Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1357

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1355 and GFF1356 overlap by 17 nucleotidesGFF1356 and GFF1357 are separated by 123 nucleotidesGFF1357 and GFF1358 are separated by 48 nucleotides GFF1355 - hypothetical protein, at 6,921 to 7,355 GFF1355 GFF1356 - Acetate operon repressor, at 7,339 to 8,163 GFF1356 GFF1357 - hypothetical protein, at 8,287 to 8,418 GFF1357 GFF1358 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), at 8,467 to 12,150 GFF1358 Position (kb) 8 9Strain fitness (log2 ratio) -1 0 1at 7.355 kb on + strandat 7.355 kb on + strandat 7.485 kb on + strand, within GFF1356at 7.605 kb on + strand, within GFF1356at 8.214 kb on - strandat 8.310 kb on + strand, within GFF1357at 8.541 kb on + strandat 8.541 kb on + strandat 8.554 kb on + strandat 8.561 kb on + strandat 8.677 kb on + strandat 8.719 kb on - strandat 8.780 kb on - strandat 8.922 kb on + strand, within GFF1358at 8.926 kb on - strand, within GFF1358at 9.160 kb on + strand, within GFF1358at 9.161 kb on - strand, within GFF1358at 9.163 kb on - strand, within GFF1358at 9.201 kb on + strand, within GFF1358at 9.236 kb on - strand, within GFF1358at 9.317 kb on - strand, within GFF1358at 9.417 kb on + strand, within GFF1358

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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7,355 + +0.2
7,355 + +0.4
7,485 + GFF1356 0.18 -0.2
7,605 + GFF1356 0.32 +0.8
8,214 - +1.4
8,310 + GFF1357 0.17 +0.4
8,541 + -0.0
8,541 + +0.3
8,554 + +1.1
8,561 + +0.1
8,677 + -0.2
8,719 - -0.0
8,780 - +1.0
8,922 + GFF1358 0.12 +0.3
8,926 - GFF1358 0.12 +0.8
9,160 + GFF1358 0.19 +0.6
9,161 - GFF1358 0.19 +1.1
9,163 - GFF1358 0.19 +1.3
9,201 + GFF1358 0.20 +1.2
9,236 - GFF1358 0.21 +0.9
9,317 - GFF1358 0.23 +1.0
9,417 + GFF1358 0.26 -1.6

Or see this region's nucleotide sequence