Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4605 and GFF4606 are separated by 250 nucleotides GFF4606 and GFF4607 overlap by 4 nucleotides GFF4607 and GFF4608 are separated by 68 nucleotides GFF4608 and GFF4609 are separated by 8 nucleotides
GFF4605 - hypothetical protein, at 396,519 to 396,632
GFF4605
GFF4606 - sensor histidine kinase, at 396,883 to 397,560
GFF4606
GFF4607 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 397,557 to 400,169
GFF4607
GFF4608 - Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 400,238 to 400,822
GFF4608
GFF4609 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 400,831 to 402,879
GFF4609
Position (kb) 397
398
399
400
401 Strain fitness (log2 ratio) -1
0
1
2 at 396.922 kb on - strand at 396.977 kb on + strand, within GFF4606 at 396.977 kb on + strand, within GFF4606 at 396.978 kb on - strand, within GFF4606 at 397.030 kb on + strand, within GFF4606 at 397.065 kb on + strand, within GFF4606 at 397.065 kb on + strand, within GFF4606 at 397.268 kb on + strand, within GFF4606 at 397.448 kb on - strand, within GFF4606 at 397.459 kb on + strand, within GFF4606 at 397.476 kb on - strand, within GFF4606 at 397.643 kb on + strand at 397.675 kb on - strand at 397.872 kb on - strand, within GFF4607 at 397.911 kb on + strand, within GFF4607 at 397.974 kb on + strand, within GFF4607 at 398.063 kb on + strand, within GFF4607 at 398.376 kb on + strand, within GFF4607 at 398.377 kb on - strand, within GFF4607 at 398.377 kb on - strand, within GFF4607 at 398.391 kb on - strand, within GFF4607 at 398.417 kb on - strand, within GFF4607 at 398.697 kb on + strand, within GFF4607 at 398.709 kb on - strand, within GFF4607 at 398.813 kb on - strand, within GFF4607 at 399.237 kb on - strand, within GFF4607 at 399.361 kb on - strand, within GFF4607 at 399.465 kb on - strand, within GFF4607 at 399.504 kb on + strand, within GFF4607 at 399.652 kb on + strand, within GFF4607 at 399.659 kb on + strand, within GFF4607 at 399.659 kb on + strand, within GFF4607 at 399.660 kb on - strand, within GFF4607 at 399.709 kb on - strand, within GFF4607 at 400.337 kb on - strand, within GFF4608 at 400.337 kb on - strand, within GFF4608 at 400.338 kb on + strand, within GFF4608 at 400.339 kb on - strand, within GFF4608 at 400.432 kb on + strand, within GFF4608 at 400.433 kb on - strand, within GFF4608 at 400.530 kb on - strand, within GFF4608 at 400.592 kb on - strand, within GFF4608 at 400.594 kb on - strand, within GFF4608 at 400.677 kb on - strand, within GFF4608 at 400.690 kb on + strand, within GFF4608 at 400.788 kb on - strand at 400.788 kb on - strand at 400.812 kb on - strand at 400.825 kb on + strand at 400.826 kb on - strand at 400.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 396,922 - -0.0 396,977 + GFF4606 0.14 +0.1 396,977 + GFF4606 0.14 -0.2 396,978 - GFF4606 0.14 +0.5 397,030 + GFF4606 0.22 +1.4 397,065 + GFF4606 0.27 +1.1 397,065 + GFF4606 0.27 +0.3 397,268 + GFF4606 0.57 +1.0 397,448 - GFF4606 0.83 +1.3 397,459 + GFF4606 0.85 +0.1 397,476 - GFF4606 0.87 +0.0 397,643 + +0.4 397,675 - +0.5 397,872 - GFF4607 0.12 +1.0 397,911 + GFF4607 0.14 -0.3 397,974 + GFF4607 0.16 -0.1 398,063 + GFF4607 0.19 -0.5 398,376 + GFF4607 0.31 +0.0 398,377 - GFF4607 0.31 +1.2 398,377 - GFF4607 0.31 -0.9 398,391 - GFF4607 0.32 +0.7 398,417 - GFF4607 0.33 +1.2 398,697 + GFF4607 0.44 +1.6 398,709 - GFF4607 0.44 +0.3 398,813 - GFF4607 0.48 +0.3 399,237 - GFF4607 0.64 +0.5 399,361 - GFF4607 0.69 +0.5 399,465 - GFF4607 0.73 +0.6 399,504 + GFF4607 0.75 +0.7 399,652 + GFF4607 0.80 +0.2 399,659 + GFF4607 0.80 +0.8 399,659 + GFF4607 0.80 +0.5 399,660 - GFF4607 0.80 +0.9 399,709 - GFF4607 0.82 -0.2 400,337 - GFF4608 0.17 +0.9 400,337 - GFF4608 0.17 +1.0 400,338 + GFF4608 0.17 +1.8 400,339 - GFF4608 0.17 +0.7 400,432 + GFF4608 0.33 -0.2 400,433 - GFF4608 0.33 +1.8 400,530 - GFF4608 0.50 +0.5 400,592 - GFF4608 0.61 +1.1 400,594 - GFF4608 0.61 +0.9 400,677 - GFF4608 0.75 +2.5 400,690 + GFF4608 0.77 +1.1 400,788 - -0.2 400,788 - +0.7 400,812 - +0.9 400,825 + +0.9 400,826 - +0.7 400,882 - +0.4
Or see this region's nucleotide sequence