Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1657 and GFF1658 are separated by 53 nucleotides GFF1658 and GFF1659 are separated by 236 nucleotides GFF1659 and GFF1660 overlap by 1 nucleotides
GFF1657 - Penicillin-binding protein 2 (PBP-2), at 40,940 to 42,811
GFF1657
GFF1658 - Rhamnogalacturonides degradation protein RhiN, at 42,865 to 44,004
GFF1658
GFF1659 - Flagellar protein FlhE, at 44,241 to 44,633
GFF1659
GFF1660 - Flagellar biosynthesis protein FlhA, at 44,633 to 46,711
GFF1660
Position (kb) 42
43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 41.930 kb on + strand, within GFF1657 at 41.930 kb on + strand, within GFF1657 at 41.931 kb on - strand, within GFF1657 at 41.931 kb on - strand, within GFF1657 at 41.931 kb on - strand, within GFF1657 at 41.963 kb on - strand, within GFF1657 at 42.117 kb on - strand, within GFF1657 at 42.117 kb on - strand, within GFF1657 at 42.118 kb on + strand, within GFF1657 at 42.118 kb on + strand, within GFF1657 at 42.118 kb on + strand, within GFF1657 at 42.119 kb on - strand, within GFF1657 at 42.200 kb on + strand, within GFF1657 at 42.201 kb on - strand, within GFF1657 at 42.524 kb on - strand, within GFF1657 at 42.580 kb on - strand, within GFF1657 at 42.606 kb on - strand, within GFF1657 at 42.606 kb on - strand, within GFF1657 at 42.606 kb on - strand, within GFF1657 at 42.884 kb on + strand at 42.928 kb on - strand at 42.928 kb on - strand at 43.017 kb on + strand, within GFF1658 at 43.140 kb on - strand, within GFF1658 at 43.140 kb on - strand, within GFF1658 at 43.342 kb on - strand, within GFF1658 at 43.425 kb on - strand, within GFF1658 at 43.425 kb on - strand, within GFF1658 at 43.465 kb on - strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.782 kb on - strand, within GFF1658 at 44.129 kb on + strand at 44.129 kb on + strand at 44.292 kb on - strand, within GFF1659 at 44.542 kb on - strand, within GFF1659 at 44.547 kb on + strand, within GFF1659 at 44.547 kb on + strand, within GFF1659 at 44.548 kb on - strand, within GFF1659 at 44.742 kb on - strand at 44.967 kb on - strand, within GFF1660 at 44.978 kb on + strand, within GFF1660
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 41,930 + GFF1657 0.53 +0.3 41,930 + GFF1657 0.53 +0.0 41,931 - GFF1657 0.53 -0.0 41,931 - GFF1657 0.53 -0.6 41,931 - GFF1657 0.53 +0.9 41,963 - GFF1657 0.55 +0.8 42,117 - GFF1657 0.63 +0.3 42,117 - GFF1657 0.63 +0.3 42,118 + GFF1657 0.63 -1.6 42,118 + GFF1657 0.63 +0.5 42,118 + GFF1657 0.63 -0.6 42,119 - GFF1657 0.63 +1.3 42,200 + GFF1657 0.67 -1.4 42,201 - GFF1657 0.67 +0.4 42,524 - GFF1657 0.85 -3.1 42,580 - GFF1657 0.88 -0.2 42,606 - GFF1657 0.89 -0.2 42,606 - GFF1657 0.89 -0.1 42,606 - GFF1657 0.89 -0.4 42,884 + +1.7 42,928 - +0.2 42,928 - -2.1 43,017 + GFF1658 0.13 +1.1 43,140 - GFF1658 0.24 -0.0 43,140 - GFF1658 0.24 -1.6 43,342 - GFF1658 0.42 -2.3 43,425 - GFF1658 0.49 +0.7 43,425 - GFF1658 0.49 -0.1 43,465 - GFF1658 0.53 -1.6 43,781 + GFF1658 0.80 +0.0 43,781 + GFF1658 0.80 +1.7 43,781 + GFF1658 0.80 +0.4 43,782 - GFF1658 0.80 +0.4 44,129 + +0.7 44,129 + +0.3 44,292 - GFF1659 0.13 +0.1 44,542 - GFF1659 0.77 -0.1 44,547 + GFF1659 0.78 -0.1 44,547 + GFF1659 0.78 +0.6 44,548 - GFF1659 0.78 +1.1 44,742 - +0.3 44,967 - GFF1660 0.16 -1.1 44,978 + GFF1660 0.17 -0.1
Or see this region's nucleotide sequence