Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF302 and GFF303 are separated by 11 nucleotides GFF303 and GFF304 are separated by 74 nucleotides GFF304 and GFF305 overlap by 1 nucleotides
GFF302 - Homolog of fucose/glucose/galactose permeases, at 19,518 to 20,834
GFF302
GFF303 - Putative uncharacterized protein STY3991, at 20,846 to 21,859
GFF303
GFF304 - Transcriptional regulatory protein UhpA, at 21,934 to 22,524
GFF304
GFF305 - Sensor histidine protein kinase UhpB, glucose-6-phosphate specific (EC 2.7.13.3), at 22,524 to 24,026
GFF305
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 19.849 kb on - strand, within GFF302 at 19.849 kb on - strand, within GFF302 at 19.921 kb on - strand, within GFF302 at 20.022 kb on + strand, within GFF302 at 20.022 kb on + strand, within GFF302 at 20.132 kb on + strand, within GFF302 at 20.132 kb on + strand, within GFF302 at 20.133 kb on - strand, within GFF302 at 20.136 kb on + strand, within GFF302 at 20.136 kb on + strand, within GFF302 at 20.137 kb on - strand, within GFF302 at 20.144 kb on + strand, within GFF302 at 20.167 kb on + strand, within GFF302 at 20.243 kb on + strand, within GFF302 at 20.254 kb on - strand, within GFF302 at 20.305 kb on - strand, within GFF302 at 20.400 kb on + strand, within GFF302 at 20.401 kb on - strand, within GFF302 at 20.515 kb on - strand, within GFF302 at 20.548 kb on - strand, within GFF302 at 20.616 kb on - strand, within GFF302 at 20.703 kb on + strand at 20.860 kb on + strand at 20.896 kb on + strand at 20.897 kb on - strand at 20.921 kb on - strand at 21.104 kb on + strand, within GFF303 at 21.104 kb on + strand, within GFF303 at 21.105 kb on - strand, within GFF303 at 21.163 kb on + strand, within GFF303 at 21.163 kb on + strand, within GFF303 at 21.261 kb on - strand, within GFF303 at 21.261 kb on - strand, within GFF303 at 21.314 kb on - strand, within GFF303 at 21.383 kb on + strand, within GFF303 at 21.383 kb on + strand, within GFF303 at 21.384 kb on - strand, within GFF303 at 21.384 kb on - strand, within GFF303 at 21.401 kb on + strand, within GFF303 at 21.401 kb on + strand, within GFF303 at 21.401 kb on + strand, within GFF303 at 21.402 kb on - strand, within GFF303 at 21.402 kb on - strand, within GFF303 at 21.407 kb on + strand, within GFF303 at 21.407 kb on + strand, within GFF303 at 21.611 kb on + strand, within GFF303 at 21.714 kb on - strand, within GFF303 at 21.714 kb on - strand, within GFF303 at 21.899 kb on + strand at 22.077 kb on - strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.082 kb on + strand, within GFF304 at 22.083 kb on - strand, within GFF304 at 22.096 kb on - strand, within GFF304 at 22.110 kb on + strand, within GFF304 at 22.233 kb on + strand, within GFF304 at 22.273 kb on - strand, within GFF304 at 22.273 kb on - strand, within GFF304 at 22.294 kb on + strand, within GFF304 at 22.295 kb on - strand, within GFF304 at 22.397 kb on + strand, within GFF304 at 22.522 kb on + strand at 22.523 kb on - strand at 22.676 kb on + strand, within GFF305 at 22.677 kb on - strand, within GFF305 at 22.737 kb on + strand, within GFF305 at 22.858 kb on + strand, within GFF305
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 19,849 - GFF302 0.25 -0.9 19,849 - GFF302 0.25 -0.2 19,921 - GFF302 0.31 -1.4 20,022 + GFF302 0.38 -0.3 20,022 + GFF302 0.38 +0.6 20,132 + GFF302 0.47 -0.1 20,132 + GFF302 0.47 +0.2 20,133 - GFF302 0.47 -0.8 20,136 + GFF302 0.47 -0.0 20,136 + GFF302 0.47 -0.2 20,137 - GFF302 0.47 -0.1 20,144 + GFF302 0.48 -0.1 20,167 + GFF302 0.49 -0.4 20,243 + GFF302 0.55 +1.4 20,254 - GFF302 0.56 -0.3 20,305 - GFF302 0.60 -0.7 20,400 + GFF302 0.67 +0.2 20,401 - GFF302 0.67 -1.2 20,515 - GFF302 0.76 -0.4 20,548 - GFF302 0.78 -1.7 20,616 - GFF302 0.83 +0.9 20,703 + +0.4 20,860 + +1.4 20,896 + +0.7 20,897 - -0.6 20,921 - +0.4 21,104 + GFF303 0.25 +0.3 21,104 + GFF303 0.25 +0.3 21,105 - GFF303 0.26 -0.7 21,163 + GFF303 0.31 -0.1 21,163 + GFF303 0.31 +0.6 21,261 - GFF303 0.41 -0.2 21,261 - GFF303 0.41 -0.1 21,314 - GFF303 0.46 +0.2 21,383 + GFF303 0.53 -0.3 21,383 + GFF303 0.53 +0.4 21,384 - GFF303 0.53 +0.6 21,384 - GFF303 0.53 +0.7 21,401 + GFF303 0.55 +0.4 21,401 + GFF303 0.55 -0.9 21,401 + GFF303 0.55 +1.3 21,402 - GFF303 0.55 -0.0 21,402 - GFF303 0.55 -0.7 21,407 + GFF303 0.55 +0.1 21,407 + GFF303 0.55 -2.2 21,611 + GFF303 0.75 +0.0 21,714 - GFF303 0.86 +0.2 21,714 - GFF303 0.86 +1.0 21,899 + +0.4 22,077 - GFF304 0.24 -0.5 22,082 + GFF304 0.25 -0.1 22,082 + GFF304 0.25 +0.9 22,082 + GFF304 0.25 -0.1 22,082 + GFF304 0.25 +0.8 22,083 - GFF304 0.25 +0.3 22,096 - GFF304 0.27 +1.1 22,110 + GFF304 0.30 +0.6 22,233 + GFF304 0.51 +0.6 22,273 - GFF304 0.57 +0.3 22,273 - GFF304 0.57 -0.1 22,294 + GFF304 0.61 +0.8 22,295 - GFF304 0.61 +0.1 22,397 + GFF304 0.78 +0.7 22,522 + +0.6 22,523 - +1.8 22,676 + GFF305 0.10 +0.4 22,677 - GFF305 0.10 +0.8 22,737 + GFF305 0.14 -0.0 22,858 + GFF305 0.22 +0.4
Or see this region's nucleotide sequence