Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3964 and GFF3965 are separated by 8 nucleotides GFF3965 and GFF3966 overlap by 4 nucleotides GFF3966 and GFF3967 overlap by 4 nucleotides GFF3967 and GFF3968 overlap by 4 nucleotides
GFF3964 - Transmembrane component CbiQ of energizing module of cobalt ECF transporter, at 242,153 to 242,830
GFF3964
GFF3965 - ATPase component CbiO of energizing module of cobalt ECF transporter, at 242,839 to 243,654
GFF3965
GFF3966 - Cobyric acid synthase (EC 6.3.5.10), at 243,651 to 245,171
GFF3966
GFF3967 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 245,168 to 245,713
GFF3967
GFF3968 - Cobalamin synthase (EC 2.7.8.26), at 245,710 to 246,453
GFF3968
Position (kb) 243
244
245
246 Strain fitness (log2 ratio) -2
-1
0
1
2 at 242.828 kb on + strand at 242.835 kb on + strand at 242.838 kb on + strand at 242.839 kb on - strand at 242.889 kb on + strand at 242.897 kb on + strand at 242.970 kb on + strand, within GFF3965 at 243.141 kb on + strand, within GFF3965 at 243.307 kb on + strand, within GFF3965 at 243.311 kb on + strand, within GFF3965 at 243.312 kb on - strand, within GFF3965 at 243.312 kb on - strand, within GFF3965 at 243.312 kb on - strand, within GFF3965 at 243.408 kb on + strand, within GFF3965 at 243.409 kb on - strand, within GFF3965 at 243.409 kb on - strand, within GFF3965 at 243.432 kb on + strand, within GFF3965 at 243.457 kb on + strand, within GFF3965 at 243.461 kb on + strand, within GFF3965 at 243.665 kb on + strand at 243.719 kb on - strand at 243.749 kb on + strand at 243.773 kb on + strand at 243.783 kb on - strand at 243.969 kb on + strand, within GFF3966 at 243.972 kb on - strand, within GFF3966 at 244.005 kb on + strand, within GFF3966 at 244.006 kb on - strand, within GFF3966 at 244.027 kb on - strand, within GFF3966 at 244.034 kb on + strand, within GFF3966 at 244.034 kb on + strand, within GFF3966 at 244.034 kb on + strand, within GFF3966 at 244.219 kb on + strand, within GFF3966 at 244.233 kb on - strand, within GFF3966 at 244.266 kb on + strand, within GFF3966 at 244.267 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.454 kb on + strand, within GFF3966 at 244.491 kb on + strand, within GFF3966 at 244.728 kb on - strand, within GFF3966 at 245.253 kb on - strand, within GFF3967 at 245.253 kb on - strand, within GFF3967 at 245.254 kb on + strand, within GFF3967 at 245.309 kb on - strand, within GFF3967 at 245.413 kb on + strand, within GFF3967 at 245.413 kb on + strand, within GFF3967 at 245.414 kb on - strand, within GFF3967 at 245.559 kb on + strand, within GFF3967 at 245.849 kb on + strand, within GFF3968
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 242,828 + +0.3 242,835 + +0.1 242,838 + +0.5 242,839 - -0.3 242,889 + -1.1 242,897 + -0.5 242,970 + GFF3965 0.16 -1.1 243,141 + GFF3965 0.37 +2.2 243,307 + GFF3965 0.57 -2.2 243,311 + GFF3965 0.58 +1.7 243,312 - GFF3965 0.58 -1.2 243,312 - GFF3965 0.58 -1.0 243,312 - GFF3965 0.58 -0.8 243,408 + GFF3965 0.70 -0.2 243,409 - GFF3965 0.70 -0.0 243,409 - GFF3965 0.70 +0.3 243,432 + GFF3965 0.73 +0.3 243,457 + GFF3965 0.76 +0.4 243,461 + GFF3965 0.76 +0.5 243,665 + -0.2 243,719 - -0.8 243,749 + +0.6 243,773 + -0.9 243,783 - +1.2 243,969 + GFF3966 0.21 -0.8 243,972 - GFF3966 0.21 +0.3 244,005 + GFF3966 0.23 +0.1 244,006 - GFF3966 0.23 -0.3 244,027 - GFF3966 0.25 +0.2 244,034 + GFF3966 0.25 +0.4 244,034 + GFF3966 0.25 +1.1 244,034 + GFF3966 0.25 +0.1 244,219 + GFF3966 0.37 +1.4 244,233 - GFF3966 0.38 +0.5 244,266 + GFF3966 0.40 -0.1 244,267 - GFF3966 0.40 +0.2 244,434 - GFF3966 0.51 +0.6 244,434 - GFF3966 0.51 +1.1 244,434 - GFF3966 0.51 -0.5 244,434 - GFF3966 0.51 +0.4 244,454 + GFF3966 0.53 +0.9 244,491 + GFF3966 0.55 +0.0 244,728 - GFF3966 0.71 +0.1 245,253 - GFF3967 0.16 +0.1 245,253 - GFF3967 0.16 -0.2 245,254 + GFF3967 0.16 +1.4 245,309 - GFF3967 0.26 +0.8 245,413 + GFF3967 0.45 +0.3 245,413 + GFF3967 0.45 +0.3 245,414 - GFF3967 0.45 +0.6 245,559 + GFF3967 0.72 +0.4 245,849 + GFF3968 0.19 +0.4
Or see this region's nucleotide sequence