Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2659 and GFF2660 are separated by 85 nucleotides GFF2660 and GFF2661 are separated by 43 nucleotides GFF2661 and GFF2662 are separated by 141 nucleotides
GFF2659 - Mechanosensitive ion channel, at 182,390 to 183,421
GFF2659
GFF2660 - Putative transcriptional regulator, at 183,507 to 184,109
GFF2660
GFF2661 - FIG01045442: hypothetical protein, at 184,153 to 184,839
GFF2661
GFF2662 - FIG01045240: hypothetical protein, at 184,981 to 185,205
GFF2662
Position (kb) 183
184
185 Strain fitness (log2 ratio) -2
-1
0
1 at 182.767 kb on + strand, within GFF2659 at 182.902 kb on + strand, within GFF2659 at 182.930 kb on - strand, within GFF2659 at 183.032 kb on + strand, within GFF2659 at 183.254 kb on - strand, within GFF2659 at 183.261 kb on - strand, within GFF2659 at 183.383 kb on + strand at 183.568 kb on + strand, within GFF2660 at 183.569 kb on - strand, within GFF2660 at 183.643 kb on - strand, within GFF2660 at 183.643 kb on - strand, within GFF2660 at 183.908 kb on + strand, within GFF2660 at 183.909 kb on - strand, within GFF2660 at 183.913 kb on - strand, within GFF2660 at 183.913 kb on - strand, within GFF2660 at 184.161 kb on + strand at 184.218 kb on - strand at 184.218 kb on - strand at 184.300 kb on - strand, within GFF2661 at 184.331 kb on + strand, within GFF2661 at 184.332 kb on - strand, within GFF2661 at 184.463 kb on - strand, within GFF2661 at 184.463 kb on - strand, within GFF2661 at 184.465 kb on - strand, within GFF2661 at 184.483 kb on + strand, within GFF2661 at 184.519 kb on + strand, within GFF2661 at 184.570 kb on - strand, within GFF2661 at 184.620 kb on + strand, within GFF2661 at 184.677 kb on - strand, within GFF2661 at 184.702 kb on - strand, within GFF2661 at 184.702 kb on - strand, within GFF2661 at 184.756 kb on + strand, within GFF2661
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 182,767 + GFF2659 0.37 +0.0 182,902 + GFF2659 0.50 +0.2 182,930 - GFF2659 0.52 -0.3 183,032 + GFF2659 0.62 -0.3 183,254 - GFF2659 0.84 -0.2 183,261 - GFF2659 0.84 +0.3 183,383 + +0.2 183,568 + GFF2660 0.10 -0.8 183,569 - GFF2660 0.10 -1.9 183,643 - GFF2660 0.23 -0.3 183,643 - GFF2660 0.23 -0.8 183,908 + GFF2660 0.67 -0.9 183,909 - GFF2660 0.67 +0.7 183,913 - GFF2660 0.67 -0.9 183,913 - GFF2660 0.67 -1.3 184,161 + +0.1 184,218 - +0.9 184,218 - -0.1 184,300 - GFF2661 0.21 -0.0 184,331 + GFF2661 0.26 -0.0 184,332 - GFF2661 0.26 +0.7 184,463 - GFF2661 0.45 -0.9 184,463 - GFF2661 0.45 +0.1 184,465 - GFF2661 0.45 +0.9 184,483 + GFF2661 0.48 +0.7 184,519 + GFF2661 0.53 +0.4 184,570 - GFF2661 0.61 -0.4 184,620 + GFF2661 0.68 -0.0 184,677 - GFF2661 0.76 -0.1 184,702 - GFF2661 0.80 -0.9 184,702 - GFF2661 0.80 +0.1 184,756 + GFF2661 0.88 -0.6
Or see this region's nucleotide sequence