Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3474 and GFF3475 are separated by 391 nucleotides GFF3475 and GFF3476 are separated by 105 nucleotides
GFF3474 - MltA-interacting protein MipA, at 226,242 to 226,988
GFF3474
GFF3475 - Transcriptional regulator, at 227,380 to 228,474
GFF3475
GFF3476 - Putative oxidoreductase YeaE, aldo/keto reductase family, at 228,580 to 229,428
GFF3476
Position (kb) 227
228
229 Strain fitness (log2 ratio) -1
0
1
2 at 226.402 kb on - strand, within GFF3474 at 226.446 kb on + strand, within GFF3474 at 226.447 kb on - strand, within GFF3474 at 226.447 kb on - strand, within GFF3474 at 226.634 kb on + strand, within GFF3474 at 226.655 kb on + strand, within GFF3474 at 226.758 kb on + strand, within GFF3474 at 226.768 kb on + strand, within GFF3474 at 226.850 kb on + strand, within GFF3474 at 226.879 kb on + strand, within GFF3474 at 226.879 kb on + strand, within GFF3474 at 226.880 kb on - strand, within GFF3474 at 226.881 kb on + strand, within GFF3474 at 226.886 kb on + strand, within GFF3474 at 227.075 kb on - strand at 227.108 kb on - strand at 227.285 kb on + strand at 227.288 kb on + strand at 227.625 kb on + strand, within GFF3475 at 227.626 kb on - strand, within GFF3475 at 227.659 kb on - strand, within GFF3475 at 227.827 kb on - strand, within GFF3475 at 227.958 kb on + strand, within GFF3475 at 228.006 kb on - strand, within GFF3475 at 228.029 kb on + strand, within GFF3475 at 228.029 kb on + strand, within GFF3475 at 228.030 kb on - strand, within GFF3475 at 228.261 kb on + strand, within GFF3475 at 228.429 kb on + strand at 228.429 kb on + strand at 228.514 kb on + strand at 228.545 kb on + strand at 228.640 kb on + strand at 228.641 kb on - strand at 228.641 kb on - strand at 228.642 kb on + strand at 228.737 kb on - strand, within GFF3476 at 228.776 kb on + strand, within GFF3476 at 228.901 kb on + strand, within GFF3476 at 228.902 kb on - strand, within GFF3476
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 226,402 - GFF3474 0.21 -0.5 226,446 + GFF3474 0.27 +0.7 226,447 - GFF3474 0.27 +0.1 226,447 - GFF3474 0.27 -0.4 226,634 + GFF3474 0.52 +0.5 226,655 + GFF3474 0.55 +0.4 226,758 + GFF3474 0.69 -0.3 226,768 + GFF3474 0.70 +0.6 226,850 + GFF3474 0.81 +0.7 226,879 + GFF3474 0.85 +0.3 226,879 + GFF3474 0.85 -1.1 226,880 - GFF3474 0.85 -0.7 226,881 + GFF3474 0.86 -0.2 226,886 + GFF3474 0.86 -0.4 227,075 - +0.2 227,108 - -1.6 227,285 + -0.5 227,288 + -0.1 227,625 + GFF3475 0.22 -0.0 227,626 - GFF3475 0.22 +1.2 227,659 - GFF3475 0.25 +1.1 227,827 - GFF3475 0.41 -0.5 227,958 + GFF3475 0.53 -0.6 228,006 - GFF3475 0.57 -1.6 228,029 + GFF3475 0.59 +0.4 228,029 + GFF3475 0.59 +0.1 228,030 - GFF3475 0.59 +2.1 228,261 + GFF3475 0.80 +0.6 228,429 + +0.5 228,429 + +1.1 228,514 + -1.6 228,545 + +0.9 228,640 + -0.1 228,641 - -0.8 228,641 - -0.1 228,642 + -0.0 228,737 - GFF3476 0.18 -0.3 228,776 + GFF3476 0.23 +0.2 228,901 + GFF3476 0.38 +0.6 228,902 - GFF3476 0.38 +1.3
Or see this region's nucleotide sequence