Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3256 and GFF3257 are separated by 44 nucleotides GFF3257 and GFF3258 are separated by 57 nucleotides GFF3258 and GFF3259 are separated by 214 nucleotides GFF3259 and GFF3260 are separated by 16 nucleotides
GFF3256 - Succinyl-CoA synthetase, alpha subunit-related enzymes, at 31,776 to 32,192
GFF3256
GFF3257 - hemimethylated DNA binding protein YccV, at 32,237 to 32,554
GFF3257
GFF3258 - LSU m5C1962 methyltransferase RlmI, at 32,612 to 33,823
GFF3258
GFF3259 - Protein ybcL precursor, at 34,038 to 34,586
GFF3259
GFF3260 - AraC family transcription regulator, at 34,603 to 35,391
GFF3260
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1
2
3 at 31.658 kb on + strand at 31.677 kb on + strand at 31.677 kb on + strand at 31.679 kb on + strand at 31.679 kb on + strand at 31.685 kb on + strand at 31.685 kb on + strand at 31.686 kb on - strand at 31.706 kb on + strand at 31.706 kb on + strand at 31.732 kb on + strand at 31.823 kb on + strand, within GFF3256 at 31.835 kb on - strand, within GFF3256 at 32.049 kb on - strand, within GFF3256 at 32.356 kb on - strand, within GFF3257 at 32.356 kb on - strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.499 kb on + strand, within GFF3257 at 32.573 kb on - strand at 32.573 kb on - strand at 32.585 kb on + strand at 32.613 kb on + strand at 32.705 kb on - strand at 32.729 kb on + strand at 33.193 kb on - strand, within GFF3258 at 33.193 kb on - strand, within GFF3258 at 33.498 kb on + strand, within GFF3258 at 33.502 kb on + strand, within GFF3258 at 33.812 kb on - strand at 33.865 kb on - strand at 33.907 kb on - strand at 33.963 kb on + strand at 33.972 kb on - strand at 34.018 kb on + strand at 34.018 kb on + strand at 34.184 kb on + strand, within GFF3259 at 34.245 kb on + strand, within GFF3259 at 34.250 kb on + strand, within GFF3259 at 34.254 kb on - strand, within GFF3259 at 34.254 kb on - strand, within GFF3259 at 34.530 kb on - strand, within GFF3259 at 34.530 kb on - strand, within GFF3259 at 34.575 kb on + strand at 34.576 kb on - strand at 34.713 kb on + strand, within GFF3260 at 34.714 kb on - strand, within GFF3260 at 34.725 kb on + strand, within GFF3260
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 31,658 + -0.9 31,677 + -0.2 31,677 + -0.6 31,679 + -0.2 31,679 + -0.4 31,685 + +0.9 31,685 + +1.4 31,686 - +0.8 31,706 + -0.0 31,706 + -0.9 31,732 + +0.0 31,823 + GFF3256 0.11 -0.7 31,835 - GFF3256 0.14 -0.3 32,049 - GFF3256 0.65 +2.2 32,356 - GFF3257 0.37 -0.1 32,356 - GFF3257 0.37 +0.0 32,359 + GFF3257 0.38 +0.4 32,359 + GFF3257 0.38 -0.6 32,359 + GFF3257 0.38 +0.1 32,359 + GFF3257 0.38 -0.6 32,499 + GFF3257 0.82 +0.2 32,573 - +0.3 32,573 - -0.6 32,585 + +0.6 32,613 + -1.1 32,705 - -1.4 32,729 + -1.2 33,193 - GFF3258 0.48 -0.8 33,193 - GFF3258 0.48 -0.3 33,498 + GFF3258 0.73 -0.2 33,502 + GFF3258 0.73 -1.0 33,812 - +3.7 33,865 - -0.4 33,907 - +1.2 33,963 + -0.1 33,972 - -1.1 34,018 + +0.2 34,018 + -0.2 34,184 + GFF3259 0.27 -0.0 34,245 + GFF3259 0.38 -0.7 34,250 + GFF3259 0.39 -0.4 34,254 - GFF3259 0.39 +0.5 34,254 - GFF3259 0.39 +0.8 34,530 - GFF3259 0.90 -0.5 34,530 - GFF3259 0.90 +2.5 34,575 + -0.6 34,576 - -0.4 34,713 + GFF3260 0.14 -0.2 34,714 - GFF3260 0.14 -1.3 34,725 + GFF3260 0.15 -1.0
Or see this region's nucleotide sequence