Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2415 and GFF2416 are separated by 120 nucleotides GFF2416 and GFF2417 overlap by 1 nucleotides GFF2417 and GFF2418 overlap by 8 nucleotides GFF2418 and GFF2419 overlap by 4 nucleotides
GFF2415 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 28,554 to 28,985
GFF2415
GFF2416 - Putative polyferredoxin, at 29,106 to 29,969
GFF2416
GFF2417 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 29,969 to 30,778
GFF2417
GFF2418 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 30,771 to 31,400
GFF2418
GFF2419 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 31,397 to 33,775
GFF2419
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-2
-1
0
1 at 28.990 kb on - strand at 29.031 kb on + strand at 29.094 kb on + strand at 29.094 kb on + strand at 29.207 kb on + strand, within GFF2416 at 29.208 kb on - strand, within GFF2416 at 29.217 kb on - strand, within GFF2416 at 29.220 kb on - strand, within GFF2416 at 29.349 kb on - strand, within GFF2416 at 29.649 kb on - strand, within GFF2416 at 29.811 kb on + strand, within GFF2416 at 29.812 kb on - strand, within GFF2416 at 29.825 kb on + strand, within GFF2416 at 29.977 kb on + strand at 29.980 kb on + strand at 29.981 kb on - strand at 29.981 kb on - strand at 29.991 kb on + strand at 30.001 kb on + strand at 30.117 kb on + strand, within GFF2417 at 30.190 kb on + strand, within GFF2417 at 30.358 kb on + strand, within GFF2417 at 30.388 kb on + strand, within GFF2417 at 30.389 kb on - strand, within GFF2417 at 30.390 kb on + strand, within GFF2417 at 30.390 kb on + strand, within GFF2417 at 30.410 kb on + strand, within GFF2417 at 30.510 kb on + strand, within GFF2417 at 30.563 kb on + strand, within GFF2417 at 30.563 kb on + strand, within GFF2417 at 30.563 kb on + strand, within GFF2417 at 30.693 kb on + strand, within GFF2417 at 30.768 kb on - strand at 30.992 kb on + strand, within GFF2418 at 31.055 kb on - strand, within GFF2418 at 31.405 kb on + strand at 31.406 kb on - strand at 31.406 kb on - strand at 31.607 kb on - strand at 31.633 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 28,990 - -0.1 29,031 + +0.1 29,094 + +0.2 29,094 + +0.2 29,207 + GFF2416 0.12 +0.0 29,208 - GFF2416 0.12 -0.8 29,217 - GFF2416 0.13 -0.7 29,220 - GFF2416 0.13 -0.2 29,349 - GFF2416 0.28 +0.8 29,649 - GFF2416 0.63 -2.6 29,811 + GFF2416 0.82 -0.3 29,812 - GFF2416 0.82 +0.5 29,825 + GFF2416 0.83 +0.1 29,977 + -0.4 29,980 + +0.6 29,981 - -0.1 29,981 - -0.0 29,991 + -0.7 30,001 + -0.8 30,117 + GFF2417 0.18 -0.4 30,190 + GFF2417 0.27 -0.1 30,358 + GFF2417 0.48 -0.4 30,388 + GFF2417 0.52 +0.1 30,389 - GFF2417 0.52 +1.2 30,390 + GFF2417 0.52 +0.4 30,390 + GFF2417 0.52 +0.3 30,410 + GFF2417 0.54 +0.1 30,510 + GFF2417 0.67 +0.8 30,563 + GFF2417 0.73 +0.6 30,563 + GFF2417 0.73 -1.6 30,563 + GFF2417 0.73 +0.1 30,693 + GFF2417 0.89 +0.2 30,768 - +0.4 30,992 + GFF2418 0.35 -0.9 31,055 - GFF2418 0.45 +0.1 31,405 + -0.1 31,406 - +1.1 31,406 - -0.6 31,607 - -0.4 31,633 + +0.6
Or see this region's nucleotide sequence