Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3882 and GFF3883 are separated by 21 nucleotides GFF3883 and GFF3884 overlap by 4 nucleotides
GFF3882 - Lipopolysaccharide biosynthesis protein WzxC, at 159,381 to 160,892
GFF3882
GFF3883 - Colanic acid biosysnthesis protein WcaK, at 160,914 to 162,194
GFF3883
GFF3884 - Colanic acid biosynthesis glycosyl transferase WcaL, at 162,191 to 163,411
GFF3884
Position (kb) 160
161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 160.074 kb on - strand, within GFF3882 at 160.084 kb on - strand, within GFF3882 at 160.150 kb on - strand, within GFF3882 at 160.152 kb on - strand, within GFF3882 at 160.162 kb on - strand, within GFF3882 at 160.194 kb on - strand, within GFF3882 at 160.273 kb on - strand, within GFF3882 at 160.273 kb on - strand, within GFF3882 at 160.275 kb on - strand, within GFF3882 at 160.485 kb on - strand, within GFF3882 at 160.485 kb on - strand, within GFF3882 at 160.654 kb on - strand, within GFF3882 at 160.656 kb on - strand, within GFF3882 at 160.741 kb on + strand at 160.773 kb on - strand at 160.902 kb on - strand at 161.266 kb on - strand, within GFF3883 at 161.368 kb on - strand, within GFF3883 at 161.577 kb on + strand, within GFF3883 at 161.765 kb on + strand, within GFF3883 at 161.818 kb on - strand, within GFF3883 at 161.973 kb on + strand, within GFF3883 at 161.982 kb on - strand, within GFF3883 at 162.007 kb on + strand, within GFF3883 at 162.007 kb on + strand, within GFF3883 at 162.266 kb on - strand at 162.322 kb on + strand, within GFF3884 at 162.322 kb on + strand, within GFF3884 at 162.345 kb on - strand, within GFF3884 at 162.422 kb on - strand, within GFF3884 at 162.557 kb on - strand, within GFF3884 at 162.557 kb on - strand, within GFF3884 at 162.653 kb on - strand, within GFF3884 at 162.658 kb on + strand, within GFF3884 at 163.139 kb on - strand, within GFF3884 at 163.185 kb on + strand, within GFF3884 at 163.189 kb on + strand, within GFF3884
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 160,074 - GFF3882 0.46 +1.0 160,084 - GFF3882 0.46 -1.1 160,150 - GFF3882 0.51 +0.1 160,152 - GFF3882 0.51 -1.1 160,162 - GFF3882 0.52 -0.3 160,194 - GFF3882 0.54 -1.1 160,273 - GFF3882 0.59 +1.4 160,273 - GFF3882 0.59 -0.4 160,275 - GFF3882 0.59 +0.0 160,485 - GFF3882 0.73 -0.6 160,485 - GFF3882 0.73 -0.9 160,654 - GFF3882 0.84 -0.5 160,656 - GFF3882 0.84 -0.8 160,741 + -0.9 160,773 - +0.0 160,902 - -0.6 161,266 - GFF3883 0.27 +0.1 161,368 - GFF3883 0.35 -0.8 161,577 + GFF3883 0.52 -0.6 161,765 + GFF3883 0.66 -1.2 161,818 - GFF3883 0.71 +1.0 161,973 + GFF3883 0.83 -1.1 161,982 - GFF3883 0.83 -0.8 162,007 + GFF3883 0.85 -0.6 162,007 + GFF3883 0.85 +0.2 162,266 - -0.4 162,322 + GFF3884 0.11 -0.9 162,322 + GFF3884 0.11 -1.0 162,345 - GFF3884 0.13 +1.1 162,422 - GFF3884 0.19 -3.1 162,557 - GFF3884 0.30 -0.9 162,557 - GFF3884 0.30 +0.6 162,653 - GFF3884 0.38 +0.4 162,658 + GFF3884 0.38 -0.8 163,139 - GFF3884 0.78 -0.7 163,185 + GFF3884 0.81 -0.6 163,189 + GFF3884 0.82 +0.4
Or see this region's nucleotide sequence