Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1266 and GFF1267 overlap by 20 nucleotides GFF1267 and GFF1268 are separated by 290 nucleotides GFF1268 and GFF1269 are separated by 66 nucleotides GFF1269 and GFF1270 are separated by 235 nucleotides
GFF1266 - FIG00638373: hypothetical protein, at 91,145 to 91,909
GFF1266
GFF1267 - FIG01047435: hypothetical protein, at 91,890 to 92,114
GFF1267
GFF1268 - Putative antirestriction protein, at 92,405 to 92,839
GFF1268
GFF1269 - FIG074102: hypothetical protein, at 92,906 to 93,340
GFF1269
GFF1270 - DNA polymerase III theta subunit (EC 2.7.7.7), at 93,576 to 93,899
GFF1270
Position (kb) 92
93 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 91.562 kb on - strand, within GFF1266 at 91.652 kb on + strand, within GFF1266 at 91.652 kb on + strand, within GFF1266 at 91.663 kb on + strand, within GFF1266 at 91.663 kb on + strand, within GFF1266 at 91.722 kb on + strand, within GFF1266 at 91.911 kb on + strand at 91.912 kb on - strand at 91.933 kb on - strand, within GFF1267 at 92.161 kb on - strand at 92.386 kb on - strand at 92.404 kb on + strand at 92.405 kb on - strand at 92.405 kb on - strand at 92.405 kb on - strand at 92.437 kb on + strand at 92.440 kb on + strand at 92.512 kb on - strand, within GFF1268 at 92.512 kb on - strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.520 kb on - strand, within GFF1268 at 92.520 kb on - strand, within GFF1268 at 92.520 kb on - strand, within GFF1268 at 92.549 kb on + strand, within GFF1268 at 92.549 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.761 kb on + strand, within GFF1268 at 92.761 kb on + strand, within GFF1268 at 92.762 kb on - strand, within GFF1268 at 92.762 kb on - strand, within GFF1268 at 92.794 kb on + strand, within GFF1268 at 92.795 kb on - strand, within GFF1268 at 92.795 kb on - strand, within GFF1268 at 92.819 kb on - strand at 92.872 kb on + strand at 92.906 kb on - strand at 92.906 kb on - strand at 92.910 kb on - strand at 93.042 kb on + strand, within GFF1269 at 93.042 kb on + strand, within GFF1269 at 93.101 kb on - strand, within GFF1269 at 93.101 kb on - strand, within GFF1269 at 93.126 kb on + strand, within GFF1269 at 93.126 kb on + strand, within GFF1269 at 93.126 kb on + strand, within GFF1269 at 93.127 kb on - strand, within GFF1269 at 93.127 kb on - strand, within GFF1269 at 93.127 kb on - strand, within GFF1269 at 93.198 kb on + strand, within GFF1269 at 93.335 kb on + strand at 93.335 kb on + strand at 93.335 kb on + strand at 93.335 kb on + strand at 93.345 kb on + strand at 93.348 kb on + strand at 93.348 kb on + strand at 93.537 kb on + strand at 93.537 kb on + strand at 93.537 kb on + strand at 93.538 kb on - strand at 93.543 kb on + strand at 93.786 kb on - strand, within GFF1270 at 93.786 kb on - strand, within GFF1270
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 91,562 - GFF1266 0.55 -0.7 91,652 + GFF1266 0.66 +0.5 91,652 + GFF1266 0.66 +0.6 91,663 + GFF1266 0.68 -0.2 91,663 + GFF1266 0.68 +0.4 91,722 + GFF1266 0.75 +0.5 91,911 + +0.2 91,912 - +0.6 91,933 - GFF1267 0.19 +0.6 92,161 - +0.6 92,386 - +1.1 92,404 + -0.4 92,405 - +1.0 92,405 - -0.5 92,405 - +0.5 92,437 + +0.9 92,440 + +0.6 92,512 - GFF1268 0.25 -0.7 92,512 - GFF1268 0.25 -0.2 92,517 + GFF1268 0.26 +0.9 92,517 + GFF1268 0.26 -0.1 92,517 + GFF1268 0.26 +0.5 92,517 + GFF1268 0.26 -0.4 92,517 + GFF1268 0.26 +0.3 92,517 + GFF1268 0.26 +0.2 92,518 - GFF1268 0.26 +1.1 92,518 - GFF1268 0.26 -0.4 92,518 - GFF1268 0.26 +0.3 92,518 - GFF1268 0.26 +0.7 92,518 - GFF1268 0.26 +1.0 92,520 - GFF1268 0.26 -0.1 92,520 - GFF1268 0.26 +0.2 92,520 - GFF1268 0.26 +1.5 92,549 + GFF1268 0.33 +1.1 92,549 + GFF1268 0.33 +0.2 92,759 + GFF1268 0.81 -0.4 92,759 + GFF1268 0.81 +0.3 92,759 + GFF1268 0.81 -1.6 92,759 + GFF1268 0.81 -3.0 92,759 + GFF1268 0.81 +1.6 92,761 + GFF1268 0.82 +0.2 92,761 + GFF1268 0.82 -0.4 92,762 - GFF1268 0.82 +1.2 92,762 - GFF1268 0.82 -0.6 92,794 + GFF1268 0.89 +0.1 92,795 - GFF1268 0.90 +0.2 92,795 - GFF1268 0.90 -0.3 92,819 - +0.5 92,872 + -0.2 92,906 - -0.4 92,906 - +0.5 92,910 - -0.0 93,042 + GFF1269 0.31 -0.3 93,042 + GFF1269 0.31 +0.5 93,101 - GFF1269 0.45 +0.3 93,101 - GFF1269 0.45 -1.0 93,126 + GFF1269 0.51 +0.5 93,126 + GFF1269 0.51 -0.7 93,126 + GFF1269 0.51 -0.2 93,127 - GFF1269 0.51 -1.0 93,127 - GFF1269 0.51 -0.6 93,127 - GFF1269 0.51 +0.1 93,198 + GFF1269 0.67 -0.5 93,335 + -0.2 93,335 + -0.4 93,335 + +0.0 93,335 + -1.0 93,345 + -0.4 93,348 + +0.0 93,348 + +1.0 93,537 + -0.5 93,537 + -0.4 93,537 + -0.8 93,538 - -0.1 93,543 + +0.1 93,786 - GFF1270 0.65 -0.4 93,786 - GFF1270 0.65 -0.6
Or see this region's nucleotide sequence