Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4777 and GFF4778 overlap by 4 nucleotides GFF4778 and GFF4779 are separated by 39 nucleotides GFF4779 and GFF4780 are separated by 4 nucleotides GFF4780 and GFF4781 overlap by 4 nucleotides
GFF4777 - Gluconate permease, at 85,332 to 86,828
GFF4777
GFF4778 - Nucleoside-diphosphate-sugar epimerases, at 86,825 to 87,775
GFF4778
GFF4779 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 87,815 to 88,591
GFF4779
GFF4780 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 88,596 to 89,234
GFF4780
GFF4781 - FIG00641944: hypothetical protein, at 89,231 to 90,493
GFF4781
Position (kb) 87
88
89 Strain fitness (log2 ratio) -2
-1
0
1 at 87.294 kb on + strand, within GFF4778 at 87.713 kb on + strand at 87.790 kb on + strand at 87.791 kb on - strand at 87.791 kb on - strand at 87.800 kb on + strand at 87.805 kb on + strand at 87.805 kb on + strand at 87.805 kb on + strand at 87.871 kb on - strand at 87.899 kb on + strand, within GFF4779 at 87.931 kb on - strand, within GFF4779 at 87.984 kb on - strand, within GFF4779 at 87.984 kb on - strand, within GFF4779 at 88.134 kb on + strand, within GFF4779 at 88.134 kb on + strand, within GFF4779 at 88.175 kb on - strand, within GFF4779 at 88.436 kb on - strand, within GFF4779 at 88.446 kb on - strand, within GFF4779 at 88.541 kb on - strand at 88.629 kb on + strand at 88.695 kb on - strand, within GFF4780 at 88.752 kb on + strand, within GFF4780 at 88.820 kb on - strand, within GFF4780 at 88.820 kb on - strand, within GFF4780 at 88.944 kb on + strand, within GFF4780 at 88.945 kb on - strand, within GFF4780 at 88.945 kb on - strand, within GFF4780 at 89.003 kb on + strand, within GFF4780 at 89.511 kb on + strand, within GFF4781 at 89.512 kb on - strand, within GFF4781
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 87,294 + GFF4778 0.49 +0.2 87,713 + -0.3 87,790 + +0.1 87,791 - -0.1 87,791 - +0.5 87,800 + +0.3 87,805 + +0.3 87,805 + +0.9 87,805 + +0.3 87,871 - +0.4 87,899 + GFF4779 0.11 +0.2 87,931 - GFF4779 0.15 -0.1 87,984 - GFF4779 0.22 +0.8 87,984 - GFF4779 0.22 -1.8 88,134 + GFF4779 0.41 +0.9 88,134 + GFF4779 0.41 +0.9 88,175 - GFF4779 0.46 +0.5 88,436 - GFF4779 0.80 +1.3 88,446 - GFF4779 0.81 +0.5 88,541 - +1.3 88,629 + -0.1 88,695 - GFF4780 0.15 +0.9 88,752 + GFF4780 0.24 -0.3 88,820 - GFF4780 0.35 +0.6 88,820 - GFF4780 0.35 -0.3 88,944 + GFF4780 0.54 +0.1 88,945 - GFF4780 0.55 +0.4 88,945 - GFF4780 0.55 +0.0 89,003 + GFF4780 0.64 -0.0 89,511 + GFF4781 0.22 +1.4 89,512 - GFF4781 0.22 +0.6
Or see this region's nucleotide sequence