Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4705 and GFF4706 are separated by 96 nucleotides GFF4706 and GFF4707 are separated by 145 nucleotides GFF4707 and GFF4708 overlap by 32 nucleotides GFF4708 and GFF4709 are separated by 97 nucleotides
GFF4705 - Nitric oxide reductase FlRd-NAD(+) reductase (EC 1.18.1.-), at 16,287 to 17,420
GFF4705
GFF4706 - [NiFe] hydrogenase metallocenter assembly protein HypF, at 17,517 to 19,757
GFF4706
GFF4707 - Fe-S-cluster-containing hydrogenase components 2, at 19,903 to 20,448
GFF4707
GFF4708 - hypothetical protein, at 20,417 to 20,551
GFF4708
GFF4709 - Putative periplasmic or exported protein, at 20,649 to 21,458
GFF4709
Position (kb)
17
18
19
20 Strain fitness (log2 ratio)
-1
0
1
2 at 16.521 kb on - strand, within GFF4705 at 16.572 kb on - strand, within GFF4705 at 16.649 kb on - strand, within GFF4705 at 16.664 kb on + strand, within GFF4705 at 16.754 kb on - strand, within GFF4705 at 16.852 kb on + strand, within GFF4705 at 16.853 kb on - strand, within GFF4705 at 16.949 kb on - strand, within GFF4705 at 16.959 kb on - strand, within GFF4705 at 16.962 kb on - strand, within GFF4705 at 16.996 kb on + strand, within GFF4705 at 17.060 kb on + strand, within GFF4705 at 17.061 kb on - strand, within GFF4705 at 17.064 kb on + strand, within GFF4705 at 17.069 kb on - strand, within GFF4705 at 17.091 kb on + strand, within GFF4705 at 17.209 kb on + strand, within GFF4705 at 17.294 kb on + strand, within GFF4705 at 17.295 kb on - strand, within GFF4705 at 17.295 kb on - strand, within GFF4705 at 17.298 kb on - strand, within GFF4705 at 17.316 kb on - strand at 17.351 kb on - strand at 17.922 kb on - strand, within GFF4706 at 17.922 kb on - strand, within GFF4706 at 18.114 kb on - strand, within GFF4706 at 18.336 kb on + strand, within GFF4706 at 18.337 kb on - strand, within GFF4706 at 18.628 kb on + strand, within GFF4706 at 18.909 kb on + strand, within GFF4706 at 18.913 kb on + strand, within GFF4706 at 18.914 kb on - strand, within GFF4706 at 18.930 kb on + strand, within GFF4706 at 19.308 kb on - strand, within GFF4706 at 19.574 kb on - strand at 19.637 kb on + strand at 19.760 kb on + strand at 19.760 kb on + strand at 19.816 kb on - strand at 19.885 kb on + strand at 20.020 kb on - strand, within GFF4707 at 20.126 kb on - strand, within GFF4707 at 20.319 kb on + strand, within GFF4707 at 20.485 kb on - strand, within GFF4708 at 20.487 kb on - strand, within GFF4708 at 20.487 kb on - strand, within GFF4708 at 20.751 kb on - strand, within GFF4709 at 20.754 kb on + strand, within GFF4709
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 16,521 - GFF4705 0.21 +0.5 16,572 - GFF4705 0.25 +0.6 16,649 - GFF4705 0.32 -0.2 16,664 + GFF4705 0.33 -0.5 16,754 - GFF4705 0.41 -0.3 16,852 + GFF4705 0.50 +0.4 16,853 - GFF4705 0.50 +1.5 16,949 - GFF4705 0.58 +0.2 16,959 - GFF4705 0.59 -0.3 16,962 - GFF4705 0.60 +0.2 16,996 + GFF4705 0.63 +0.9 17,060 + GFF4705 0.68 -0.9 17,061 - GFF4705 0.68 +0.2 17,064 + GFF4705 0.69 +1.7 17,069 - GFF4705 0.69 -0.2 17,091 + GFF4705 0.71 +0.7 17,209 + GFF4705 0.81 +1.2 17,294 + GFF4705 0.89 +1.1 17,295 - GFF4705 0.89 -0.3 17,295 - GFF4705 0.89 +0.3 17,298 - GFF4705 0.89 +0.7 17,316 - +1.5 17,351 - +0.4 17,922 - GFF4706 0.18 -0.1 17,922 - GFF4706 0.18 +0.5 18,114 - GFF4706 0.27 -0.2 18,336 + GFF4706 0.37 +0.6 18,337 - GFF4706 0.37 +1.9 18,628 + GFF4706 0.50 +0.5 18,909 + GFF4706 0.62 +1.6 18,913 + GFF4706 0.62 +1.0 18,914 - GFF4706 0.62 +0.5 18,930 + GFF4706 0.63 +0.2 19,308 - GFF4706 0.80 +0.6 19,574 - -0.2 19,637 + +0.3 19,760 + +0.2 19,760 + +0.5 19,816 - +1.1 19,885 + -0.1 20,020 - GFF4707 0.21 +0.7 20,126 - GFF4707 0.41 +0.1 20,319 + GFF4707 0.76 -0.6 20,485 - GFF4708 0.50 +0.4 20,487 - GFF4708 0.52 +0.2 20,487 - GFF4708 0.52 +0.1 20,751 - GFF4709 0.13 +0.8 20,754 + GFF4709 0.13 -0.1
Or see this region's nucleotide sequence