Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3294 and GFF3295 are separated by 33 nucleotides GFF3295 and GFF3296 are separated by 20 nucleotides GFF3296 and GFF3297 are separated by 229 nucleotides GFF3297 and GFF3298 are separated by 39 nucleotides GFF3298 and GFF3299 are separated by 96 nucleotides
GFF3294 - Glucose-1-phosphatase (EC 3.1.3.10), at 65,566 to 66,807
GFF3294
GFF3295 - FIG00895798: hypothetical protein, at 66,841 to 67,068
GFF3295
GFF3296 - Flavoprotein WrbA, at 67,089 to 67,685
GFF3296
GFF3297 - FIG01045408: hypothetical protein, at 67,915 to 68,043
GFF3297
GFF3298 - FIG01049236: hypothetical protein, at 68,083 to 68,277
GFF3298
GFF3299 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 68,374 to 69,012
GFF3299
Position (kb) 67
68 Strain fitness (log2 ratio) -1
0
1
2 at 66.089 kb on + strand, within GFF3294 at 66.090 kb on - strand, within GFF3294 at 66.090 kb on - strand, within GFF3294 at 66.270 kb on + strand, within GFF3294 at 66.280 kb on + strand, within GFF3294 at 66.283 kb on + strand, within GFF3294 at 66.434 kb on - strand, within GFF3294 at 66.562 kb on + strand, within GFF3294 at 66.646 kb on + strand, within GFF3294 at 66.812 kb on - strand at 66.812 kb on - strand at 66.843 kb on - strand at 66.844 kb on + strand at 67.011 kb on + strand, within GFF3295 at 67.012 kb on - strand, within GFF3295 at 67.012 kb on - strand, within GFF3295 at 67.579 kb on - strand, within GFF3296 at 67.581 kb on - strand, within GFF3296 at 67.690 kb on - strand at 67.705 kb on - strand at 67.733 kb on + strand at 67.734 kb on - strand at 67.902 kb on + strand at 67.961 kb on + strand, within GFF3297 at 67.962 kb on - strand, within GFF3297 at 68.001 kb on + strand, within GFF3297 at 68.001 kb on + strand, within GFF3297 at 68.028 kb on + strand, within GFF3297 at 68.028 kb on + strand, within GFF3297 at 68.032 kb on + strand at 68.033 kb on - strand at 68.039 kb on + strand at 68.039 kb on + strand at 68.040 kb on - strand at 68.139 kb on - strand, within GFF3298 at 68.229 kb on - strand, within GFF3298 at 68.236 kb on - strand, within GFF3298 at 68.548 kb on + strand, within GFF3299 at 68.569 kb on + strand, within GFF3299 at 68.664 kb on + strand, within GFF3299
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 66,089 + GFF3294 0.42 +1.1 66,090 - GFF3294 0.42 +0.1 66,090 - GFF3294 0.42 +1.2 66,270 + GFF3294 0.57 -0.5 66,280 + GFF3294 0.57 -0.2 66,283 + GFF3294 0.58 +0.2 66,434 - GFF3294 0.70 +0.5 66,562 + GFF3294 0.80 +0.5 66,646 + GFF3294 0.87 -0.3 66,812 - +2.5 66,812 - +0.4 66,843 - +0.8 66,844 + +1.5 67,011 + GFF3295 0.75 +0.3 67,012 - GFF3295 0.75 +0.3 67,012 - GFF3295 0.75 -0.7 67,579 - GFF3296 0.82 +0.3 67,581 - GFF3296 0.82 -1.3 67,690 - +2.1 67,705 - -0.7 67,733 + -0.1 67,734 - -0.5 67,902 + +0.6 67,961 + GFF3297 0.36 +0.1 67,962 - GFF3297 0.36 +0.1 68,001 + GFF3297 0.67 -0.2 68,001 + GFF3297 0.67 +1.8 68,028 + GFF3297 0.88 -0.6 68,028 + GFF3297 0.88 -1.1 68,032 + +0.2 68,033 - -0.2 68,039 + +0.1 68,039 + +0.9 68,040 - +0.7 68,139 - GFF3298 0.29 +1.3 68,229 - GFF3298 0.75 +0.7 68,236 - GFF3298 0.78 -1.6 68,548 + GFF3299 0.27 +0.9 68,569 + GFF3299 0.31 +1.3 68,664 + GFF3299 0.45 +1.0
Or see this region's nucleotide sequence