Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2276 and GFF2277 are separated by 69 nucleotides GFF2277 and GFF2278 are separated by 11 nucleotides GFF2278 and GFF2279 are separated by 50 nucleotides
GFF2276 - Manganese superoxide dismutase (EC 1.15.1.1), at 65,463 to 66,083
GFF2276
GFF2277 - Uncharacterized protein conserved in bacteria, at 66,153 to 66,842
GFF2277
GFF2278 - Putative inner membrane protein, at 66,854 to 67,249
GFF2278
GFF2279 - Copper sensory histidine kinase CpxA, at 67,300 to 68,673
GFF2279
Position (kb) 66
67
68 Strain fitness (log2 ratio) -1
0
1 at 65.985 kb on + strand, within GFF2276 at 65.986 kb on - strand, within GFF2276 at 66.176 kb on + strand at 66.177 kb on - strand at 66.322 kb on + strand, within GFF2277 at 66.323 kb on - strand, within GFF2277 at 66.449 kb on + strand, within GFF2277 at 66.462 kb on + strand, within GFF2277 at 66.522 kb on - strand, within GFF2277 at 66.692 kb on + strand, within GFF2277 at 66.692 kb on + strand, within GFF2277 at 66.693 kb on - strand, within GFF2277 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.938 kb on + strand, within GFF2278 at 66.939 kb on - strand, within GFF2278 at 66.939 kb on - strand, within GFF2278 at 66.940 kb on + strand, within GFF2278 at 67.001 kb on - strand, within GFF2278 at 67.080 kb on - strand, within GFF2278 at 67.534 kb on - strand, within GFF2279 at 67.536 kb on + strand, within GFF2279 at 67.576 kb on - strand, within GFF2279 at 67.605 kb on + strand, within GFF2279 at 67.644 kb on - strand, within GFF2279 at 67.736 kb on - strand, within GFF2279 at 67.924 kb on + strand, within GFF2279 at 68.134 kb on + strand, within GFF2279 at 68.135 kb on - strand, within GFF2279 at 68.243 kb on + strand, within GFF2279
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 65,985 + GFF2276 0.84 +1.6 65,986 - GFF2276 0.84 -0.4 66,176 + -0.7 66,177 - -0.3 66,322 + GFF2277 0.24 -0.6 66,323 - GFF2277 0.25 +0.4 66,449 + GFF2277 0.43 -0.1 66,462 + GFF2277 0.45 +0.4 66,522 - GFF2277 0.53 -0.3 66,692 + GFF2277 0.78 +0.1 66,692 + GFF2277 0.78 +0.1 66,693 - GFF2277 0.78 +0.7 66,938 + GFF2278 0.21 -0.1 66,938 + GFF2278 0.21 +0.1 66,938 + GFF2278 0.21 +0.9 66,938 + GFF2278 0.21 -1.3 66,939 - GFF2278 0.21 +0.5 66,939 - GFF2278 0.21 +0.2 66,940 + GFF2278 0.22 -0.2 67,001 - GFF2278 0.37 +0.4 67,080 - GFF2278 0.57 +1.2 67,534 - GFF2279 0.17 +1.6 67,536 + GFF2279 0.17 +1.7 67,576 - GFF2279 0.20 -0.2 67,605 + GFF2279 0.22 -1.1 67,644 - GFF2279 0.25 +0.6 67,736 - GFF2279 0.32 -0.1 67,924 + GFF2279 0.45 -0.4 68,134 + GFF2279 0.61 +0.4 68,135 - GFF2279 0.61 +1.1 68,243 + GFF2279 0.69 +0.0
Or see this region's nucleotide sequence