Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2829 and GFF2830 are separated by 21 nucleotides GFF2830 and GFF2831 are separated by 472 nucleotides
GFF2829 - Rtn protein, at 350,150 to 351,751
GFF2829
GFF2830 - Magnesium and cobalt efflux protein CorC, at 351,773 to 353,332
GFF2830
GFF2831 - PTS system, mannose-specific IIA component (EC 2.7.1.69) / PTS system, mannose-specific IIB component (EC 2.7.1.69), at 353,805 to 354,773
GFF2831
Position (kb)
351
352
353
354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 350.776 kb on - strand, within GFF2829 at 350.824 kb on + strand, within GFF2829 at 350.824 kb on + strand, within GFF2829 at 350.856 kb on + strand, within GFF2829 at 350.923 kb on + strand, within GFF2829 at 350.924 kb on - strand, within GFF2829 at 351.529 kb on + strand, within GFF2829 at 351.639 kb on - strand at 351.644 kb on + strand at 351.747 kb on + strand at 351.748 kb on - strand at 351.898 kb on + strand at 352.215 kb on - strand, within GFF2830 at 352.544 kb on - strand, within GFF2830 at 352.628 kb on - strand, within GFF2830 at 352.826 kb on + strand, within GFF2830 at 352.881 kb on - strand, within GFF2830 at 352.885 kb on + strand, within GFF2830 at 352.950 kb on - strand, within GFF2830 at 353.049 kb on - strand, within GFF2830 at 353.050 kb on + strand, within GFF2830 at 353.090 kb on - strand, within GFF2830 at 353.412 kb on + strand at 353.412 kb on + strand at 353.412 kb on + strand at 353.412 kb on + strand at 353.429 kb on - strand at 353.488 kb on - strand at 353.759 kb on + strand at 353.816 kb on + strand at 353.825 kb on - strand at 353.825 kb on - strand at 353.966 kb on + strand, within GFF2831 at 353.981 kb on + strand, within GFF2831 at 354.005 kb on + strand, within GFF2831 at 354.006 kb on - strand, within GFF2831
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 350,776 - GFF2829 0.39 +0.1 350,824 + GFF2829 0.42 +1.5 350,824 + GFF2829 0.42 +0.1 350,856 + GFF2829 0.44 +0.3 350,923 + GFF2829 0.48 -0.5 350,924 - GFF2829 0.48 -0.1 351,529 + GFF2829 0.86 +0.1 351,639 - +0.1 351,644 + +0.3 351,747 + +0.6 351,748 - +0.6 351,898 + -0.0 352,215 - GFF2830 0.28 -0.4 352,544 - GFF2830 0.49 +1.8 352,628 - GFF2830 0.55 -0.0 352,826 + GFF2830 0.68 +0.0 352,881 - GFF2830 0.71 -0.3 352,885 + GFF2830 0.71 -0.0 352,950 - GFF2830 0.75 -0.8 353,049 - GFF2830 0.82 -0.6 353,050 + GFF2830 0.82 -0.6 353,090 - GFF2830 0.84 +1.1 353,412 + -0.2 353,412 + +0.8 353,412 + -2.7 353,412 + +1.0 353,429 - +0.0 353,488 - -0.6 353,759 + -0.6 353,816 + -0.4 353,825 - +0.8 353,825 - -0.6 353,966 + GFF2831 0.17 -3.0 353,981 + GFF2831 0.18 +0.7 354,005 + GFF2831 0.21 +0.5 354,006 - GFF2831 0.21 +0.5
Or see this region's nucleotide sequence