Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF423

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF422 and GFF423 are separated by 168 nucleotidesGFF423 and GFF424 are separated by 117 nucleotides GFF422 - Putative sugar kinase, at 142,517 to 143,398 GFF422 GFF423 - Aldehyde dehydrogenase B (EC 1.2.1.22), at 143,567 to 145,105 GFF423 GFF424 - Putative glycosyl hydrolase of unknown function (DUF1680), at 145,223 to 147,178 GFF424 Position (kb) 143 144 145 146Strain fitness (log2 ratio) -1 0 1 2 3at 142.728 kb on + strand, within GFF422at 142.869 kb on - strand, within GFF422at 142.891 kb on + strand, within GFF422at 142.892 kb on - strand, within GFF422at 142.898 kb on - strand, within GFF422at 142.982 kb on - strand, within GFF422at 143.342 kb on - strandat 143.389 kb on - strandat 143.398 kb on + strandat 143.398 kb on + strandat 143.399 kb on - strandat 143.431 kb on + strandat 143.432 kb on - strandat 143.435 kb on - strandat 143.471 kb on - strandat 143.543 kb on + strandat 143.544 kb on - strandat 143.554 kb on + strandat 143.628 kb on + strandat 143.650 kb on + strandat 143.783 kb on - strand, within GFF423at 143.806 kb on + strand, within GFF423at 143.814 kb on + strand, within GFF423at 143.938 kb on - strand, within GFF423at 143.977 kb on + strand, within GFF423at 143.978 kb on - strand, within GFF423at 143.978 kb on - strand, within GFF423at 143.978 kb on - strand, within GFF423at 143.980 kb on - strand, within GFF423at 144.336 kb on - strand, within GFF423at 144.392 kb on - strand, within GFF423at 144.392 kb on - strand, within GFF423at 144.470 kb on - strand, within GFF423at 144.487 kb on + strand, within GFF423at 144.487 kb on + strand, within GFF423at 144.488 kb on - strand, within GFF423at 144.525 kb on - strand, within GFF423at 144.595 kb on + strand, within GFF423at 144.647 kb on + strand, within GFF423at 144.648 kb on - strand, within GFF423at 144.649 kb on + strand, within GFF423at 144.721 kb on + strand, within GFF423at 144.734 kb on + strand, within GFF423at 144.746 kb on - strand, within GFF423at 144.769 kb on + strand, within GFF423at 144.824 kb on - strand, within GFF423at 144.960 kb on - strandat 144.998 kb on - strandat 145.167 kb on - strandat 145.333 kb on - strandat 145.403 kb on - strandat 145.466 kb on - strand, within GFF424at 145.567 kb on - strand, within GFF424at 145.718 kb on + strand, within GFF424at 145.785 kb on + strand, within GFF424at 145.851 kb on + strand, within GFF424at 145.852 kb on - strand, within GFF424at 145.852 kb on - strand, within GFF424at 145.852 kb on - strand, within GFF424at 145.855 kb on + strand, within GFF424at 145.856 kb on - strand, within GFF424at 145.863 kb on + strand, within GFF424at 145.864 kb on - strand, within GFF424at 145.893 kb on + strand, within GFF424at 145.894 kb on - strand, within GFF424at 146.034 kb on + strand, within GFF424at 146.062 kb on + strand, within GFF424at 146.063 kb on - strand, within GFF424

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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142,728 + GFF422 0.24 +0.6
142,869 - GFF422 0.40 +0.6
142,891 + GFF422 0.42 +3.6
142,892 - GFF422 0.43 +0.1
142,898 - GFF422 0.43 +1.2
142,982 - GFF422 0.53 -0.4
143,342 - +1.2
143,389 - +0.4
143,398 + -0.1
143,398 + +0.4
143,399 - -1.1
143,431 + +0.2
143,432 - +0.1
143,435 - +0.3
143,471 - +0.2
143,543 + +0.3
143,544 - -1.0
143,554 + +0.2
143,628 + +0.4
143,650 + +0.6
143,783 - GFF423 0.14 -0.0
143,806 + GFF423 0.16 +0.6
143,814 + GFF423 0.16 +0.2
143,938 - GFF423 0.24 +0.2
143,977 + GFF423 0.27 -0.2
143,978 - GFF423 0.27 +0.4
143,978 - GFF423 0.27 +0.5
143,978 - GFF423 0.27 +0.8
143,980 - GFF423 0.27 +0.6
144,336 - GFF423 0.50 +0.4
144,392 - GFF423 0.54 +0.0
144,392 - GFF423 0.54 -0.0
144,470 - GFF423 0.59 +0.2
144,487 + GFF423 0.60 -0.9
144,487 + GFF423 0.60 -0.4
144,488 - GFF423 0.60 +0.2
144,525 - GFF423 0.62 -0.1
144,595 + GFF423 0.67 +0.2
144,647 + GFF423 0.70 +1.2
144,648 - GFF423 0.70 +0.5
144,649 + GFF423 0.70 +0.3
144,721 + GFF423 0.75 +0.9
144,734 + GFF423 0.76 -1.0
144,746 - GFF423 0.77 +0.4
144,769 + GFF423 0.78 +0.6
144,824 - GFF423 0.82 +0.5
144,960 - -1.2
144,998 - +0.2
145,167 - +1.2
145,333 - +0.6
145,403 - +1.2
145,466 - GFF424 0.12 +0.3
145,567 - GFF424 0.18 +0.1
145,718 + GFF424 0.25 +0.0
145,785 + GFF424 0.29 +0.2
145,851 + GFF424 0.32 +0.2
145,852 - GFF424 0.32 +0.3
145,852 - GFF424 0.32 +0.7
145,852 - GFF424 0.32 -0.5
145,855 + GFF424 0.32 +0.4
145,856 - GFF424 0.32 -0.9
145,863 + GFF424 0.33 +0.1
145,864 - GFF424 0.33 -0.2
145,893 + GFF424 0.34 -0.1
145,894 - GFF424 0.34 -0.5
146,034 + GFF424 0.41 +0.4
146,062 + GFF424 0.43 -0.4
146,063 - GFF424 0.43 -0.4

Or see this region's nucleotide sequence