Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4430 and GFF4431 are separated by 79 nucleotides GFF4431 and GFF4432 are separated by 10 nucleotides GFF4432 and GFF4433 are separated by 21 nucleotides
GFF4430 - Glycerate kinase (EC 2.7.1.31), at 216,913 to 218,061
GFF4430
GFF4431 - Ureidoglycine aminohydrolase, at 218,141 to 218,926
GFF4431
GFF4432 - Allantoate amidohydrolase (EC 3.5.3.9), at 218,937 to 220,172
GFF4432
GFF4433 - Ureidoglycolate dehydrogenase (EC 1.1.1.154), at 220,194 to 221,243
GFF4433
Position (kb) 218
219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 218.013 kb on + strand at 218.105 kb on - strand at 218.176 kb on - strand at 218.181 kb on + strand at 218.248 kb on - strand, within GFF4431 at 218.314 kb on - strand, within GFF4431 at 218.347 kb on - strand, within GFF4431 at 218.381 kb on - strand, within GFF4431 at 218.386 kb on + strand, within GFF4431 at 218.387 kb on - strand, within GFF4431 at 218.387 kb on - strand, within GFF4431 at 218.452 kb on - strand, within GFF4431 at 218.519 kb on - strand, within GFF4431 at 218.519 kb on - strand, within GFF4431 at 218.519 kb on - strand, within GFF4431 at 218.531 kb on - strand, within GFF4431 at 218.541 kb on - strand, within GFF4431 at 218.598 kb on + strand, within GFF4431 at 218.645 kb on - strand, within GFF4431 at 218.729 kb on + strand, within GFF4431 at 218.729 kb on + strand, within GFF4431 at 219.002 kb on + strand at 219.003 kb on - strand at 219.003 kb on - strand at 219.012 kb on - strand at 219.036 kb on - strand at 219.323 kb on + strand, within GFF4432 at 219.345 kb on + strand, within GFF4432 at 219.345 kb on + strand, within GFF4432 at 219.346 kb on - strand, within GFF4432 at 219.346 kb on - strand, within GFF4432 at 219.390 kb on + strand, within GFF4432 at 219.440 kb on + strand, within GFF4432 at 219.662 kb on + strand, within GFF4432 at 219.663 kb on - strand, within GFF4432 at 219.724 kb on - strand, within GFF4432 at 219.986 kb on - strand, within GFF4432 at 220.410 kb on + strand, within GFF4433 at 220.411 kb on - strand, within GFF4433 at 220.593 kb on + strand, within GFF4433 at 220.731 kb on - strand, within GFF4433 at 220.775 kb on + strand, within GFF4433 at 221.090 kb on - strand, within GFF4433 at 221.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 218,013 + -0.1 218,105 - -0.2 218,176 - +0.5 218,181 + -1.5 218,248 - GFF4431 0.14 +0.3 218,314 - GFF4431 0.22 +0.2 218,347 - GFF4431 0.26 +0.2 218,381 - GFF4431 0.31 -0.5 218,386 + GFF4431 0.31 +0.3 218,387 - GFF4431 0.31 -0.5 218,387 - GFF4431 0.31 -0.5 218,452 - GFF4431 0.40 -0.6 218,519 - GFF4431 0.48 +0.2 218,519 - GFF4431 0.48 -0.1 218,519 - GFF4431 0.48 +0.1 218,531 - GFF4431 0.50 +0.2 218,541 - GFF4431 0.51 +0.3 218,598 + GFF4431 0.58 -0.1 218,645 - GFF4431 0.64 +0.0 218,729 + GFF4431 0.75 +0.0 218,729 + GFF4431 0.75 -0.1 219,002 + -0.2 219,003 - -0.3 219,003 - -0.6 219,012 - -1.4 219,036 - +1.7 219,323 + GFF4432 0.31 -0.6 219,345 + GFF4432 0.33 -0.2 219,345 + GFF4432 0.33 -0.3 219,346 - GFF4432 0.33 +0.1 219,346 - GFF4432 0.33 -0.8 219,390 + GFF4432 0.37 -3.6 219,440 + GFF4432 0.41 -0.4 219,662 + GFF4432 0.59 -0.8 219,663 - GFF4432 0.59 -0.6 219,724 - GFF4432 0.64 -0.7 219,986 - GFF4432 0.85 +0.1 220,410 + GFF4433 0.21 +0.3 220,411 - GFF4433 0.21 -0.3 220,593 + GFF4433 0.38 -2.1 220,731 - GFF4433 0.51 +0.6 220,775 + GFF4433 0.55 -0.9 221,090 - GFF4433 0.85 -0.2 221,140 + +0.7
Or see this region's nucleotide sequence