Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4528 and GFF4529 overlap by 4 nucleotides GFF4529 and GFF4530 overlap by 4 nucleotides GFF4530 and GFF4531 are separated by 210 nucleotides GFF4531 and GFF4532 are separated by 9 nucleotides
GFF4528 - Citrate lyase beta chain (EC 4.1.3.6), at 315,582 to 316,490
GFF4528
GFF4529 - Citrate lyase gamma chain, acyl carrier protein (EC 4.1.3.6), at 316,487 to 316,783
GFF4529
GFF4530 - [Citrate [pro-3S]-lyase] ligase (EC 6.2.1.22), at 316,780 to 317,856
GFF4530
GFF4531 - FIG01046674: hypothetical protein, at 318,067 to 318,231
GFF4531
GFF4532 - Sensor kinase CitA, DpiB (EC 2.7.3.-), at 318,241 to 319,902
GFF4532
Position (kb) 316
317
318 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 315.806 kb on + strand, within GFF4528 at 315.806 kb on + strand, within GFF4528 at 315.807 kb on - strand, within GFF4528 at 315.807 kb on - strand, within GFF4528 at 315.992 kb on - strand, within GFF4528 at 315.992 kb on - strand, within GFF4528 at 316.315 kb on - strand, within GFF4528 at 316.395 kb on - strand, within GFF4528 at 316.442 kb on + strand at 316.443 kb on - strand at 316.465 kb on - strand at 316.766 kb on + strand at 317.045 kb on + strand, within GFF4530 at 317.178 kb on + strand, within GFF4530 at 317.186 kb on + strand, within GFF4530 at 317.212 kb on - strand, within GFF4530 at 317.307 kb on + strand, within GFF4530 at 317.308 kb on - strand, within GFF4530 at 317.409 kb on + strand, within GFF4530 at 317.410 kb on - strand, within GFF4530 at 317.410 kb on - strand, within GFF4530 at 317.410 kb on - strand, within GFF4530 at 317.492 kb on - strand, within GFF4530 at 317.556 kb on - strand, within GFF4530 at 318.358 kb on + strand at 318.529 kb on + strand, within GFF4532 at 318.641 kb on - strand, within GFF4532 at 318.694 kb on - strand, within GFF4532 at 318.751 kb on - strand, within GFF4532 at 318.822 kb on - strand, within GFF4532
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 315,806 + GFF4528 0.25 +1.0 315,806 + GFF4528 0.25 +1.0 315,807 - GFF4528 0.25 +0.1 315,807 - GFF4528 0.25 +0.4 315,992 - GFF4528 0.45 -0.3 315,992 - GFF4528 0.45 +0.3 316,315 - GFF4528 0.81 +0.9 316,395 - GFF4528 0.89 +0.7 316,442 + +1.0 316,443 - +0.3 316,465 - +0.9 316,766 + -1.1 317,045 + GFF4530 0.25 -1.1 317,178 + GFF4530 0.37 -0.7 317,186 + GFF4530 0.38 +0.2 317,212 - GFF4530 0.40 +0.2 317,307 + GFF4530 0.49 -0.1 317,308 - GFF4530 0.49 -0.1 317,409 + GFF4530 0.58 -0.8 317,410 - GFF4530 0.58 -0.4 317,410 - GFF4530 0.58 -3.5 317,410 - GFF4530 0.58 +1.5 317,492 - GFF4530 0.66 +0.1 317,556 - GFF4530 0.72 +0.2 318,358 + +0.4 318,529 + GFF4532 0.17 +0.2 318,641 - GFF4532 0.24 -0.7 318,694 - GFF4532 0.27 -0.3 318,751 - GFF4532 0.31 -0.2 318,822 - GFF4532 0.35 -2.2
Or see this region's nucleotide sequence