Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2746 and GFF2747 are separated by 358 nucleotides GFF2747 and GFF2748 are separated by 265 nucleotides GFF2748 and GFF2749 are separated by 476 nucleotides
GFF2746 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 265,226 to 266,857
GFF2746
GFF2747 - hypothetical protein, at 267,216 to 267,356
GFF2747
GFF2748 - Putative membrane protein, at 267,622 to 268,269
GFF2748
GFF2749 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 268,746 to 271,424
GFF2749
Position (kb)
267
268
269 Strain fitness (log2 ratio)
-2
-1
0
1 at 266.636 kb on + strand, within GFF2746 at 266.728 kb on + strand at 266.729 kb on - strand at 266.729 kb on - strand at 266.851 kb on + strand at 266.881 kb on - strand at 266.881 kb on - strand at 266.907 kb on - strand at 266.943 kb on - strand at 266.970 kb on - strand at 267.026 kb on + strand at 267.071 kb on - strand at 267.415 kb on + strand at 267.551 kb on + strand at 267.561 kb on + strand at 267.589 kb on + strand at 267.624 kb on - strand at 268.050 kb on + strand, within GFF2748 at 268.352 kb on - strand at 268.363 kb on + strand at 268.364 kb on - strand at 268.368 kb on - strand at 268.401 kb on - strand at 268.406 kb on + strand at 268.463 kb on - strand at 268.514 kb on + strand at 268.583 kb on - strand at 268.970 kb on + strand at 268.974 kb on + strand at 268.985 kb on + strand at 268.985 kb on + strand at 269.269 kb on + strand, within GFF2749
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 266,636 + GFF2746 0.86 -0.5 266,728 + -0.3 266,729 - -2.3 266,729 - -0.5 266,851 + +0.1 266,881 - -0.3 266,881 - +0.2 266,907 - -0.0 266,943 - -1.5 266,970 - -0.8 267,026 + +0.4 267,071 - -0.3 267,415 + +1.2 267,551 + +0.2 267,561 + +0.9 267,589 + -0.9 267,624 - +1.3 268,050 + GFF2748 0.66 -0.8 268,352 - +0.1 268,363 + -0.2 268,364 - -2.1 268,368 - +0.2 268,401 - +0.1 268,406 + -0.7 268,463 - +0.1 268,514 + +1.1 268,583 - -0.8 268,970 + -0.4 268,974 + +0.1 268,985 + +0.5 268,985 + +0.2 269,269 + GFF2749 0.20 +1.2
Or see this region's nucleotide sequence