Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2095 and GFF2096 are separated by 114 nucleotides GFF2096 and GFF2097 are separated by 53 nucleotides GFF2097 and GFF2098 are separated by 72 nucleotides
GFF2095 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 2,674 to 4,029
GFF2095
GFF2096 - Protein acetyltransferase, at 4,144 to 6,804
GFF2096
GFF2097 - hypothetical protein, at 6,858 to 7,538
GFF2097
GFF2098 - Thioredoxin 2 (EC 1.8.1.8), at 7,611 to 8,030
GFF2098
Position (kb) 4
5
6
7 Strain fitness (log2 ratio) -1
0
1
2 at 4.137 kb on - strand at 4.387 kb on + strand at 4.387 kb on + strand at 4.388 kb on - strand at 4.388 kb on - strand at 4.388 kb on - strand at 4.388 kb on - strand at 4.551 kb on - strand, within GFF2096 at 5.128 kb on + strand, within GFF2096 at 5.257 kb on + strand, within GFF2096 at 5.258 kb on - strand, within GFF2096 at 5.272 kb on - strand, within GFF2096 at 5.490 kb on + strand, within GFF2096 at 5.491 kb on - strand, within GFF2096 at 5.496 kb on + strand, within GFF2096 at 5.497 kb on - strand, within GFF2096 at 5.528 kb on + strand, within GFF2096 at 5.634 kb on + strand, within GFF2096 at 5.635 kb on - strand, within GFF2096 at 5.799 kb on + strand, within GFF2096 at 5.800 kb on - strand, within GFF2096 at 5.803 kb on + strand, within GFF2096 at 5.804 kb on - strand, within GFF2096 at 5.804 kb on - strand, within GFF2096 at 5.958 kb on + strand, within GFF2096 at 6.172 kb on + strand, within GFF2096 at 6.173 kb on - strand, within GFF2096 at 6.185 kb on + strand, within GFF2096 at 6.453 kb on + strand, within GFF2096 at 6.454 kb on - strand, within GFF2096 at 6.454 kb on - strand, within GFF2096 at 6.764 kb on + strand at 6.979 kb on - strand, within GFF2097 at 7.094 kb on + strand, within GFF2097 at 7.106 kb on + strand, within GFF2097 at 7.106 kb on + strand, within GFF2097 at 7.228 kb on + strand, within GFF2097 at 7.229 kb on - strand, within GFF2097 at 7.406 kb on + strand, within GFF2097 at 7.530 kb on - strand at 7.670 kb on + strand, within GFF2098
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 4,137 - -0.2 4,387 + -0.3 4,387 + -0.6 4,388 - +0.5 4,388 - -0.4 4,388 - +1.1 4,388 - +0.1 4,551 - GFF2096 0.15 +0.4 5,128 + GFF2096 0.37 -0.0 5,257 + GFF2096 0.42 -1.7 5,258 - GFF2096 0.42 +0.2 5,272 - GFF2096 0.42 +0.4 5,490 + GFF2096 0.51 +0.4 5,491 - GFF2096 0.51 -0.1 5,496 + GFF2096 0.51 -1.3 5,497 - GFF2096 0.51 -0.1 5,528 + GFF2096 0.52 -0.9 5,634 + GFF2096 0.56 -0.1 5,635 - GFF2096 0.56 +0.3 5,799 + GFF2096 0.62 +0.8 5,800 - GFF2096 0.62 +0.8 5,803 + GFF2096 0.62 +1.0 5,804 - GFF2096 0.62 -1.6 5,804 - GFF2096 0.62 -1.2 5,958 + GFF2096 0.68 -1.3 6,172 + GFF2096 0.76 +0.4 6,173 - GFF2096 0.76 -0.0 6,185 + GFF2096 0.77 +0.5 6,453 + GFF2096 0.87 -1.0 6,454 - GFF2096 0.87 +2.1 6,454 - GFF2096 0.87 +0.5 6,764 + -0.4 6,979 - GFF2097 0.18 +1.0 7,094 + GFF2097 0.35 -0.2 7,106 + GFF2097 0.36 +0.4 7,106 + GFF2097 0.36 -0.0 7,228 + GFF2097 0.54 -0.4 7,229 - GFF2097 0.54 -1.2 7,406 + GFF2097 0.80 -0.2 7,530 - +0.1 7,670 + GFF2098 0.14 -0.9
Or see this region's nucleotide sequence