Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3926 and GFF3927 are separated by 33 nucleotides GFF3927 and GFF3928 are separated by 93 nucleotides GFF3928 and GFF3929 overlap by 16 nucleotides GFF3929 and GFF3930 are separated by 16 nucleotides GFF3930 and GFF3931 overlap by 1 nucleotides
GFF3926 - UPF0265 protein YeeX, at 210,870 to 211,205
GFF3926
GFF3927 - Threonine kinase in B12 biosynthesis, at 211,239 to 212,105
GFF3927
GFF3928 - Propionate kinase, propanediol utilization (EC 2.7.2.1), at 212,199 to 213,413
GFF3928
GFF3929 - Propanediol utilization protein PduV, at 213,398 to 213,838
GFF3929
GFF3930 - Propanediol utilization polyhedral body protein PduU, at 213,855 to 214,205
GFF3930
GFF3931 - Propanediol utilization polyhedral body protein PduT, at 214,205 to 214,759
GFF3931
Position (kb) 212
213
214 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 211.212 kb on - strand at 211.227 kb on + strand at 211.323 kb on - strand at 211.340 kb on + strand, within GFF3927 at 211.386 kb on - strand, within GFF3927 at 211.406 kb on - strand, within GFF3927 at 211.459 kb on - strand, within GFF3927 at 211.527 kb on - strand, within GFF3927 at 211.717 kb on - strand, within GFF3927 at 211.832 kb on + strand, within GFF3927 at 211.901 kb on + strand, within GFF3927 at 212.002 kb on - strand, within GFF3927 at 212.109 kb on - strand at 212.141 kb on + strand at 212.142 kb on - strand at 212.192 kb on - strand at 212.266 kb on - strand at 212.436 kb on + strand, within GFF3928 at 212.468 kb on + strand, within GFF3928 at 212.468 kb on + strand, within GFF3928 at 212.470 kb on + strand, within GFF3928 at 212.470 kb on + strand, within GFF3928 at 212.471 kb on - strand, within GFF3928 at 212.614 kb on - strand, within GFF3928 at 212.616 kb on + strand, within GFF3928 at 212.781 kb on - strand, within GFF3928 at 212.860 kb on + strand, within GFF3928 at 212.860 kb on + strand, within GFF3928 at 213.278 kb on + strand, within GFF3928 at 213.279 kb on - strand, within GFF3928 at 213.508 kb on + strand, within GFF3929 at 213.532 kb on + strand, within GFF3929 at 213.691 kb on - strand, within GFF3929 at 213.712 kb on - strand, within GFF3929 at 213.769 kb on - strand, within GFF3929 at 213.857 kb on - strand at 214.165 kb on + strand, within GFF3930 at 214.166 kb on - strand, within GFF3930 at 214.351 kb on - strand, within GFF3931 at 214.357 kb on + strand, within GFF3931 at 214.358 kb on - strand, within GFF3931 at 214.358 kb on - strand, within GFF3931 at 214.379 kb on - strand, within GFF3931
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 211,212 - +2.2 211,227 + -1.0 211,323 - -0.8 211,340 + GFF3927 0.12 -1.6 211,386 - GFF3927 0.17 +0.6 211,406 - GFF3927 0.19 -1.2 211,459 - GFF3927 0.25 -0.9 211,527 - GFF3927 0.33 -0.2 211,717 - GFF3927 0.55 +0.1 211,832 + GFF3927 0.68 +0.2 211,901 + GFF3927 0.76 -0.9 212,002 - GFF3927 0.88 +0.7 212,109 - -0.0 212,141 + +0.0 212,142 - +0.6 212,192 - -0.5 212,266 - -0.3 212,436 + GFF3928 0.20 -2.3 212,468 + GFF3928 0.22 -0.6 212,468 + GFF3928 0.22 -0.3 212,470 + GFF3928 0.22 -1.6 212,470 + GFF3928 0.22 -3.2 212,471 - GFF3928 0.22 -0.6 212,614 - GFF3928 0.34 -1.4 212,616 + GFF3928 0.34 -0.3 212,781 - GFF3928 0.48 -1.1 212,860 + GFF3928 0.54 -0.7 212,860 + GFF3928 0.54 -0.0 213,278 + GFF3928 0.89 +0.2 213,279 - GFF3928 0.89 -0.9 213,508 + GFF3929 0.25 -0.3 213,532 + GFF3929 0.30 -0.4 213,691 - GFF3929 0.66 -0.1 213,712 - GFF3929 0.71 -0.1 213,769 - GFF3929 0.84 -0.2 213,857 - +1.0 214,165 + GFF3930 0.88 +0.6 214,166 - GFF3930 0.89 +0.0 214,351 - GFF3931 0.26 -0.2 214,357 + GFF3931 0.27 -1.8 214,358 - GFF3931 0.28 +0.4 214,358 - GFF3931 0.28 -0.2 214,379 - GFF3931 0.31 -0.8
Or see this region's nucleotide sequence