Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1944 and GFF1945 are separated by 159 nucleotides GFF1945 and GFF1946 are separated by 51 nucleotides GFF1946 and GFF1947 are separated by 32 nucleotides GFF1947 and GFF1948 overlap by 1 nucleotides
GFF1944 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASI (EC 2.3.1.41), at 135,012 to 136,226
GFF1944
GFF1945 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 136,386 to 138,386
GFF1945
GFF1946 - FIG00639460: hypothetical protein, at 138,438 to 138,713
GFF1946
GFF1947 - Putative transporting ATPase, at 138,746 to 139,294
GFF1947
GFF1948 - Putative membrane protein YfcA, at 139,294 to 140,103
GFF1948
Position (kb) 136
137
138
139 Strain fitness (log2 ratio) -1
0
1 at 136.378 kb on + strand at 136.384 kb on - strand at 136.402 kb on + strand at 136.561 kb on + strand at 136.562 kb on - strand at 137.394 kb on + strand, within GFF1945 at 137.395 kb on - strand, within GFF1945 at 137.395 kb on - strand, within GFF1945 at 137.723 kb on + strand, within GFF1945 at 137.840 kb on + strand, within GFF1945 at 137.881 kb on + strand, within GFF1945 at 137.896 kb on - strand, within GFF1945 at 137.918 kb on + strand, within GFF1945 at 137.919 kb on - strand, within GFF1945 at 137.934 kb on - strand, within GFF1945 at 138.133 kb on - strand, within GFF1945 at 138.174 kb on - strand, within GFF1945 at 138.202 kb on - strand at 138.216 kb on - strand at 138.291 kb on - strand at 138.362 kb on - strand at 138.553 kb on + strand, within GFF1946 at 138.554 kb on - strand, within GFF1946 at 138.729 kb on + strand at 138.730 kb on - strand at 138.804 kb on + strand, within GFF1947 at 138.869 kb on + strand, within GFF1947 at 139.228 kb on + strand, within GFF1947 at 139.340 kb on + strand at 139.340 kb on + strand at 139.341 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 136,378 + +0.8 136,384 - -0.1 136,402 + -0.3 136,561 + +0.6 136,562 - -0.3 137,394 + GFF1945 0.50 +0.8 137,395 - GFF1945 0.50 -0.3 137,395 - GFF1945 0.50 +0.2 137,723 + GFF1945 0.67 +0.3 137,840 + GFF1945 0.73 +0.6 137,881 + GFF1945 0.75 -0.2 137,896 - GFF1945 0.75 +0.8 137,918 + GFF1945 0.77 -0.8 137,919 - GFF1945 0.77 +0.0 137,934 - GFF1945 0.77 +1.6 138,133 - GFF1945 0.87 +1.0 138,174 - GFF1945 0.89 +1.1 138,202 - -0.5 138,216 - +0.4 138,291 - -0.0 138,362 - +0.2 138,553 + GFF1946 0.42 -0.6 138,554 - GFF1946 0.42 +0.4 138,729 + +1.4 138,730 - -1.1 138,804 + GFF1947 0.11 +0.6 138,869 + GFF1947 0.22 -0.7 139,228 + GFF1947 0.88 -0.4 139,340 + +0.7 139,340 + +1.0 139,341 - -0.1
Or see this region's nucleotide sequence