Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3186 and GFF3187 are separated by 85 nucleotides GFF3187 and GFF3188 are separated by 211 nucleotides GFF3188 and GFF3189 are separated by 88 nucleotides
GFF3186 - 23S rRNA (guanine-N-2-) -methyltransferase rlmG (EC 2.1.1.-), at 193,489 to 194,625
GFF3186
GFF3187 - COG1451: Predicted metal-dependent hydrolase, at 194,711 to 195,208
GFF3187
GFF3188 - SanA protein, at 195,420 to 196,052
GFF3188
GFF3189 - Possible oxidoreductase, at 196,141 to 197,139
GFF3189
Position (kb) 194
195
196 Strain fitness (log2 ratio) -1
0
1
2 at 193.858 kb on + strand, within GFF3186 at 193.859 kb on - strand, within GFF3186 at 193.859 kb on - strand, within GFF3186 at 194.037 kb on + strand, within GFF3186 at 194.097 kb on + strand, within GFF3186 at 194.188 kb on - strand, within GFF3186 at 194.214 kb on + strand, within GFF3186 at 194.368 kb on - strand, within GFF3186 at 194.615 kb on - strand at 194.680 kb on - strand at 194.722 kb on - strand at 194.722 kb on - strand at 194.851 kb on - strand, within GFF3187 at 194.851 kb on - strand, within GFF3187 at 194.852 kb on + strand, within GFF3187 at 194.852 kb on + strand, within GFF3187 at 194.860 kb on + strand, within GFF3187 at 194.908 kb on + strand, within GFF3187 at 194.908 kb on + strand, within GFF3187 at 194.908 kb on + strand, within GFF3187 at 194.919 kb on - strand, within GFF3187 at 194.919 kb on - strand, within GFF3187 at 195.146 kb on + strand, within GFF3187 at 195.255 kb on + strand at 195.255 kb on + strand at 195.315 kb on + strand at 195.416 kb on - strand at 195.551 kb on - strand, within GFF3188 at 195.591 kb on - strand, within GFF3188 at 195.804 kb on - strand, within GFF3188 at 195.915 kb on + strand, within GFF3188 at 195.916 kb on - strand, within GFF3188 at 195.990 kb on + strand at 196.010 kb on + strand at 196.101 kb on - strand at 196.141 kb on - strand at 196.157 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 193,858 + GFF3186 0.32 +0.6 193,859 - GFF3186 0.33 +0.1 193,859 - GFF3186 0.33 +0.4 194,037 + GFF3186 0.48 +1.0 194,097 + GFF3186 0.53 -0.5 194,188 - GFF3186 0.61 -0.3 194,214 + GFF3186 0.64 +0.2 194,368 - GFF3186 0.77 -0.2 194,615 - +1.4 194,680 - -0.1 194,722 - +0.3 194,722 - +0.0 194,851 - GFF3187 0.28 -0.0 194,851 - GFF3187 0.28 +0.8 194,852 + GFF3187 0.28 -0.7 194,852 + GFF3187 0.28 +0.5 194,860 + GFF3187 0.30 -0.0 194,908 + GFF3187 0.40 +1.0 194,908 + GFF3187 0.40 -0.8 194,908 + GFF3187 0.40 +1.0 194,919 - GFF3187 0.42 +0.6 194,919 - GFF3187 0.42 -0.1 195,146 + GFF3187 0.87 +0.1 195,255 + -0.3 195,255 + +0.3 195,315 + +0.1 195,416 - +0.4 195,551 - GFF3188 0.21 +0.3 195,591 - GFF3188 0.27 +0.3 195,804 - GFF3188 0.61 +1.7 195,915 + GFF3188 0.78 -0.6 195,916 - GFF3188 0.78 +0.6 195,990 + +0.4 196,010 + +2.3 196,101 - +0.2 196,141 - -0.5 196,157 + +1.5
Or see this region's nucleotide sequence