Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3591 and GFF3592 are separated by 2 nucleotides GFF3592 and GFF3593 are separated by 57 nucleotides GFF3593 and GFF3594 are separated by 15 nucleotides GFF3594 and GFF3595 are separated by 98 nucleotides GFF3595 and GFF3596 are separated by 93 nucleotides GFF3596 and GFF3597 overlap by 23 nucleotides
GFF3591 - Secretion system effector SseD, at 348,604 to 349,107
GFF3591
GFF3592 - Secretion system effector SseE, at 349,110 to 349,526
GFF3592
GFF3593 - Secretion system chaparone SscB, at 349,584 to 350,012
GFF3593
GFF3594 - Type III secretion effector SseF, at 350,028 to 350,810
GFF3594
GFF3595 - Secretion system effector SseG, at 350,909 to 351,496
GFF3595
GFF3596 - Type III secretion protein SsaG, at 351,590 to 351,805
GFF3596
GFF3597 - Type III secretion protein SsaH, at 351,783 to 352,073
GFF3597
Position (kb) 350
351 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 349.168 kb on - strand, within GFF3592 at 349.186 kb on + strand, within GFF3592 at 349.190 kb on - strand, within GFF3592 at 349.230 kb on + strand, within GFF3592 at 349.316 kb on - strand, within GFF3592 at 349.336 kb on + strand, within GFF3592 at 349.578 kb on - strand at 349.578 kb on - strand at 350.107 kb on + strand, within GFF3594 at 350.218 kb on - strand, within GFF3594 at 350.367 kb on + strand, within GFF3594 at 350.603 kb on - strand, within GFF3594 at 350.760 kb on - strand at 350.760 kb on - strand at 350.760 kb on - strand at 350.925 kb on + strand at 351.057 kb on + strand, within GFF3595 at 351.072 kb on + strand, within GFF3595 at 351.160 kb on + strand, within GFF3595 at 351.161 kb on - strand, within GFF3595 at 351.370 kb on + strand, within GFF3595
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 349,168 - GFF3592 0.14 +0.7 349,186 + GFF3592 0.18 +0.4 349,190 - GFF3592 0.19 +0.4 349,230 + GFF3592 0.29 +0.5 349,316 - GFF3592 0.49 +0.1 349,336 + GFF3592 0.54 -0.2 349,578 - +0.4 349,578 - -0.1 350,107 + GFF3594 0.10 +0.9 350,218 - GFF3594 0.24 -2.9 350,367 + GFF3594 0.43 -0.9 350,603 - GFF3594 0.73 -1.3 350,760 - +1.8 350,760 - -2.1 350,760 - +0.4 350,925 + +0.6 351,057 + GFF3595 0.25 +0.0 351,072 + GFF3595 0.28 +0.7 351,160 + GFF3595 0.43 +0.1 351,161 - GFF3595 0.43 -0.8 351,370 + GFF3595 0.78 -1.2
Or see this region's nucleotide sequence