Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1392 and GFF1393 are separated by 2 nucleotides GFF1393 and GFF1394 are separated by 575 nucleotides GFF1394 and GFF1395 are separated by 132 nucleotides
GFF1392 - Phage lysin, at 36,647 to 37,252
GFF1392
GFF1393 - Putative inner membrane protein, at 37,255 to 37,542
GFF1393
GFF1394 - putative cytoplasmic protein, at 38,118 to 38,465
GFF1394
GFF1395 - Aspartokinase (EC 2.7.2.4), at 38,598 to 39,947
GFF1395
Position (kb) 38
39 Strain fitness (log2 ratio) -2
-1
0
1 at 37.235 kb on + strand at 37.236 kb on - strand at 37.236 kb on - strand at 37.236 kb on - strand at 37.239 kb on + strand at 37.240 kb on - strand at 37.384 kb on - strand, within GFF1393 at 37.436 kb on + strand, within GFF1393 at 37.489 kb on + strand, within GFF1393 at 37.489 kb on + strand, within GFF1393 at 37.611 kb on - strand at 38.113 kb on - strand at 38.211 kb on - strand, within GFF1394 at 38.304 kb on - strand, within GFF1394 at 38.485 kb on - strand at 38.559 kb on - strand at 38.569 kb on - strand at 38.607 kb on + strand at 38.739 kb on - strand, within GFF1395 at 38.854 kb on - strand, within GFF1395 at 38.922 kb on - strand, within GFF1395 at 38.932 kb on - strand, within GFF1395 at 38.939 kb on + strand, within GFF1395 at 38.942 kb on + strand, within GFF1395 at 39.075 kb on - strand, within GFF1395 at 39.156 kb on + strand, within GFF1395 at 39.175 kb on - strand, within GFF1395 at 39.250 kb on - strand, within GFF1395 at 39.269 kb on - strand, within GFF1395 at 39.339 kb on - strand, within GFF1395 at 39.339 kb on - strand, within GFF1395 at 39.339 kb on - strand, within GFF1395 at 39.408 kb on - strand, within GFF1395
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 37,235 + +0.1 37,236 - +0.1 37,236 - +0.7 37,236 - +0.0 37,239 + -0.4 37,240 - -0.1 37,384 - GFF1393 0.45 -0.2 37,436 + GFF1393 0.63 +0.4 37,489 + GFF1393 0.81 -0.4 37,489 + GFF1393 0.81 -1.5 37,611 - -1.4 38,113 - -1.4 38,211 - GFF1394 0.27 -0.5 38,304 - GFF1394 0.53 -0.9 38,485 - -0.1 38,559 - -0.7 38,569 - -0.0 38,607 + -0.2 38,739 - GFF1395 0.10 -0.9 38,854 - GFF1395 0.19 +0.3 38,922 - GFF1395 0.24 -2.4 38,932 - GFF1395 0.25 +1.2 38,939 + GFF1395 0.25 +0.3 38,942 + GFF1395 0.25 +0.9 39,075 - GFF1395 0.35 -0.1 39,156 + GFF1395 0.41 -0.1 39,175 - GFF1395 0.43 -0.5 39,250 - GFF1395 0.48 -0.2 39,269 - GFF1395 0.50 -0.5 39,339 - GFF1395 0.55 +0.8 39,339 - GFF1395 0.55 -0.4 39,339 - GFF1395 0.55 +0.1 39,408 - GFF1395 0.60 -0.1
Or see this region's nucleotide sequence