Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2412 and GFF2413 are separated by 26 nucleotides GFF2413 and GFF2414 are separated by 291 nucleotides
GFF2412 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1), at 24,740 to 25,858
GFF2412
GFF2413 - FIG021952: putative membrane protein, at 25,885 to 26,889
GFF2413
GFF2414 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 27,181 to 28,347
GFF2414
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 25.912 kb on - strand at 26.017 kb on - strand, within GFF2413 at 26.042 kb on - strand, within GFF2413 at 26.907 kb on - strand at 26.977 kb on - strand at 26.995 kb on + strand at 26.995 kb on + strand at 26.996 kb on - strand at 26.996 kb on - strand at 26.996 kb on - strand at 26.996 kb on - strand at 27.057 kb on + strand at 27.060 kb on - strand at 27.060 kb on - strand at 27.064 kb on - strand at 27.128 kb on - strand at 27.128 kb on - strand at 27.152 kb on - strand at 27.180 kb on - strand at 27.187 kb on - strand at 27.216 kb on + strand at 27.241 kb on - strand at 27.308 kb on - strand, within GFF2414 at 27.418 kb on - strand, within GFF2414 at 27.423 kb on + strand, within GFF2414 at 27.423 kb on + strand, within GFF2414 at 27.424 kb on - strand, within GFF2414 at 27.501 kb on - strand, within GFF2414 at 27.501 kb on - strand, within GFF2414 at 27.501 kb on - strand, within GFF2414 at 27.582 kb on - strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.635 kb on - strand, within GFF2414 at 27.635 kb on - strand, within GFF2414 at 27.658 kb on + strand, within GFF2414 at 27.658 kb on + strand, within GFF2414 at 27.788 kb on - strand, within GFF2414
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 25,912 - -0.3 26,017 - GFF2413 0.13 -0.8 26,042 - GFF2413 0.16 -0.7 26,907 - +0.5 26,977 - +0.5 26,995 + +0.2 26,995 + +0.4 26,996 - +0.4 26,996 - +0.0 26,996 - +0.0 26,996 - +0.4 27,057 + +0.8 27,060 - +0.3 27,060 - +0.7 27,064 - +0.6 27,128 - -0.0 27,128 - +0.4 27,152 - +2.1 27,180 - +0.3 27,187 - +0.1 27,216 + +0.5 27,241 - +0.2 27,308 - GFF2414 0.11 +0.6 27,418 - GFF2414 0.20 -0.1 27,423 + GFF2414 0.21 -0.1 27,423 + GFF2414 0.21 -0.5 27,424 - GFF2414 0.21 +0.1 27,501 - GFF2414 0.27 -0.2 27,501 - GFF2414 0.27 -1.4 27,501 - GFF2414 0.27 +0.1 27,582 - GFF2414 0.34 +0.2 27,634 + GFF2414 0.39 +0.2 27,634 + GFF2414 0.39 -3.1 27,634 + GFF2414 0.39 +0.2 27,634 + GFF2414 0.39 +0.2 27,635 - GFF2414 0.39 -1.2 27,635 - GFF2414 0.39 -0.0 27,658 + GFF2414 0.41 +0.7 27,658 + GFF2414 0.41 -0.2 27,788 - GFF2414 0.52 +1.0
Or see this region's nucleotide sequence