Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2296 and GFF2297 overlap by 1 nucleotides GFF2297 and GFF2298 are separated by 7 nucleotides
GFF2296 - Autoinducer 2 (AI-2) ABC transport system, membrane channel protein LsrC, at 83,954 to 84,997
GFF2296
GFF2297 - Autoinducer 2 (AI-2) ABC transport system, membrane channel protein LsrD, at 84,997 to 85,995
GFF2297
GFF2298 - Autoinducer 2 (AI-2) ABC transport system, periplasmic AI-2 binding protein LsrB, at 86,003 to 87,046
GFF2298
Position (kb) 84
85
86 Strain fitness (log2 ratio) -2
-1
0
1 at 84.013 kb on - strand at 84.044 kb on + strand at 84.147 kb on + strand, within GFF2296 at 84.209 kb on + strand, within GFF2296 at 84.241 kb on - strand, within GFF2296 at 84.243 kb on + strand, within GFF2296 at 84.244 kb on - strand, within GFF2296 at 84.355 kb on - strand, within GFF2296 at 84.469 kb on + strand, within GFF2296 at 84.841 kb on - strand, within GFF2296 at 84.922 kb on + strand at 85.043 kb on + strand at 85.298 kb on + strand, within GFF2297 at 85.323 kb on + strand, within GFF2297 at 85.407 kb on + strand, within GFF2297 at 85.557 kb on + strand, within GFF2297 at 85.636 kb on + strand, within GFF2297 at 85.636 kb on + strand, within GFF2297 at 85.780 kb on + strand, within GFF2297 at 86.378 kb on - strand, within GFF2298 at 86.402 kb on + strand, within GFF2298 at 86.402 kb on + strand, within GFF2298 at 86.403 kb on - strand, within GFF2298 at 86.403 kb on - strand, within GFF2298 at 86.583 kb on + strand, within GFF2298 at 86.583 kb on + strand, within GFF2298 at 86.603 kb on + strand, within GFF2298 at 86.729 kb on - strand, within GFF2298 at 86.772 kb on + strand, within GFF2298 at 86.773 kb on - strand, within GFF2298 at 86.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 84,013 - +0.1 84,044 + -0.1 84,147 + GFF2296 0.18 -0.1 84,209 + GFF2296 0.24 +0.5 84,241 - GFF2296 0.27 +0.4 84,243 + GFF2296 0.28 +1.0 84,244 - GFF2296 0.28 +0.8 84,355 - GFF2296 0.38 -0.1 84,469 + GFF2296 0.49 -0.4 84,841 - GFF2296 0.85 +0.2 84,922 + -1.8 85,043 + -1.1 85,298 + GFF2297 0.30 +0.1 85,323 + GFF2297 0.33 +0.1 85,407 + GFF2297 0.41 -0.6 85,557 + GFF2297 0.56 +0.7 85,636 + GFF2297 0.64 -1.4 85,636 + GFF2297 0.64 -0.7 85,780 + GFF2297 0.78 +1.0 86,378 - GFF2298 0.36 -0.2 86,402 + GFF2298 0.38 -0.5 86,402 + GFF2298 0.38 +1.3 86,403 - GFF2298 0.38 -1.2 86,403 - GFF2298 0.38 +0.6 86,583 + GFF2298 0.56 -0.2 86,583 + GFF2298 0.56 -1.0 86,603 + GFF2298 0.57 -0.3 86,729 - GFF2298 0.70 -0.0 86,772 + GFF2298 0.74 -0.2 86,773 - GFF2298 0.74 -0.5 86,945 - -0.3
Or see this region's nucleotide sequence