Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF663 and GFF664 overlap by 4 nucleotides GFF664 and GFF665 are separated by 44 nucleotides GFF665 and GFF666 are separated by 196 nucleotides
GFF663 - Imidazolonepropionase (EC 3.5.2.7), at 18,905 to 20,128
GFF663
GFF664 - Formiminoglutamase (EC 3.5.3.8), at 20,125 to 21,066
GFF664
GFF665 - Histidine utilization repressor, at 21,111 to 21,836
GFF665
GFF666 - Urocanate hydratase (EC 4.2.1.49), at 22,033 to 23,178
GFF666
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-1
0
1
2 at 19.444 kb on + strand, within GFF663 at 19.478 kb on + strand, within GFF663 at 19.614 kb on + strand, within GFF663 at 19.713 kb on - strand, within GFF663 at 20.067 kb on + strand at 20.068 kb on - strand at 20.068 kb on - strand at 20.068 kb on - strand at 20.072 kb on + strand at 20.073 kb on - strand at 20.073 kb on - strand at 20.094 kb on + strand at 20.178 kb on + strand at 20.179 kb on - strand at 20.180 kb on + strand at 20.180 kb on + strand at 20.181 kb on - strand at 20.181 kb on - strand at 20.581 kb on - strand, within GFF664 at 20.842 kb on - strand, within GFF664 at 20.855 kb on + strand, within GFF664 at 20.855 kb on + strand, within GFF664 at 21.114 kb on + strand at 21.114 kb on + strand at 21.115 kb on - strand at 21.316 kb on + strand, within GFF665 at 21.522 kb on + strand, within GFF665 at 21.600 kb on + strand, within GFF665 at 21.663 kb on - strand, within GFF665 at 21.710 kb on + strand, within GFF665 at 21.787 kb on + strand at 21.827 kb on + strand at 21.935 kb on - strand at 22.015 kb on + strand at 22.016 kb on - strand at 22.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 19,444 + GFF663 0.44 +0.3 19,478 + GFF663 0.47 +0.5 19,614 + GFF663 0.58 +1.0 19,713 - GFF663 0.66 +0.1 20,067 + +1.0 20,068 - +0.4 20,068 - +0.2 20,068 - +1.6 20,072 + +0.8 20,073 - +2.2 20,073 - +0.7 20,094 + +1.1 20,178 + +1.0 20,179 - +1.1 20,180 + +1.2 20,180 + +0.8 20,181 - +0.9 20,181 - +1.2 20,581 - GFF664 0.48 +0.8 20,842 - GFF664 0.76 +0.7 20,855 + GFF664 0.77 +0.4 20,855 + GFF664 0.77 +1.1 21,114 + +0.5 21,114 + +1.1 21,115 - +1.3 21,316 + GFF665 0.28 +0.2 21,522 + GFF665 0.57 +1.1 21,600 + GFF665 0.67 -0.2 21,663 - GFF665 0.76 +1.2 21,710 + GFF665 0.83 -1.3 21,787 + -0.7 21,827 + +0.2 21,935 - +1.5 22,015 + -0.3 22,016 - +1.2 22,048 + +0.6
Or see this region's nucleotide sequence