Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF574 and GFF575 are separated by 346 nucleotides GFF575 and GFF576 overlap by 4 nucleotides
GFF574 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 303,344 to 304,450
GFF574
GFF575 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 304,797 to 306,983
GFF575
GFF576 - Glycogen debranching enzyme (EC 3.2.1.-), at 306,980 to 308,956
GFF576
Position (kb)
304
305
306
307 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 304.490 kb on + strand at 304.645 kb on + strand at 304.645 kb on + strand at 304.645 kb on + strand at 304.662 kb on + strand at 304.672 kb on - strand at 304.682 kb on + strand at 304.696 kb on + strand at 304.696 kb on + strand at 304.826 kb on + strand at 304.827 kb on - strand at 304.898 kb on - strand at 304.961 kb on + strand at 304.962 kb on - strand at 305.111 kb on - strand, within GFF575 at 305.316 kb on - strand, within GFF575 at 305.316 kb on - strand, within GFF575 at 305.400 kb on - strand, within GFF575 at 305.532 kb on + strand, within GFF575 at 305.532 kb on + strand, within GFF575 at 305.546 kb on - strand, within GFF575 at 305.546 kb on - strand, within GFF575 at 305.564 kb on + strand, within GFF575 at 305.919 kb on + strand, within GFF575 at 305.919 kb on + strand, within GFF575 at 305.920 kb on - strand, within GFF575 at 305.925 kb on + strand, within GFF575 at 305.926 kb on - strand, within GFF575 at 305.926 kb on - strand, within GFF575 at 305.971 kb on - strand, within GFF575 at 306.023 kb on + strand, within GFF575 at 306.030 kb on + strand, within GFF575 at 306.119 kb on + strand, within GFF575 at 306.231 kb on + strand, within GFF575 at 306.333 kb on + strand, within GFF575 at 306.454 kb on - strand, within GFF575 at 306.454 kb on - strand, within GFF575 at 306.455 kb on + strand, within GFF575 at 306.456 kb on + strand, within GFF575 at 306.457 kb on - strand, within GFF575 at 306.624 kb on + strand, within GFF575 at 306.635 kb on + strand, within GFF575 at 306.636 kb on - strand, within GFF575 at 306.636 kb on - strand, within GFF575 at 306.767 kb on + strand at 306.929 kb on + strand at 307.025 kb on + strand at 307.026 kb on - strand at 307.026 kb on - strand at 307.026 kb on - strand at 307.077 kb on + strand at 307.077 kb on + strand at 307.290 kb on + strand, within GFF576 at 307.385 kb on + strand, within GFF576 at 307.386 kb on - strand, within GFF576 at 307.440 kb on - strand, within GFF576 at 307.466 kb on + strand, within GFF576 at 307.494 kb on + strand, within GFF576 at 307.505 kb on + strand, within GFF576 at 307.505 kb on + strand, within GFF576 at 307.642 kb on + strand, within GFF576 at 307.642 kb on + strand, within GFF576 at 307.643 kb on - strand, within GFF576 at 307.768 kb on - strand, within GFF576 at 307.770 kb on + strand, within GFF576 at 307.834 kb on + strand, within GFF576 at 307.838 kb on + strand, within GFF576 at 307.838 kb on + strand, within GFF576 at 307.838 kb on + strand, within GFF576 at 307.839 kb on - strand, within GFF576 at 307.839 kb on - strand, within GFF576 at 307.839 kb on - strand, within GFF576 at 307.841 kb on + strand, within GFF576
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 304,490 + +0.6 304,645 + -1.7 304,645 + +0.1 304,645 + +0.1 304,662 + -2.2 304,672 - -0.6 304,682 + -0.1 304,696 + +0.3 304,696 + -0.4 304,826 + -0.1 304,827 - +0.5 304,898 - +0.3 304,961 + -0.1 304,962 - +0.6 305,111 - GFF575 0.14 +0.3 305,316 - GFF575 0.24 +0.8 305,316 - GFF575 0.24 -0.0 305,400 - GFF575 0.28 -0.5 305,532 + GFF575 0.34 -0.7 305,532 + GFF575 0.34 -1.0 305,546 - GFF575 0.34 -0.4 305,546 - GFF575 0.34 -0.7 305,564 + GFF575 0.35 +0.3 305,919 + GFF575 0.51 -0.2 305,919 + GFF575 0.51 +0.6 305,920 - GFF575 0.51 +0.4 305,925 + GFF575 0.52 +0.6 305,926 - GFF575 0.52 +0.5 305,926 - GFF575 0.52 +0.4 305,971 - GFF575 0.54 +0.2 306,023 + GFF575 0.56 +1.0 306,030 + GFF575 0.56 -0.4 306,119 + GFF575 0.60 +0.5 306,231 + GFF575 0.66 +2.1 306,333 + GFF575 0.70 +0.5 306,454 - GFF575 0.76 +1.2 306,454 - GFF575 0.76 +0.4 306,455 + GFF575 0.76 +0.4 306,456 + GFF575 0.76 -0.1 306,457 - GFF575 0.76 +1.1 306,624 + GFF575 0.84 +0.5 306,635 + GFF575 0.84 -0.0 306,636 - GFF575 0.84 -1.0 306,636 - GFF575 0.84 -0.2 306,767 + +0.5 306,929 + -1.2 307,025 + +1.2 307,026 - +0.5 307,026 - +0.1 307,026 - -0.1 307,077 + +1.0 307,077 + +0.8 307,290 + GFF576 0.16 -0.2 307,385 + GFF576 0.20 +1.0 307,386 - GFF576 0.21 +0.3 307,440 - GFF576 0.23 -0.0 307,466 + GFF576 0.25 -0.0 307,494 + GFF576 0.26 +0.6 307,505 + GFF576 0.27 +0.4 307,505 + GFF576 0.27 +0.2 307,642 + GFF576 0.33 +0.1 307,642 + GFF576 0.33 -2.0 307,643 - GFF576 0.34 +0.2 307,768 - GFF576 0.40 -1.2 307,770 + GFF576 0.40 +0.0 307,834 + GFF576 0.43 +0.1 307,838 + GFF576 0.43 +0.1 307,838 + GFF576 0.43 +1.4 307,838 + GFF576 0.43 +0.1 307,839 - GFF576 0.43 +0.7 307,839 - GFF576 0.43 -0.3 307,839 - GFF576 0.43 +0.6 307,841 + GFF576 0.44 +0.3
Or see this region's nucleotide sequence