Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3279 and GFF3280 are separated by 1 nucleotides GFF3280 and GFF3281 are separated by 9 nucleotides GFF3281 and GFF3282 are separated by 142 nucleotides
GFF3279 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 51,373 to 52,839
GFF3279
GFF3280 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 52,841 to 53,692
GFF3280
GFF3281 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 53,702 to 54,082
GFF3281
GFF3282 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 54,225 to 55,028
GFF3282
Position (kb) 52
53
54 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.066 kb on - strand, within GFF3279 at 52.244 kb on - strand, within GFF3279 at 52.368 kb on + strand, within GFF3279 at 52.369 kb on - strand, within GFF3279 at 52.611 kb on + strand, within GFF3279 at 52.759 kb on + strand at 52.762 kb on - strand at 53.038 kb on + strand, within GFF3280 at 53.039 kb on - strand, within GFF3280 at 53.069 kb on - strand, within GFF3280 at 53.108 kb on + strand, within GFF3280 at 53.115 kb on - strand, within GFF3280 at 53.238 kb on - strand, within GFF3280 at 53.351 kb on + strand, within GFF3280 at 53.352 kb on - strand, within GFF3280 at 53.607 kb on - strand at 53.713 kb on + strand at 54.048 kb on - strand at 54.072 kb on + strand at 54.212 kb on - strand at 54.224 kb on + strand at 54.267 kb on - strand at 54.329 kb on + strand, within GFF3282 at 54.342 kb on + strand, within GFF3282 at 54.343 kb on - strand, within GFF3282 at 54.462 kb on + strand, within GFF3282 at 54.463 kb on - strand, within GFF3282 at 54.622 kb on - strand, within GFF3282
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 52,066 - GFF3279 0.47 +0.6 52,244 - GFF3279 0.59 +1.4 52,368 + GFF3279 0.68 +0.8 52,369 - GFF3279 0.68 -0.1 52,611 + GFF3279 0.84 +1.7 52,759 + +0.7 52,762 - +0.1 53,038 + GFF3280 0.23 -2.0 53,039 - GFF3280 0.23 +0.3 53,069 - GFF3280 0.27 -0.6 53,108 + GFF3280 0.31 +0.4 53,115 - GFF3280 0.32 -1.0 53,238 - GFF3280 0.47 +1.3 53,351 + GFF3280 0.60 -1.2 53,352 - GFF3280 0.60 -0.4 53,607 - +2.3 53,713 + -0.1 54,048 - +0.3 54,072 + +1.0 54,212 - -0.2 54,224 + +0.6 54,267 - +0.4 54,329 + GFF3282 0.13 +0.4 54,342 + GFF3282 0.15 -0.5 54,343 - GFF3282 0.15 +2.3 54,462 + GFF3282 0.29 -0.4 54,463 - GFF3282 0.30 +0.7 54,622 - GFF3282 0.49 +0.6
Or see this region's nucleotide sequence