Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3412 and GFF3413 are separated by 39 nucleotides GFF3413 and GFF3414 are separated by 80 nucleotides
GFF3412 - FIG00638667: hypothetical protein, at 176,702 to 178,036
GFF3412
GFF3413 - FIG002776: hypothetical protein, at 178,076 to 179,197
GFF3413
GFF3414 - Sensor protein PhoQ (EC 2.7.13.3), at 179,278 to 180,741
GFF3414
Position (kb)
178
179
180 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 177.076 kb on + strand, within GFF3412 at 177.102 kb on + strand, within GFF3412 at 177.102 kb on + strand, within GFF3412 at 177.108 kb on - strand, within GFF3412 at 177.216 kb on - strand, within GFF3412 at 177.257 kb on + strand, within GFF3412 at 177.295 kb on + strand, within GFF3412 at 177.383 kb on - strand, within GFF3412 at 177.433 kb on + strand, within GFF3412 at 177.434 kb on - strand, within GFF3412 at 177.573 kb on - strand, within GFF3412 at 177.575 kb on - strand, within GFF3412 at 177.611 kb on + strand, within GFF3412 at 177.645 kb on + strand, within GFF3412 at 177.657 kb on + strand, within GFF3412 at 177.728 kb on - strand, within GFF3412 at 177.846 kb on + strand, within GFF3412 at 177.977 kb on + strand at 177.997 kb on + strand at 178.181 kb on - strand at 178.181 kb on - strand at 178.289 kb on + strand, within GFF3413 at 178.441 kb on + strand, within GFF3413 at 178.441 kb on + strand, within GFF3413 at 178.442 kb on - strand, within GFF3413 at 178.454 kb on - strand, within GFF3413 at 178.515 kb on + strand, within GFF3413 at 178.518 kb on + strand, within GFF3413 at 178.519 kb on - strand, within GFF3413 at 178.519 kb on - strand, within GFF3413 at 178.523 kb on - strand, within GFF3413 at 178.523 kb on - strand, within GFF3413 at 178.629 kb on + strand, within GFF3413 at 178.799 kb on - strand, within GFF3413 at 178.802 kb on - strand, within GFF3413 at 178.877 kb on - strand, within GFF3413 at 179.126 kb on + strand at 179.184 kb on - strand at 179.191 kb on - strand at 179.218 kb on + strand at 179.218 kb on + strand at 179.373 kb on - strand at 179.425 kb on + strand, within GFF3414 at 179.582 kb on - strand, within GFF3414 at 179.675 kb on + strand, within GFF3414 at 179.741 kb on - strand, within GFF3414 at 179.877 kb on + strand, within GFF3414 at 180.128 kb on - strand, within GFF3414 at 180.153 kb on - strand, within GFF3414 at 180.153 kb on - strand, within GFF3414 at 180.174 kb on + strand, within GFF3414 at 180.177 kb on - strand, within GFF3414
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 177,076 + GFF3412 0.28 +2.4 177,102 + GFF3412 0.30 -0.5 177,102 + GFF3412 0.30 +0.1 177,108 - GFF3412 0.30 -0.9 177,216 - GFF3412 0.39 -0.7 177,257 + GFF3412 0.42 -0.9 177,295 + GFF3412 0.44 -0.4 177,383 - GFF3412 0.51 -0.7 177,433 + GFF3412 0.55 -1.4 177,434 - GFF3412 0.55 -1.0 177,573 - GFF3412 0.65 -0.3 177,575 - GFF3412 0.65 -0.9 177,611 + GFF3412 0.68 +0.3 177,645 + GFF3412 0.71 +0.2 177,657 + GFF3412 0.72 +0.0 177,728 - GFF3412 0.77 -0.0 177,846 + GFF3412 0.86 -0.5 177,977 + -0.6 177,997 + +1.3 178,181 - +0.8 178,181 - -1.4 178,289 + GFF3413 0.19 -0.8 178,441 + GFF3413 0.33 +0.4 178,441 + GFF3413 0.33 -3.1 178,442 - GFF3413 0.33 -0.2 178,454 - GFF3413 0.34 +0.3 178,515 + GFF3413 0.39 -0.9 178,518 + GFF3413 0.39 +1.4 178,519 - GFF3413 0.39 -0.1 178,519 - GFF3413 0.39 -0.4 178,523 - GFF3413 0.40 -1.8 178,523 - GFF3413 0.40 -0.6 178,629 + GFF3413 0.49 -0.2 178,799 - GFF3413 0.64 +0.2 178,802 - GFF3413 0.65 -0.3 178,877 - GFF3413 0.71 -0.3 179,126 + +0.1 179,184 - -1.1 179,191 - -0.1 179,218 + +0.1 179,218 + -0.5 179,373 - +0.8 179,425 + GFF3414 0.10 +1.5 179,582 - GFF3414 0.21 +1.4 179,675 + GFF3414 0.27 -0.6 179,741 - GFF3414 0.32 +0.5 179,877 + GFF3414 0.41 +0.2 180,128 - GFF3414 0.58 +0.1 180,153 - GFF3414 0.60 +0.6 180,153 - GFF3414 0.60 +0.7 180,174 + GFF3414 0.61 +0.5 180,177 - GFF3414 0.61 +0.6
Or see this region's nucleotide sequence