Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu6124 and Atu6125 overlap by 14 nucleotides Atu6125 and Atu6126 overlap by 26 nucleotides Atu6126 and Atu6127 are separated by 258 nucleotides Atu6127 and Atu6128 overlap by 4 nucleotides Atu6128 and Atu6129 are separated by 88 nucleotides
Atu6124: Atu6124 - conjugative transfer protein, Coupling protein, at 141,249 to 143,225
Atu6124
Atu6125: Atu6125 - conjugative transfer protein, Dtr system, at 143,212 to 143,457
Atu6125
Atu6126: Atu6126 - conjugal transfer protein, Dtr system, at 143,432 to 143,728
Atu6126
Atu6127: Atu6127 - OriT nicking enzyme, Dtr system, at 143,987 to 147,289
Atu6127
Atu6128: Atu6128 - Conjugative pilin processing protease, at 147,286 to 147,816
Atu6128
Atu6129: Atu6129 - conjugal transfer protein, at 147,905 to 149,002
Atu6129
Position (kb)
143
144
145
146
147
148 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 143.018 kb on - strand, within Atu6124 at 143.068 kb on + strand at 143.068 kb on + strand at 143.068 kb on + strand at 143.068 kb on + strand at 143.068 kb on + strand at 143.076 kb on - strand at 143.076 kb on - strand at 143.083 kb on + strand at 143.099 kb on + strand at 143.114 kb on + strand at 143.243 kb on - strand, within Atu6125 at 143.259 kb on - strand, within Atu6125 at 143.338 kb on + strand, within Atu6125 at 143.338 kb on + strand, within Atu6125 at 143.343 kb on + strand, within Atu6125 at 143.343 kb on + strand, within Atu6125 at 143.346 kb on - strand, within Atu6125 at 143.403 kb on + strand, within Atu6125 at 143.430 kb on + strand, within Atu6125 at 143.430 kb on + strand, within Atu6125 at 143.440 kb on + strand at 143.450 kb on + strand at 143.458 kb on - strand at 143.485 kb on + strand, within Atu6126 at 143.485 kb on + strand, within Atu6126 at 143.493 kb on - strand, within Atu6126 at 143.493 kb on - strand, within Atu6126 at 143.493 kb on - strand, within Atu6126 at 143.493 kb on - strand, within Atu6126 at 143.534 kb on + strand, within Atu6126 at 143.567 kb on + strand, within Atu6126 at 143.584 kb on + strand, within Atu6126 at 143.585 kb on - strand, within Atu6126 at 143.585 kb on - strand, within Atu6126 at 143.598 kb on + strand, within Atu6126 at 143.621 kb on - strand, within Atu6126 at 143.621 kb on - strand, within Atu6126 at 143.658 kb on + strand, within Atu6126 at 143.670 kb on + strand, within Atu6126 at 143.718 kb on + strand at 143.732 kb on + strand at 143.805 kb on + strand at 143.805 kb on + strand at 143.813 kb on - strand at 143.835 kb on + strand at 143.891 kb on - strand at 143.916 kb on + strand at 143.938 kb on + strand at 144.001 kb on + strand at 144.059 kb on + strand at 144.059 kb on + strand at 144.162 kb on + strand at 144.185 kb on + strand at 144.195 kb on + strand at 144.218 kb on + strand at 144.240 kb on - strand at 144.301 kb on + strand at 144.301 kb on + strand at 144.306 kb on + strand at 144.309 kb on - strand at 144.309 kb on - strand at 144.311 kb on + strand at 144.318 kb on + strand, within Atu6127 at 144.356 kb on - strand, within Atu6127 at 144.356 kb on - strand, within Atu6127 at 144.358 kb on + strand, within Atu6127 at 144.372 kb on - strand, within Atu6127 at 144.395 kb on + strand, within Atu6127 at 144.484 kb on - strand, within Atu6127 at 144.564 kb on - strand, within Atu6127 at 144.597 kb on - strand, within Atu6127 at 144.650 kb on - strand, within Atu6127 at 144.671 kb on - strand, within Atu6127 at 144.880 kb on + strand, within Atu6127 at 144.951 kb on - strand, within Atu6127 at 144.953 kb on - strand, within Atu6127 at 144.992 kb on - strand, within Atu6127 at 145.013 kb on - strand, within Atu6127 at 145.094 kb on + strand, within Atu6127 at 145.190 kb on + strand, within Atu6127 at 145.216 kb on + strand, within Atu6127 at 145.233 kb on - strand, within Atu6127 at 145.245 kb on + strand, within Atu6127 at 145.253 kb on - strand, within Atu6127 at 145.342 kb on + strand, within Atu6127 at 145.344 kb on + strand, within Atu6127 at 145.347 kb on + strand, within Atu6127 at 145.347 kb on + strand, within Atu6127 at 145.358 kb on + strand, within Atu6127 at 145.379 kb on - strand, within Atu6127 at 145.428 kb on + strand, within Atu6127 at 145.462 kb on + strand, within Atu6127 at 145.507 kb on + strand, within Atu6127 at 145.555 kb on + strand, within Atu6127 at 145.623 kb on + strand, within Atu6127 at 145.640 kb on + strand, within Atu6127 at 145.668 kb on - strand, within Atu6127 at 145.793 kb on + strand, within Atu6127 at 145.798 kb on + strand, within Atu6127 at 145.858 kb on + strand, within Atu6127 at 145.918 kb on + strand, within Atu6127 at 145.929 kb on + strand, within Atu6127 at 145.961 kb on + strand, within Atu6127 at 145.963 kb on + strand, within Atu6127 at 146.072 kb on - strand, within Atu6127 at 146.095 kb on - strand, within Atu6127 at 146.099 kb on + strand, within Atu6127 at 146.099 kb on + strand, within Atu6127 at 146.109 kb on + strand, within Atu6127 at 146.110 kb on + strand, within Atu6127 at 146.110 kb on + strand, within Atu6127 at 146.117 kb on - strand, within Atu6127 at 146.117 kb on - strand, within Atu6127 at 146.118 kb on - strand, within Atu6127 at 146.118 kb on - strand, within Atu6127 at 146.154 kb on + strand, within Atu6127 at 146.180 kb on + strand, within Atu6127 at 146.180 kb on + strand, within Atu6127 at 146.199 kb on - strand, within Atu6127 at 146.230 kb on + strand, within Atu6127 at 146.376 kb on + strand, within Atu6127 at 146.382 kb on - strand, within Atu6127 at 146.421 kb on + strand, within Atu6127 at 146.429 kb on - strand, within Atu6127 at 146.456 kb on - strand, within Atu6127 at 146.531 kb on - strand, within Atu6127 at 146.677 kb on - strand, within Atu6127 at 146.677 kb on - strand, within Atu6127 at 146.716 kb on + strand, within Atu6127 at 146.755 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.794 kb on + strand, within Atu6127 at 146.941 kb on + strand, within Atu6127 at 146.941 kb on + strand, within Atu6127 at 146.947 kb on - strand, within Atu6127 at 147.050 kb on - strand at 147.077 kb on + strand at 147.077 kb on + strand at 147.082 kb on + strand at 147.141 kb on + strand at 147.164 kb on + strand at 147.212 kb on - strand at 147.236 kb on - strand at 147.288 kb on + strand at 147.301 kb on + strand at 147.301 kb on + strand at 147.331 kb on + strand at 147.339 kb on - strand, within Atu6128 at 147.339 kb on - strand, within Atu6128 at 147.469 kb on + strand, within Atu6128 at 147.515 kb on + strand, within Atu6128 at 147.525 kb on + strand, within Atu6128 at 147.535 kb on - strand, within Atu6128 at 147.535 kb on - strand, within Atu6128 at 147.536 kb on + strand, within Atu6128 at 147.627 kb on + strand, within Atu6128 at 147.651 kb on + strand, within Atu6128 at 147.673 kb on + strand, within Atu6128 at 147.749 kb on + strand, within Atu6128 at 147.757 kb on - strand, within Atu6128 at 147.768 kb on - strand at 147.768 kb on - strand at 147.783 kb on - strand at 147.796 kb on + strand at 147.803 kb on + strand at 147.804 kb on - strand at 147.806 kb on - strand at 147.806 kb on - strand at 147.835 kb on + strand at 147.902 kb on + strand at 148.005 kb on - strand at 148.023 kb on - strand, within Atu6129 at 148.049 kb on - strand, within Atu6129 at 148.101 kb on - strand, within Atu6129 at 148.101 kb on - strand, within Atu6129 at 148.141 kb on + strand, within Atu6129 at 148.141 kb on + strand, within Atu6129 at 148.149 kb on - strand, within Atu6129 at 148.198 kb on - strand, within Atu6129 at 148.223 kb on + strand, within Atu6129 at 148.274 kb on + strand, within Atu6129
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 143,018 - Atu6124 0.89 +0.0 143,068 + +1.6 143,068 + +2.6 143,068 + +2.1 143,068 + +0.2 143,068 + +1.0 143,076 - -0.9 143,076 - +0.8 143,083 + +1.7 143,099 + +0.5 143,114 + -0.6 143,243 - Atu6125 0.13 -0.9 143,259 - Atu6125 0.19 -0.9 143,338 + Atu6125 0.51 +0.4 143,338 + Atu6125 0.51 +0.6 143,343 + Atu6125 0.53 +0.1 143,343 + Atu6125 0.53 +1.5 143,346 - Atu6125 0.54 +0.7 143,403 + Atu6125 0.78 +1.0 143,430 + Atu6125 0.89 -0.0 143,430 + Atu6125 0.89 -1.0 143,440 + +0.3 143,450 + +0.4 143,458 - +0.0 143,485 + Atu6126 0.18 +1.7 143,485 + Atu6126 0.18 +0.2 143,493 - Atu6126 0.21 -0.3 143,493 - Atu6126 0.21 +0.9 143,493 - Atu6126 0.21 +1.5 143,493 - Atu6126 0.21 +1.4 143,534 + Atu6126 0.34 +1.1 143,567 + Atu6126 0.45 +1.0 143,584 + Atu6126 0.51 +2.3 143,585 - Atu6126 0.52 +0.9 143,585 - Atu6126 0.52 +0.3 143,598 + Atu6126 0.56 +1.3 143,621 - Atu6126 0.64 -0.7 143,621 - Atu6126 0.64 -0.9 143,658 + Atu6126 0.76 +2.9 143,670 + Atu6126 0.80 -0.5 143,718 + +2.6 143,732 + +0.2 143,805 + -0.8 143,805 + -0.8 143,813 - +0.2 143,835 + +0.3 143,891 - -0.1 143,916 + +1.1 143,938 + +1.1 144,001 + -0.5 144,059 + +1.9 144,059 + -0.6 144,162 + -0.9 144,185 + +1.5 144,195 + +2.3 144,218 + -0.2 144,240 - +3.0 144,301 + +1.1 144,301 + +0.4 144,306 + -0.7 144,309 - +0.8 144,309 - +0.1 144,311 + +3.3 144,318 + Atu6127 0.10 -0.1 144,356 - Atu6127 0.11 -0.8 144,356 - Atu6127 0.11 +1.7 144,358 + Atu6127 0.11 +0.0 144,372 - Atu6127 0.12 +0.6 144,395 + Atu6127 0.12 -0.2 144,484 - Atu6127 0.15 -1.2 144,564 - Atu6127 0.17 +1.4 144,597 - Atu6127 0.18 -1.6 144,650 - Atu6127 0.20 +0.1 144,671 - Atu6127 0.21 +1.7 144,880 + Atu6127 0.27 -0.4 144,951 - Atu6127 0.29 -0.6 144,953 - Atu6127 0.29 -0.7 144,992 - Atu6127 0.30 -0.8 145,013 - Atu6127 0.31 -1.4 145,094 + Atu6127 0.34 -1.1 145,190 + Atu6127 0.36 +0.3 145,216 + Atu6127 0.37 +1.8 145,233 - Atu6127 0.38 +2.1 145,245 + Atu6127 0.38 +0.8 145,253 - Atu6127 0.38 +1.5 145,342 + Atu6127 0.41 +1.8 145,344 + Atu6127 0.41 -0.6 145,347 + Atu6127 0.41 +0.1 145,347 + Atu6127 0.41 +0.1 145,358 + Atu6127 0.42 +0.1 145,379 - Atu6127 0.42 +0.4 145,428 + Atu6127 0.44 +1.2 145,462 + Atu6127 0.45 +1.3 145,507 + Atu6127 0.46 +2.7 145,555 + Atu6127 0.47 +0.9 145,623 + Atu6127 0.50 +0.5 145,640 + Atu6127 0.50 +0.3 145,668 - Atu6127 0.51 +2.1 145,793 + Atu6127 0.55 +1.2 145,798 + Atu6127 0.55 +1.4 145,858 + Atu6127 0.57 +2.5 145,918 + Atu6127 0.58 +0.2 145,929 + Atu6127 0.59 +0.1 145,961 + Atu6127 0.60 +0.2 145,963 + Atu6127 0.60 +0.8 146,072 - Atu6127 0.63 +0.3 146,095 - Atu6127 0.64 +0.8 146,099 + Atu6127 0.64 +1.1 146,099 + Atu6127 0.64 -0.4 146,109 + Atu6127 0.64 +0.2 146,110 + Atu6127 0.64 -0.3 146,110 + Atu6127 0.64 -0.1 146,117 - Atu6127 0.64 +0.0 146,117 - Atu6127 0.64 +4.3 146,118 - Atu6127 0.65 +1.8 146,118 - Atu6127 0.65 -0.7 146,154 + Atu6127 0.66 +0.0 146,180 + Atu6127 0.66 -1.3 146,180 + Atu6127 0.66 +0.7 146,199 - Atu6127 0.67 -0.0 146,230 + Atu6127 0.68 -0.2 146,376 + Atu6127 0.72 +1.1 146,382 - Atu6127 0.73 +0.1 146,421 + Atu6127 0.74 +0.2 146,429 - Atu6127 0.74 +1.3 146,456 - Atu6127 0.75 -0.8 146,531 - Atu6127 0.77 -0.3 146,677 - Atu6127 0.81 +2.0 146,677 - Atu6127 0.81 +0.3 146,716 + Atu6127 0.83 +2.5 146,755 + Atu6127 0.84 +2.7 146,794 + Atu6127 0.85 +1.4 146,794 + Atu6127 0.85 +0.8 146,794 + Atu6127 0.85 +0.6 146,794 + Atu6127 0.85 -0.1 146,941 + Atu6127 0.89 +1.5 146,941 + Atu6127 0.89 -0.0 146,947 - Atu6127 0.90 +0.0 147,050 - -0.4 147,077 + +1.3 147,077 + -0.3 147,082 + -0.6 147,141 + +0.2 147,164 + -0.3 147,212 - -0.0 147,236 - +1.7 147,288 + +0.7 147,301 + -0.4 147,301 + +1.3 147,331 + -0.4 147,339 - Atu6128 0.10 +2.7 147,339 - Atu6128 0.10 +0.3 147,469 + Atu6128 0.34 +0.0 147,515 + Atu6128 0.43 +1.5 147,525 + Atu6128 0.45 -0.5 147,535 - Atu6128 0.47 +0.7 147,535 - Atu6128 0.47 +1.3 147,536 + Atu6128 0.47 -0.9 147,627 + Atu6128 0.64 +0.6 147,651 + Atu6128 0.69 +1.5 147,673 + Atu6128 0.73 +0.5 147,749 + Atu6128 0.87 +1.5 147,757 - Atu6128 0.89 -0.8 147,768 - +1.5 147,768 - -0.6 147,783 - -0.6 147,796 + +0.1 147,803 + +0.6 147,804 - +1.5 147,806 - -0.5 147,806 - +2.0 147,835 + +0.6 147,902 + +1.0 148,005 - +0.5 148,023 - Atu6129 0.11 +0.5 148,049 - Atu6129 0.13 +0.2 148,101 - Atu6129 0.18 +1.6 148,101 - Atu6129 0.18 +1.0 148,141 + Atu6129 0.21 -0.2 148,141 + Atu6129 0.21 +0.6 148,149 - Atu6129 0.22 -0.4 148,198 - Atu6129 0.27 +0.1 148,223 + Atu6129 0.29 -0.0 148,274 + Atu6129 0.34 +1.2
Or see this region's nucleotide sequence