Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4423

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4421 and GFF4422 are separated by 78 nucleotidesGFF4422 and GFF4423 are separated by 84 nucleotidesGFF4423 and GFF4424 are separated by 12 nucleotidesGFF4424 and GFF4425 are separated by 100 nucleotides GFF4421 - Ureidoglycolate hydrolase (EC 3.5.3.19), at 206,217 to 206,699 GFF4421 GFF4422 - Negative regulator of allantoin and glyoxylate utilization operons, at 206,778 to 207,596 GFF4422 GFF4423 - Glyoxylate carboligase (EC 4.1.1.47), at 207,681 to 209,462 GFF4423 GFF4424 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 209,475 to 210,251 GFF4424 GFF4425 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 210,352 to 211,230 GFF4425 Position (kb) 207 208 209 210Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 206.720 kb on - strandat 206.731 kb on - strandat 206.731 kb on - strandat 206.774 kb on - strandat 206.777 kb on + strandat 206.789 kb on - strandat 206.875 kb on - strand, within GFF4422at 207.036 kb on - strand, within GFF4422at 207.036 kb on - strand, within GFF4422at 207.036 kb on - strand, within GFF4422at 207.053 kb on + strand, within GFF4422at 207.060 kb on - strand, within GFF4422at 207.092 kb on + strand, within GFF4422at 207.104 kb on + strand, within GFF4422at 207.105 kb on - strand, within GFF4422at 207.229 kb on + strand, within GFF4422at 207.229 kb on + strand, within GFF4422at 207.347 kb on + strand, within GFF4422at 207.444 kb on + strand, within GFF4422at 207.444 kb on + strand, within GFF4422at 207.444 kb on + strand, within GFF4422at 207.445 kb on - strand, within GFF4422at 207.445 kb on - strand, within GFF4422at 207.714 kb on + strandat 207.714 kb on + strandat 207.714 kb on + strandat 207.715 kb on - strandat 207.780 kb on + strandat 207.780 kb on + strandat 207.781 kb on - strandat 207.817 kb on + strandat 207.818 kb on - strandat 207.818 kb on - strandat 207.861 kb on + strand, within GFF4423at 208.212 kb on + strand, within GFF4423at 208.212 kb on + strand, within GFF4423at 208.213 kb on - strand, within GFF4423at 208.231 kb on - strand, within GFF4423at 208.232 kb on + strand, within GFF4423at 208.628 kb on - strand, within GFF4423at 208.746 kb on - strand, within GFF4423at 208.763 kb on + strand, within GFF4423at 208.846 kb on - strand, within GFF4423at 209.076 kb on + strand, within GFF4423at 209.076 kb on + strand, within GFF4423at 209.078 kb on + strand, within GFF4423at 209.104 kb on - strand, within GFF4423at 209.148 kb on + strand, within GFF4423at 209.156 kb on - strand, within GFF4423at 209.175 kb on - strand, within GFF4423at 209.215 kb on + strand, within GFF4423at 209.215 kb on + strand, within GFF4423at 209.270 kb on + strand, within GFF4423at 209.454 kb on + strandat 209.898 kb on - strand, within GFF4424at 209.988 kb on - strand, within GFF4424at 210.008 kb on + strand, within GFF4424at 210.009 kb on - strand, within GFF4424at 210.195 kb on + strandat 210.337 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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206,720 - +0.0
206,731 - +0.1
206,731 - +0.2
206,774 - +2.3
206,777 + -1.0
206,789 - -1.2
206,875 - GFF4422 0.12 -0.3
207,036 - GFF4422 0.32 +1.3
207,036 - GFF4422 0.32 -0.4
207,036 - GFF4422 0.32 +0.8
207,053 + GFF4422 0.34 +0.2
207,060 - GFF4422 0.34 -0.8
207,092 + GFF4422 0.38 -0.5
207,104 + GFF4422 0.40 -0.3
207,105 - GFF4422 0.40 -0.0
207,229 + GFF4422 0.55 -0.5
207,229 + GFF4422 0.55 +0.5
207,347 + GFF4422 0.69 -0.6
207,444 + GFF4422 0.81 -0.7
207,444 + GFF4422 0.81 -0.0
207,444 + GFF4422 0.81 -0.4
207,445 - GFF4422 0.81 -0.7
207,445 - GFF4422 0.81 +0.3
207,714 + +0.2
207,714 + -1.3
207,714 + -0.5
207,715 - +1.5
207,780 + +1.2
207,780 + -0.2
207,781 - -0.0
207,817 + -0.2
207,818 - -1.4
207,818 - +1.2
207,861 + GFF4423 0.10 +0.1
208,212 + GFF4423 0.30 +0.2
208,212 + GFF4423 0.30 -0.2
208,213 - GFF4423 0.30 -0.2
208,231 - GFF4423 0.31 -1.5
208,232 + GFF4423 0.31 -0.5
208,628 - GFF4423 0.53 -0.4
208,746 - GFF4423 0.60 -0.2
208,763 + GFF4423 0.61 -1.0
208,846 - GFF4423 0.65 -0.5
209,076 + GFF4423 0.78 +1.0
209,076 + GFF4423 0.78 -0.5
209,078 + GFF4423 0.78 -0.0
209,104 - GFF4423 0.80 +0.9
209,148 + GFF4423 0.82 -3.2
209,156 - GFF4423 0.83 -0.1
209,175 - GFF4423 0.84 -0.2
209,215 + GFF4423 0.86 -0.2
209,215 + GFF4423 0.86 -0.7
209,270 + GFF4423 0.89 -0.5
209,454 + -0.4
209,898 - GFF4424 0.54 -0.2
209,988 - GFF4424 0.66 -1.0
210,008 + GFF4424 0.69 +0.0
210,009 - GFF4424 0.69 -0.3
210,195 + +0.3
210,337 + -0.3

Or see this region's nucleotide sequence