + +0.1 23,135 + +0.2 23,135 + -0.4 23,135 + -0.1 23,135 + -0.0 23,135 + +0.4 23,135 + -1.7 23,136 - +0.3 23,136 - -0.1 23,178 - -0.9 23,178 - -0.3 23,187 + -0.4 23,423 + GFF3719 0.12 +0.3 23,448 + GFF3719 0.13 +0.4 23,872 + GFF3719 0.35 +1.1 23,873 - GFF3719 0.35 +0.2 23,919 - GFF3719 0.38 +0.5 23,919 - GFF3719 0.38 +0.4 23,919 - GFF3719 0.38 +0.3 24,254 + GFF3719 0.55 +0.2 24,255 - GFF3719 0.55 +0.1 24,364 + GFF3719 0.61 +0.4 24,364 + GFF3719 0.61 +0.0 24,418 + GFF3719 0.64 -0.5 24,431 - GFF3719 0.64 -0.2 24,600 + GFF3719 0.73 +0.1 24,771 + GFF3719 0.82 -0.2 24,771 + GFF3719 0.82 +0.9 24,771 + GFF3719 0.82 +0.2 24,771 + GFF3719 0.82 +1.0 24,771 + GFF3719 0.82 +0.8 24,772 - GFF3719 0.82 +0.2 24,772 - GFF3719 0.82 -0.1 24,772 - GFF3719 0.82 -0.3 24,772 - GFF3719 0.82 -0.6 24,772 - GFF3719 0.82 -0.3 24,772 - GFF3719 0.82 -0.3 24,783 - GFF3719 0.83 +1.3 24,862 + GFF3719 0.87 -0.2 24,862 + GFF3719 0.87 -0.4 24,863 - GFF3719 0.87 +0.0 24,863 - GFF3719 0.87 -0.1 24,992 + -0.1 25,037 + -1.0 25,119 + +0.5 25,120 - -0.1 25,156 + +0.0 25,194 - +0.6 25,253 + -1.3 25,254 - -0.1 25,254 - -0.2 25,282 + -0.0 25,283 - +0.5 25,283 - +0.1 25,283 - -0.6 25,319 + -0.2 25,320 - +0.1 25,345 - +0.3 25,345 - +0.8 25,405 + +0.1 25,406 - +0.3 25,406 - -0.0 25,478 + GFF3720 0.19 +1.1 25,548 - GFF3720 0.31 +0.7 25,586 - GFF3720 0.38 -0.4 25,591 + GFF3720 0.39 -0.1 25,591 + GFF3720 0.39 +0.4 25,591 + GFF3720 0.39 +0.6 25,591 + GFF3720 0.39 -0.1 25,591 + GFF3720 0.39 +0.2 25,591 + GFF3720 0.39 -1.1 25,591 + GFF3720 0.39 +0.3 25,591 + GFF3720 0.39 -0.1 25,592 - GFF3720 0.39 +0.4 25,592 - GFF3720 0.39 -0.5 25,592 - GFF3720 0.39 +0.2 25,592 - GFF3720 0.39 -0.1 25,592 - GFF3720 0.39 -0.0 25,592 - GFF3720 0.39 -0.6 25,592 - GFF3720 0.39 +0.2 25,592 - GFF3720 0.39 +0.5 25,593 + GFF3720 0.39 +0.1 25,593 + GFF3720 0.39 +0.6 25,594 - GFF3720 0.40 -1.0 25,685 - GFF3720 0.56 -0.3 25,732 + GFF3720 0.65 +0.5 25,824 + GFF3720 0.81 +0.3 25,824 + GFF3720 0.81 -0.3 26,023 + +0.0
Or see this region's nucleotide sequence