Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1919 and GFF1920 are separated by 77 nucleotides GFF1920 and GFF1921 are separated by 88 nucleotides GFF1921 and GFF1922 are separated by 26 nucleotides GFF1922 and GFF1923 are separated by 63 nucleotides
GFF1919 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 111,901 to 112,683
GFF1919
GFF1920 - hypothetical protein, at 112,761 to 112,883
GFF1920
GFF1921 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 112,972 to 113,541
GFF1921
GFF1922 - Lysine-specific permease, at 113,568 to 114,974
GFF1922
GFF1923 - FIG074102: hypothetical protein, at 115,038 to 116,141
GFF1923
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1 at 112.577 kb on + strand, within GFF1919 at 112.584 kb on + strand, within GFF1919 at 112.584 kb on + strand, within GFF1919 at 112.584 kb on + strand, within GFF1919 at 112.585 kb on - strand, within GFF1919 at 112.585 kb on - strand, within GFF1919 at 112.841 kb on - strand, within GFF1920 at 112.949 kb on + strand at 113.119 kb on + strand, within GFF1921 at 113.244 kb on + strand, within GFF1921 at 113.245 kb on - strand, within GFF1921 at 113.482 kb on + strand, within GFF1921 at 113.496 kb on - strand at 113.524 kb on + strand at 113.640 kb on - strand at 113.661 kb on + strand at 113.668 kb on - strand at 113.718 kb on - strand, within GFF1922 at 113.878 kb on - strand, within GFF1922 at 113.881 kb on + strand, within GFF1922 at 113.900 kb on + strand, within GFF1922 at 113.948 kb on + strand, within GFF1922 at 113.981 kb on + strand, within GFF1922 at 114.341 kb on - strand, within GFF1922 at 114.493 kb on + strand, within GFF1922 at 114.567 kb on + strand, within GFF1922 at 114.569 kb on + strand, within GFF1922 at 114.569 kb on + strand, within GFF1922 at 114.569 kb on + strand, within GFF1922 at 114.570 kb on - strand, within GFF1922 at 114.570 kb on - strand, within GFF1922 at 114.570 kb on - strand, within GFF1922 at 114.571 kb on + strand, within GFF1922 at 114.572 kb on - strand, within GFF1922 at 114.606 kb on - strand, within GFF1922 at 114.606 kb on - strand, within GFF1922 at 114.677 kb on - strand, within GFF1922 at 114.677 kb on - strand, within GFF1922 at 114.783 kb on - strand, within GFF1922 at 114.803 kb on - strand, within GFF1922 at 114.803 kb on - strand, within GFF1922 at 114.810 kb on + strand, within GFF1922 at 114.858 kb on - strand at 114.861 kb on + strand at 115.019 kb on + strand at 115.095 kb on - strand at 115.097 kb on - strand at 115.167 kb on - strand, within GFF1923 at 115.191 kb on - strand, within GFF1923 at 115.293 kb on - strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.378 kb on + strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.379 kb on - strand, within GFF1923 at 115.382 kb on + strand, within GFF1923 at 115.531 kb on + strand, within GFF1923 at 115.700 kb on + strand, within GFF1923 at 115.749 kb on - strand, within GFF1923 at 115.859 kb on + strand, within GFF1923 at 115.912 kb on - strand, within GFF1923 at 115.925 kb on + strand, within GFF1923 at 115.926 kb on - strand, within GFF1923
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 112,577 + GFF1919 0.86 -0.8 112,584 + GFF1919 0.87 +0.3 112,584 + GFF1919 0.87 -0.3 112,584 + GFF1919 0.87 -0.3 112,585 - GFF1919 0.87 +1.2 112,585 - GFF1919 0.87 -0.7 112,841 - GFF1920 0.65 +0.1 112,949 + +0.9 113,119 + GFF1921 0.26 -0.6 113,244 + GFF1921 0.48 -0.9 113,245 - GFF1921 0.48 -0.3 113,482 + GFF1921 0.89 -0.9 113,496 - -1.8 113,524 + +0.4 113,640 - +0.4 113,661 + +1.7 113,668 - -0.9 113,718 - GFF1922 0.11 -0.6 113,878 - GFF1922 0.22 -2.0 113,881 + GFF1922 0.22 -1.6 113,900 + GFF1922 0.24 +0.3 113,948 + GFF1922 0.27 +0.1 113,981 + GFF1922 0.29 +0.2 114,341 - GFF1922 0.55 +0.4 114,493 + GFF1922 0.66 -0.6 114,567 + GFF1922 0.71 -1.3 114,569 + GFF1922 0.71 -0.3 114,569 + GFF1922 0.71 -0.6 114,569 + GFF1922 0.71 -0.4 114,570 - GFF1922 0.71 +1.7 114,570 - GFF1922 0.71 -1.6 114,570 - GFF1922 0.71 -0.2 114,571 + GFF1922 0.71 -0.2 114,572 - GFF1922 0.71 +0.4 114,606 - GFF1922 0.74 -0.9 114,606 - GFF1922 0.74 +0.6 114,677 - GFF1922 0.79 +1.1 114,677 - GFF1922 0.79 +0.9 114,783 - GFF1922 0.86 +0.0 114,803 - GFF1922 0.88 -0.6 114,803 - GFF1922 0.88 -0.3 114,810 + GFF1922 0.88 -0.4 114,858 - -0.6 114,861 + -0.5 115,019 + +0.1 115,095 - -1.2 115,097 - +0.2 115,167 - GFF1923 0.12 -1.1 115,191 - GFF1923 0.14 -0.1 115,293 - GFF1923 0.23 -1.4 115,378 + GFF1923 0.31 -1.2 115,378 + GFF1923 0.31 +0.9 115,378 + GFF1923 0.31 -0.1 115,378 + GFF1923 0.31 -0.6 115,379 - GFF1923 0.31 -0.5 115,379 - GFF1923 0.31 -0.4 115,379 - GFF1923 0.31 -0.3 115,379 - GFF1923 0.31 -0.5 115,382 + GFF1923 0.31 +0.3 115,531 + GFF1923 0.45 -1.8 115,700 + GFF1923 0.60 -0.4 115,749 - GFF1923 0.64 +0.1 115,859 + GFF1923 0.74 +0.3 115,912 - GFF1923 0.79 -0.4 115,925 + GFF1923 0.80 -0.4 115,926 - GFF1923 0.80 -0.5
Or see this region's nucleotide sequence