Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2142 and GFF2143 are separated by 7 nucleotides GFF2143 and GFF2144 are separated by 5 nucleotides GFF2144 and GFF2145 are separated by 171 nucleotides GFF2145 and GFF2146 are separated by 59 nucleotides GFF2146 and GFF2147 are separated by 103 nucleotides
GFF2142 - probable membrane protein YPO3684, at 33,803 to 34,006
GFF2142
GFF2143 - Membrane fusion component of tripartite multidrug resistance system, at 34,014 to 34,946
GFF2143
GFF2144 - FUSARIC ACID RESISTANCE PROTEIN FUSB / FUSARIC ACID RESISTANCE PROTEIN FUSC, at 34,952 to 36,919
GFF2144
GFF2145 - probable ribonuclease inhibitor YPO3690, at 37,091 to 37,363
GFF2145
GFF2146 - FIG01045534: hypothetical protein, at 37,423 to 37,689
GFF2146
GFF2147 - probable exported protein YPO3518, at 37,793 to 38,056
GFF2147
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 34.053 kb on - strand at 34.107 kb on + strand at 34.107 kb on + strand at 34.161 kb on - strand, within GFF2143 at 34.195 kb on + strand, within GFF2143 at 34.195 kb on + strand, within GFF2143 at 34.196 kb on - strand, within GFF2143 at 34.294 kb on + strand, within GFF2143 at 34.295 kb on - strand, within GFF2143 at 34.303 kb on - strand, within GFF2143 at 34.310 kb on - strand, within GFF2143 at 34.481 kb on + strand, within GFF2143 at 34.599 kb on + strand, within GFF2143 at 34.599 kb on + strand, within GFF2143 at 34.694 kb on + strand, within GFF2143 at 34.728 kb on - strand, within GFF2143 at 34.757 kb on - strand, within GFF2143 at 35.026 kb on - strand at 35.162 kb on - strand, within GFF2144 at 35.270 kb on - strand, within GFF2144 at 35.286 kb on + strand, within GFF2144 at 35.286 kb on + strand, within GFF2144 at 35.307 kb on - strand, within GFF2144 at 35.329 kb on + strand, within GFF2144 at 35.329 kb on + strand, within GFF2144 at 35.330 kb on - strand, within GFF2144 at 35.330 kb on - strand, within GFF2144 at 35.500 kb on - strand, within GFF2144 at 35.521 kb on - strand, within GFF2144 at 35.722 kb on + strand, within GFF2144 at 35.756 kb on - strand, within GFF2144 at 35.901 kb on - strand, within GFF2144 at 35.904 kb on + strand, within GFF2144 at 35.905 kb on - strand, within GFF2144 at 35.905 kb on - strand, within GFF2144 at 35.984 kb on - strand, within GFF2144 at 36.060 kb on + strand, within GFF2144 at 36.356 kb on - strand, within GFF2144 at 36.431 kb on - strand, within GFF2144 at 36.467 kb on - strand, within GFF2144 at 36.499 kb on + strand, within GFF2144 at 36.500 kb on - strand, within GFF2144 at 36.500 kb on - strand, within GFF2144 at 36.547 kb on - strand, within GFF2144 at 36.547 kb on - strand, within GFF2144 at 36.578 kb on + strand, within GFF2144 at 36.745 kb on + strand at 36.766 kb on + strand at 36.780 kb on - strand at 36.812 kb on + strand at 36.813 kb on - strand at 36.826 kb on - strand at 36.829 kb on + strand at 36.885 kb on + strand at 36.886 kb on - strand at 36.889 kb on + strand at 36.889 kb on + strand at 36.889 kb on + strand at 36.890 kb on - strand at 36.890 kb on - strand at 36.890 kb on - strand at 36.890 kb on - strand at 36.890 kb on - strand at 36.893 kb on + strand at 36.961 kb on + strand at 36.963 kb on + strand at 36.964 kb on - strand at 36.964 kb on - strand at 36.964 kb on - strand at 37.038 kb on - strand at 37.038 kb on - strand at 37.047 kb on + strand at 37.047 kb on + strand at 37.047 kb on + strand at 37.047 kb on + strand at 37.048 kb on - strand at 37.048 kb on - strand at 37.048 kb on - strand at 37.048 kb on - strand at 37.230 kb on + strand, within GFF2145 at 37.341 kb on + strand at 37.428 kb on + strand at 37.429 kb on - strand at 37.431 kb on - strand at 37.455 kb on + strand, within GFF2146 at 37.455 kb on + strand, within GFF2146 at 37.456 kb on - strand, within GFF2146 at 37.488 kb on + strand, within GFF2146 at 37.488 kb on + strand, within GFF2146 at 37.489 kb on - strand, within GFF2146 at 37.489 kb on - strand, within GFF2146 at 37.489 kb on - strand, within GFF2146 at 37.545 kb on + strand, within GFF2146 at 37.706 kb on - strand at 37.708 kb on - strand at 37.726 kb on + strand at 37.772 kb on - strand at 37.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 34,053 - -1.3 34,107 + +0.2 34,107 + -0.4 34,161 - GFF2143 0.16 +0.2 34,195 + GFF2143 0.19 -0.1 34,195 + GFF2143 0.19 +0.5 34,196 - GFF2143 0.20 +0.2 34,294 + GFF2143 0.30 +0.1 34,295 - GFF2143 0.30 +0.5 34,303 - GFF2143 0.31 +0.3 34,310 - GFF2143 0.32 +0.5 34,481 + GFF2143 0.50 -0.2 34,599 + GFF2143 0.63 +0.3 34,599 + GFF2143 0.63 -0.3 34,694 + GFF2143 0.73 -0.5 34,728 - GFF2143 0.77 -0.1 34,757 - GFF2143 0.80 -1.1 35,026 - -0.1 35,162 - GFF2144 0.11 +0.2 35,270 - GFF2144 0.16 +0.1 35,286 + GFF2144 0.17 -2.1 35,286 + GFF2144 0.17 +0.5 35,307 - GFF2144 0.18 +0.4 35,329 + GFF2144 0.19 +1.3 35,329 + GFF2144 0.19 +0.4 35,330 - GFF2144 0.19 -0.0 35,330 - GFF2144 0.19 -0.0 35,500 - GFF2144 0.28 +0.6 35,521 - GFF2144 0.29 +0.8 35,722 + GFF2144 0.39 +0.4 35,756 - GFF2144 0.41 -0.6 35,901 - GFF2144 0.48 +0.2 35,904 + GFF2144 0.48 -0.7 35,905 - GFF2144 0.48 -0.3 35,905 - GFF2144 0.48 -0.8 35,984 - GFF2144 0.52 -0.4 36,060 + GFF2144 0.56 +0.2 36,356 - GFF2144 0.71 -1.5 36,431 - GFF2144 0.75 +0.3 36,467 - GFF2144 0.77 -0.2 36,499 + GFF2144 0.79 -0.4 36,500 - GFF2144 0.79 -0.0 36,500 - GFF2144 0.79 +0.4 36,547 - GFF2144 0.81 +4.0 36,547 - GFF2144 0.81 -0.2 36,578 + GFF2144 0.83 +0.2 36,745 + -1.1 36,766 + -0.2 36,780 - -0.5 36,812 + -2.0 36,813 - -1.6 36,826 - +0.7 36,829 + +0.7 36,885 + +0.4 36,886 - +3.4 36,889 + +1.5 36,889 + -0.7 36,889 + -0.8 36,890 - +0.8 36,890 - +0.5 36,890 - +0.1 36,890 - -0.9 36,890 - +0.7 36,893 + -0.1 36,961 + +0.1 36,963 + +0.4 36,964 - -0.4 36,964 - -0.7 36,964 - -0.1 37,038 - -0.4 37,038 - -0.2 37,047 + +0.3 37,047 + +1.4 37,047 + +0.2 37,047 + -0.3 37,048 - -0.3 37,048 - -2.1 37,048 - -1.2 37,048 - -1.2 37,230 + GFF2145 0.51 -0.6 37,341 + -0.2 37,428 + +0.0 37,429 - -0.1 37,431 - +0.3 37,455 + GFF2146 0.12 +0.1 37,455 + GFF2146 0.12 +0.7 37,456 - GFF2146 0.12 +0.2 37,488 + GFF2146 0.24 -0.8 37,488 + GFF2146 0.24 +0.1 37,489 - GFF2146 0.25 +0.4 37,489 - GFF2146 0.25 +0.7 37,489 - GFF2146 0.25 -1.9 37,545 + GFF2146 0.46 -1.1 37,706 - -0.6 37,708 - -1.4 37,726 + -0.5 37,772 - +0.5 37,774 - +0.3
Or see this region's nucleotide sequence