Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2514

Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2512 and GFF2513 overlap by 1 nucleotidesGFF2513 and GFF2514 are separated by 223 nucleotidesGFF2514 and GFF2515 are separated by 55 nucleotides GFF2512 - Glutaminase (EC 3.5.1.2), at 28,261 to 29,187 GFF2512 GFF2513 - FIG00637950: hypothetical protein, at 29,187 to 29,546 GFF2513 GFF2514 - FIG00638206: hypothetical protein, at 29,770 to 30,717 GFF2514 GFF2515 - putative outer membrane protein, at 30,773 to 31,300 GFF2515 Position (kb) 29 30 31Strain fitness (log2 ratio) -2 -1 0 1at 28.835 kb on - strand, within GFF2512at 28.843 kb on + strand, within GFF2512at 28.844 kb on - strand, within GFF2512at 29.392 kb on + strand, within GFF2513at 29.494 kb on - strand, within GFF2513at 29.495 kb on + strand, within GFF2513at 29.527 kb on + strandat 29.579 kb on - strandat 29.601 kb on - strandat 29.621 kb on + strandat 29.622 kb on - strandat 29.622 kb on - strandat 29.907 kb on + strand, within GFF2514at 29.919 kb on + strand, within GFF2514at 29.919 kb on + strand, within GFF2514at 29.974 kb on - strand, within GFF2514at 29.974 kb on - strand, within GFF2514at 30.276 kb on + strand, within GFF2514at 30.277 kb on - strand, within GFF2514at 30.508 kb on - strand, within GFF2514at 30.563 kb on + strand, within GFF2514at 30.756 kb on - strandat 30.775 kb on - strandat 30.848 kb on + strand, within GFF2515at 31.175 kb on - strand, within GFF2515at 31.303 kb on + strandat 31.304 kb on - strandat 31.350 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI
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28,835 - GFF2512 0.62 -0.8
28,843 + GFF2512 0.63 -0.2
28,844 - GFF2512 0.63 -1.0
29,392 + GFF2513 0.57 -1.1
29,494 - GFF2513 0.85 -2.3
29,495 + GFF2513 0.86 +1.5
29,527 + +0.4
29,579 - +0.4
29,601 - -0.9
29,621 + +0.7
29,622 - -0.6
29,622 - -1.2
29,907 + GFF2514 0.14 -1.4
29,919 + GFF2514 0.16 +1.2
29,919 + GFF2514 0.16 +0.1
29,974 - GFF2514 0.22 -1.1
29,974 - GFF2514 0.22 -1.0
30,276 + GFF2514 0.53 -2.4
30,277 - GFF2514 0.53 +0.5
30,508 - GFF2514 0.78 -0.8
30,563 + GFF2514 0.84 -1.0
30,756 - +0.4
30,775 - +0.1
30,848 + GFF2515 0.14 -0.3
31,175 - GFF2515 0.76 -0.1
31,303 + +0.7
31,304 - +0.4
31,350 + -0.1

Or see this region's nucleotide sequence