Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1351 and GFF1352 are separated by 31 nucleotides GFF1352 and GFF1353 are separated by 3 nucleotides GFF1353 and GFF1354 overlap by 25 nucleotides
GFF1351 - Malate synthase (EC 2.3.3.9), at 2,164 to 3,768
GFF1351
GFF1352 - Isocitrate lyase (EC 4.1.3.1), at 3,800 to 5,104
GFF1352
GFF1353 - hypothetical protein, at 5,108 to 5,230
GFF1353
GFF1354 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 5,206 to 6,957
GFF1354
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -1
0
1
2 at 2.800 kb on + strand, within GFF1351 at 3.633 kb on - strand at 3.767 kb on - strand at 3.778 kb on + strand at 3.832 kb on - strand at 4.064 kb on + strand, within GFF1352 at 4.065 kb on - strand, within GFF1352 at 4.116 kb on - strand, within GFF1352 at 4.247 kb on + strand, within GFF1352 at 4.555 kb on + strand, within GFF1352 at 4.586 kb on + strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.747 kb on + strand, within GFF1352 at 4.839 kb on - strand, within GFF1352 at 4.846 kb on + strand, within GFF1352 at 4.904 kb on + strand, within GFF1352 at 4.932 kb on - strand, within GFF1352 at 5.019 kb on - strand at 5.019 kb on - strand at 5.148 kb on + strand, within GFF1353 at 5.406 kb on + strand, within GFF1354 at 5.407 kb on - strand, within GFF1354 at 5.407 kb on - strand, within GFF1354 at 5.710 kb on - strand, within GFF1354 at 5.730 kb on + strand, within GFF1354 at 5.731 kb on - strand, within GFF1354 at 5.809 kb on + strand, within GFF1354 at 5.844 kb on - strand, within GFF1354 at 5.917 kb on - strand, within GFF1354 at 5.977 kb on - strand, within GFF1354 at 5.977 kb on - strand, within GFF1354 at 5.984 kb on - strand, within GFF1354 at 6.056 kb on - strand, within GFF1354 at 6.058 kb on - strand, within GFF1354
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 2,800 + GFF1351 0.40 -0.7 3,633 - +0.0 3,767 - +0.2 3,778 + -0.2 3,832 - -0.1 4,064 + GFF1352 0.20 +1.0 4,065 - GFF1352 0.20 +0.4 4,116 - GFF1352 0.24 -0.2 4,247 + GFF1352 0.34 +0.5 4,555 + GFF1352 0.58 -0.1 4,586 + GFF1352 0.60 -0.5 4,673 - GFF1352 0.67 -0.5 4,673 - GFF1352 0.67 +1.8 4,673 - GFF1352 0.67 +0.6 4,747 + GFF1352 0.73 +0.4 4,839 - GFF1352 0.80 +0.3 4,846 + GFF1352 0.80 +0.6 4,904 + GFF1352 0.85 -0.3 4,932 - GFF1352 0.87 +0.6 5,019 - -0.8 5,019 - +0.3 5,148 + GFF1353 0.33 -0.3 5,406 + GFF1354 0.11 +1.4 5,407 - GFF1354 0.11 +0.6 5,407 - GFF1354 0.11 -0.2 5,710 - GFF1354 0.29 +2.6 5,730 + GFF1354 0.30 -0.6 5,731 - GFF1354 0.30 +0.6 5,809 + GFF1354 0.34 -0.2 5,844 - GFF1354 0.36 +1.0 5,917 - GFF1354 0.41 +0.5 5,977 - GFF1354 0.44 -0.2 5,977 - GFF1354 0.44 -1.0 5,984 - GFF1354 0.44 -0.8 6,056 - GFF1354 0.49 +1.7 6,058 - GFF1354 0.49 +0.1
Or see this region's nucleotide sequence