Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1400 and GFF1401 are separated by 142 nucleotides GFF1401 and GFF1402 are separated by 165 nucleotides GFF1402 and GFF1403 are separated by 14 nucleotides
GFF1400 - YjbH outer membrane lipoprotein, at 44,063 to 46,159
GFF1400
GFF1401 - PsiE protein, at 46,302 to 46,712
GFF1401
GFF1402 - Maltose/maltodextrin ABC transporter, permease protein MalG, at 46,878 to 47,768
GFF1402
GFF1403 - Maltose/maltodextrin ABC transporter, permease protein MalF, at 47,783 to 49,327
GFF1403
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 45.952 kb on + strand at 46.002 kb on + strand at 46.127 kb on - strand at 46.127 kb on - strand at 46.208 kb on - strand at 46.240 kb on + strand at 46.264 kb on + strand at 46.465 kb on - strand, within GFF1401 at 46.492 kb on - strand, within GFF1401 at 46.535 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.634 kb on - strand, within GFF1401 at 46.634 kb on - strand, within GFF1401 at 46.636 kb on + strand, within GFF1401 at 46.636 kb on + strand, within GFF1401 at 46.637 kb on - strand, within GFF1401 at 46.668 kb on - strand, within GFF1401 at 46.685 kb on + strand at 46.685 kb on + strand at 46.710 kb on + strand at 46.737 kb on - strand at 46.767 kb on + strand at 46.811 kb on + strand at 46.811 kb on + strand at 46.827 kb on - strand at 46.872 kb on + strand at 47.040 kb on - strand, within GFF1402 at 47.040 kb on - strand, within GFF1402 at 47.041 kb on + strand, within GFF1402 at 47.041 kb on + strand, within GFF1402 at 47.111 kb on + strand, within GFF1402 at 47.111 kb on + strand, within GFF1402 at 47.230 kb on + strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.489 kb on + strand, within GFF1402 at 47.653 kb on - strand, within GFF1402 at 47.772 kb on + strand at 47.772 kb on + strand at 47.772 kb on + strand at 47.773 kb on - strand at 47.775 kb on + strand at 47.775 kb on + strand at 47.775 kb on + strand at 47.914 kb on - strand at 47.914 kb on - strand at 47.920 kb on - strand at 48.180 kb on + strand, within GFF1403 at 48.181 kb on - strand, within GFF1403 at 48.379 kb on - strand, within GFF1403 at 48.383 kb on - strand, within GFF1403 at 48.411 kb on - strand, within GFF1403 at 48.557 kb on - strand, within GFF1403 at 48.562 kb on + strand, within GFF1403 at 48.562 kb on + strand, within GFF1403 at 48.563 kb on - strand, within GFF1403 at 48.563 kb on - strand, within GFF1403 at 48.563 kb on - strand, within GFF1403 at 48.564 kb on + strand, within GFF1403 at 48.565 kb on - strand, within GFF1403 at 48.646 kb on - strand, within GFF1403 at 48.746 kb on - strand, within GFF1403 at 48.746 kb on - strand, within GFF1403
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 45,952 + +0.5 46,002 + -0.3 46,127 - +0.0 46,127 - +0.0 46,208 - +0.0 46,240 + -0.9 46,264 + +0.8 46,465 - GFF1401 0.40 -1.2 46,492 - GFF1401 0.46 -0.0 46,535 + GFF1401 0.57 +0.7 46,633 + GFF1401 0.81 -0.5 46,633 + GFF1401 0.81 -0.5 46,633 + GFF1401 0.81 +0.0 46,633 + GFF1401 0.81 -2.1 46,634 - GFF1401 0.81 -0.0 46,634 - GFF1401 0.81 -0.6 46,636 + GFF1401 0.81 -0.1 46,636 + GFF1401 0.81 +0.4 46,637 - GFF1401 0.82 +0.2 46,668 - GFF1401 0.89 +0.1 46,685 + +0.7 46,685 + -0.3 46,710 + +0.1 46,737 - +0.1 46,767 + -0.3 46,811 + -0.2 46,811 + +0.2 46,827 - +0.1 46,872 + -0.2 47,040 - GFF1402 0.18 -0.1 47,040 - GFF1402 0.18 -0.8 47,041 + GFF1402 0.18 -0.1 47,041 + GFF1402 0.18 +2.9 47,111 + GFF1402 0.26 +0.3 47,111 + GFF1402 0.26 +0.4 47,230 + GFF1402 0.40 +0.2 47,321 - GFF1402 0.50 +0.5 47,321 - GFF1402 0.50 +0.7 47,321 - GFF1402 0.50 +0.2 47,321 - GFF1402 0.50 +0.3 47,321 - GFF1402 0.50 +0.1 47,489 + GFF1402 0.69 +0.4 47,653 - GFF1402 0.87 -0.4 47,772 + +0.1 47,772 + +0.2 47,772 + -1.1 47,773 - -0.5 47,775 + -0.1 47,775 + -0.9 47,775 + +0.4 47,914 - -0.2 47,914 - +1.1 47,920 - +0.5 48,180 + GFF1403 0.26 -1.3 48,181 - GFF1403 0.26 -0.4 48,379 - GFF1403 0.39 -2.9 48,383 - GFF1403 0.39 -1.6 48,411 - GFF1403 0.41 +0.1 48,557 - GFF1403 0.50 -0.1 48,562 + GFF1403 0.50 -0.0 48,562 + GFF1403 0.50 -0.1 48,563 - GFF1403 0.50 -1.2 48,563 - GFF1403 0.50 -1.0 48,563 - GFF1403 0.50 +0.4 48,564 + GFF1403 0.51 -1.1 48,565 - GFF1403 0.51 -2.0 48,646 - GFF1403 0.56 +0.1 48,746 - GFF1403 0.62 -0.4 48,746 - GFF1403 0.62 +0.3
Or see this region's nucleotide sequence