Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1642

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1639 and GFF1640 are separated by 8 nucleotidesGFF1640 and GFF1641 are separated by 174 nucleotidesGFF1641 and GFF1642 are separated by 32 nucleotidesGFF1642 and GFF1643 are separated by 626 nucleotides GFF1639 - Holliday junction DNA helicase RuvB, at 25,475 to 26,485 GFF1639 GFF1640 - Holliday junction DNA helicase RuvA, at 26,494 to 27,105 GFF1640 GFF1641 - hypothetical protein, at 27,280 to 27,405 GFF1641 GFF1642 - putative cytoplasmic protein, at 27,438 to 28,421 GFF1642 GFF1643 - hypothetical protein, at 29,048 to 29,647 GFF1643 Position (kb) 27 28 29Strain fitness (log2 ratio) -1 0 1at 27.019 kb on - strand, within GFF1640at 27.150 kb on - strandat 27.196 kb on + strandat 27.390 kb on - strand, within GFF1641at 27.553 kb on - strand, within GFF1642at 29.168 kb on - strand, within GFF1643at 29.170 kb on + strand, within GFF1643at 29.171 kb on - strand, within GFF1643at 29.192 kb on + strand, within GFF1643at 29.249 kb on - strand, within GFF1643at 29.378 kb on + strand, within GFF1643at 29.420 kb on - strand, within GFF1643

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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27,019 - GFF1640 0.86 -1.5
27,150 - +0.1
27,196 + +0.5
27,390 - GFF1641 0.87 -0.6
27,553 - GFF1642 0.12 +0.2
29,168 - GFF1643 0.20 -0.4
29,170 + GFF1643 0.20 +0.5
29,171 - GFF1643 0.20 +0.1
29,192 + GFF1643 0.24 -0.0
29,249 - GFF1643 0.34 +0.5
29,378 + GFF1643 0.55 +1.0
29,420 - GFF1643 0.62 -0.0

Or see this region's nucleotide sequence