Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3685 and GFF3686 are separated by 184 nucleotides GFF3686 and GFF3687 are separated by 289 nucleotides GFF3687 and GFF3688 are separated by 88 nucleotides GFF3688 and GFF3689 overlap by 44 nucleotides
GFF3685 - Aspartate aminotransferase (EC 2.6.1.1), at 40,006 to 41,196
GFF3685
GFF3686 - Outer membrane protein F precursor, at 41,381 to 42,472
GFF3686
GFF3687 - hypothetical protein, at 42,762 to 42,914
GFF3687
GFF3688 - Asparaginyl-tRNA synthetase (EC 6.1.1.22), at 43,003 to 43,122
GFF3688
GFF3689 - Asparaginyl-tRNA synthetase (EC 6.1.1.22), at 43,079 to 44,416
GFF3689
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 40.444 kb on - strand, within GFF3685 at 40.466 kb on + strand, within GFF3685 at 40.467 kb on - strand, within GFF3685 at 40.483 kb on - strand, within GFF3685 at 40.491 kb on + strand, within GFF3685 at 40.650 kb on + strand, within GFF3685 at 40.751 kb on + strand, within GFF3685 at 40.751 kb on + strand, within GFF3685 at 40.920 kb on + strand, within GFF3685 at 40.921 kb on - strand, within GFF3685 at 41.093 kb on - strand at 41.093 kb on - strand at 41.213 kb on - strand at 41.213 kb on - strand at 41.216 kb on + strand at 41.360 kb on + strand at 41.391 kb on + strand at 41.392 kb on - strand at 41.431 kb on - strand at 41.529 kb on + strand, within GFF3686 at 41.529 kb on + strand, within GFF3686 at 41.626 kb on - strand, within GFF3686 at 41.722 kb on - strand, within GFF3686 at 41.736 kb on + strand, within GFF3686 at 41.736 kb on + strand, within GFF3686 at 41.754 kb on + strand, within GFF3686 at 41.754 kb on + strand, within GFF3686 at 41.838 kb on + strand, within GFF3686 at 41.838 kb on + strand, within GFF3686 at 41.911 kb on + strand, within GFF3686 at 41.911 kb on + strand, within GFF3686 at 41.912 kb on - strand, within GFF3686 at 41.997 kb on + strand, within GFF3686 at 42.031 kb on - strand, within GFF3686 at 42.031 kb on - strand, within GFF3686 at 42.031 kb on - strand, within GFF3686 at 42.090 kb on + strand, within GFF3686 at 42.223 kb on + strand, within GFF3686 at 42.380 kb on - strand at 42.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 40,444 - GFF3685 0.37 +0.6 40,466 + GFF3685 0.39 +0.2 40,467 - GFF3685 0.39 +0.6 40,483 - GFF3685 0.40 -0.2 40,491 + GFF3685 0.41 -0.4 40,650 + GFF3685 0.54 -0.4 40,751 + GFF3685 0.63 +0.0 40,751 + GFF3685 0.63 +1.6 40,920 + GFF3685 0.77 -0.4 40,921 - GFF3685 0.77 +0.5 41,093 - -0.6 41,093 - +0.6 41,213 - -0.7 41,213 - -0.0 41,216 + +0.6 41,360 + -0.5 41,391 + +1.4 41,392 - +1.4 41,431 - +0.1 41,529 + GFF3686 0.14 -1.4 41,529 + GFF3686 0.14 -0.0 41,626 - GFF3686 0.22 -0.6 41,722 - GFF3686 0.31 +0.3 41,736 + GFF3686 0.33 -0.4 41,736 + GFF3686 0.33 -0.6 41,754 + GFF3686 0.34 +0.6 41,754 + GFF3686 0.34 +0.9 41,838 + GFF3686 0.42 -3.0 41,838 + GFF3686 0.42 +0.1 41,911 + GFF3686 0.49 -0.7 41,911 + GFF3686 0.49 -1.2 41,912 - GFF3686 0.49 +0.6 41,997 + GFF3686 0.56 +0.6 42,031 - GFF3686 0.60 -0.5 42,031 - GFF3686 0.60 -1.7 42,031 - GFF3686 0.60 +0.3 42,090 + GFF3686 0.65 -0.4 42,223 + GFF3686 0.77 -0.5 42,380 - -1.2 42,383 - +0.6
Or see this region's nucleotide sequence