Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1945 and GFF1946 are separated by 51 nucleotides GFF1946 and GFF1947 are separated by 32 nucleotides GFF1947 and GFF1948 overlap by 1 nucleotides GFF1948 and GFF1949 overlap by 1 nucleotides
GFF1945 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 136,386 to 138,386
GFF1945
GFF1946 - FIG00639460: hypothetical protein, at 138,438 to 138,713
GFF1946
GFF1947 - Putative transporting ATPase, at 138,746 to 139,294
GFF1947
GFF1948 - Putative membrane protein YfcA, at 139,294 to 140,103
GFF1948
GFF1949 - Murein endopeptidase, at 140,103 to 140,927
GFF1949
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 137.840 kb on + strand, within GFF1945 at 137.881 kb on + strand, within GFF1945 at 137.896 kb on - strand, within GFF1945 at 137.918 kb on + strand, within GFF1945 at 137.919 kb on - strand, within GFF1945 at 137.934 kb on - strand, within GFF1945 at 138.133 kb on - strand, within GFF1945 at 138.174 kb on - strand, within GFF1945 at 138.202 kb on - strand at 138.216 kb on - strand at 138.291 kb on - strand at 138.362 kb on - strand at 138.553 kb on + strand, within GFF1946 at 138.554 kb on - strand, within GFF1946 at 138.729 kb on + strand at 138.730 kb on - strand at 138.804 kb on + strand, within GFF1947 at 138.869 kb on + strand, within GFF1947 at 139.228 kb on + strand, within GFF1947 at 139.340 kb on + strand at 139.340 kb on + strand at 139.341 kb on - strand at 139.441 kb on + strand, within GFF1948 at 139.572 kb on + strand, within GFF1948 at 139.573 kb on - strand, within GFF1948 at 139.583 kb on - strand, within GFF1948 at 139.641 kb on - strand, within GFF1948 at 139.871 kb on - strand, within GFF1948 at 139.878 kb on + strand, within GFF1948 at 139.879 kb on - strand, within GFF1948 at 140.014 kb on - strand, within GFF1948 at 140.080 kb on - strand at 140.080 kb on - strand at 140.110 kb on - strand at 140.244 kb on + strand, within GFF1949
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 137,840 + GFF1945 0.73 -0.0 137,881 + GFF1945 0.75 -0.2 137,896 - GFF1945 0.75 +0.0 137,918 + GFF1945 0.77 -0.9 137,919 - GFF1945 0.77 -2.0 137,934 - GFF1945 0.77 +1.6 138,133 - GFF1945 0.87 +1.1 138,174 - GFF1945 0.89 -0.3 138,202 - -0.1 138,216 - -0.3 138,291 - -0.1 138,362 - -1.8 138,553 + GFF1946 0.42 -2.8 138,554 - GFF1946 0.42 -0.5 138,729 + +1.2 138,730 - -1.1 138,804 + GFF1947 0.11 +0.4 138,869 + GFF1947 0.22 -1.7 139,228 + GFF1947 0.88 -0.7 139,340 + -0.8 139,340 + -0.2 139,341 - -0.1 139,441 + GFF1948 0.18 -1.0 139,572 + GFF1948 0.34 -3.0 139,573 - GFF1948 0.34 -0.9 139,583 - GFF1948 0.36 +0.0 139,641 - GFF1948 0.43 -0.7 139,871 - GFF1948 0.71 +0.1 139,878 + GFF1948 0.72 +2.1 139,879 - GFF1948 0.72 -0.0 140,014 - GFF1948 0.89 -0.2 140,080 - +0.3 140,080 - +0.4 140,110 - -0.0 140,244 + GFF1949 0.17 -1.4
Or see this region's nucleotide sequence