Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2750

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2749 and GFF2750 are separated by 196 nucleotidesGFF2750 and GFF2751 are separated by 662 nucleotides GFF2749 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 268,746 to 271,424 GFF2749 GFF2750 - Thymidine kinase (EC 2.7.1.21), at 271,621 to 272,238 GFF2750 GFF2751 - DNA-binding protein H-NS, at 272,901 to 273,314 GFF2751 Position (kb) 271 272 273Strain fitness (log2 ratio) -3 -2 -1 0 1at 270.907 kb on + strand, within GFF2749at 270.908 kb on - strand, within GFF2749at 270.983 kb on + strand, within GFF2749at 270.997 kb on - strand, within GFF2749at 271.042 kb on - strand, within GFF2749at 271.329 kb on + strandat 271.510 kb on + strandat 271.567 kb on - strandat 271.620 kb on - strandat 271.620 kb on - strandat 271.624 kb on + strandat 271.625 kb on - strandat 271.625 kb on - strandat 271.681 kb on - strandat 271.783 kb on - strand, within GFF2750at 271.915 kb on - strand, within GFF2750at 271.918 kb on + strand, within GFF2750at 271.919 kb on - strand, within GFF2750at 271.996 kb on + strand, within GFF2750at 271.996 kb on + strand, within GFF2750at 272.044 kb on - strand, within GFF2750at 272.131 kb on - strand, within GFF2750at 272.135 kb on - strand, within GFF2750at 272.135 kb on - strand, within GFF2750at 272.225 kb on + strandat 272.226 kb on - strandat 272.289 kb on + strandat 272.482 kb on + strandat 272.638 kb on + strandat 272.816 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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270,907 + GFF2749 0.81 -1.2
270,908 - GFF2749 0.81 +1.3
270,983 + GFF2749 0.84 +0.8
270,997 - GFF2749 0.84 -0.3
271,042 - GFF2749 0.86 +0.1
271,329 + +0.6
271,510 + -0.5
271,567 - -1.5
271,620 - -0.3
271,620 - -0.4
271,624 + -2.2
271,625 - -1.1
271,625 - -0.5
271,681 - +0.4
271,783 - GFF2750 0.26 -2.9
271,915 - GFF2750 0.48 +0.1
271,918 + GFF2750 0.48 -1.1
271,919 - GFF2750 0.48 -2.2
271,996 + GFF2750 0.61 +0.9
271,996 + GFF2750 0.61 +0.3
272,044 - GFF2750 0.68 +0.5
272,131 - GFF2750 0.83 -1.5
272,135 - GFF2750 0.83 +0.6
272,135 - GFF2750 0.83 +1.3
272,225 + -0.9
272,226 - -0.3
272,289 + +0.8
272,482 + +0.1
272,638 + -0.0
272,816 + -1.1

Or see this region's nucleotide sequence