Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1175 and GFF1176 are separated by 19 nucleotides GFF1176 and GFF1177 are separated by 8 nucleotides GFF1177 and GFF1178 overlap by 32 nucleotides GFF1178 and GFF1179 are separated by 113 nucleotides GFF1179 and GFF1180 overlap by 14 nucleotides GFF1180 and GFF1181 overlap by 11 nucleotides
GFF1175 - IncF plasmid conjugative transfer protein TraN, at 20,907 to 22,733
GFF1175
GFF1176 - IncF plasmid conjugative transfer protein TrbE, at 22,753 to 22,959
GFF1176
GFF1177 - IncF plasmid conjugative transfer pilus assembly protein TraF, at 22,968 to 23,720
GFF1177
GFF1178 - hypothetical protein, at 23,689 to 23,820
GFF1178
GFF1179 - IncF plasmid conjugative transfer protein TraQ, at 23,934 to 24,227
GFF1179
GFF1180 - IncF plasmid conjugative transfer protein TrbB, at 24,214 to 24,762
GFF1180
GFF1181 - IncF plasmid conjugative transfer pilus assembly protein TraH, at 24,752 to 26,134
GFF1181
Position (kb)
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 22.731 kb on + strand at 22.732 kb on - strand at 22.751 kb on + strand at 22.751 kb on + strand at 22.752 kb on - strand at 22.786 kb on - strand, within GFF1176 at 22.786 kb on - strand, within GFF1176 at 22.826 kb on + strand, within GFF1176 at 22.870 kb on - strand, within GFF1176 at 22.870 kb on - strand, within GFF1176 at 22.879 kb on + strand, within GFF1176 at 22.879 kb on + strand, within GFF1176 at 22.880 kb on - strand, within GFF1176 at 22.880 kb on - strand, within GFF1176 at 22.944 kb on + strand at 22.967 kb on + strand at 22.967 kb on + strand at 22.968 kb on - strand at 22.968 kb on - strand at 23.005 kb on + strand at 23.055 kb on + strand, within GFF1177 at 23.056 kb on - strand, within GFF1177 at 23.097 kb on - strand, within GFF1177 at 23.130 kb on - strand, within GFF1177 at 23.130 kb on - strand, within GFF1177 at 23.217 kb on + strand, within GFF1177 at 23.307 kb on + strand, within GFF1177 at 23.307 kb on + strand, within GFF1177 at 23.308 kb on - strand, within GFF1177 at 23.308 kb on - strand, within GFF1177 at 23.319 kb on + strand, within GFF1177 at 23.329 kb on - strand, within GFF1177 at 23.341 kb on + strand, within GFF1177 at 23.342 kb on - strand, within GFF1177 at 23.406 kb on + strand, within GFF1177 at 23.407 kb on - strand, within GFF1177 at 23.412 kb on - strand, within GFF1177 at 23.425 kb on - strand, within GFF1177 at 23.468 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.487 kb on + strand, within GFF1177 at 23.612 kb on - strand, within GFF1177 at 23.655 kb on - strand at 23.739 kb on + strand, within GFF1178 at 23.739 kb on + strand, within GFF1178 at 23.905 kb on - strand at 23.907 kb on - strand at 24.226 kb on - strand at 24.303 kb on + strand, within GFF1180 at 24.489 kb on - strand, within GFF1180 at 24.512 kb on - strand, within GFF1180 at 24.534 kb on + strand, within GFF1180 at 24.671 kb on + strand, within GFF1180 at 24.672 kb on - strand, within GFF1180 at 24.739 kb on + strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.760 kb on + strand at 24.772 kb on + strand at 24.772 kb on + strand at 24.786 kb on + strand at 24.787 kb on - strand at 24.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 22,731 + -0.8 22,732 - -0.8 22,751 + +0.5 22,751 + -0.5 22,752 - -0.1 22,786 - GFF1176 0.16 -0.8 22,786 - GFF1176 0.16 +0.1 22,826 + GFF1176 0.35 -0.4 22,870 - GFF1176 0.57 +1.2 22,870 - GFF1176 0.57 +0.3 22,879 + GFF1176 0.61 +0.0 22,879 + GFF1176 0.61 -0.9 22,880 - GFF1176 0.61 +0.3 22,880 - GFF1176 0.61 +0.2 22,944 + -0.8 22,967 + -1.3 22,967 + +0.9 22,968 - -0.2 22,968 - -0.4 23,005 + -0.4 23,055 + GFF1177 0.12 -0.1 23,056 - GFF1177 0.12 -0.5 23,097 - GFF1177 0.17 +0.8 23,130 - GFF1177 0.22 -0.0 23,130 - GFF1177 0.22 -0.0 23,217 + GFF1177 0.33 -1.1 23,307 + GFF1177 0.45 -0.1 23,307 + GFF1177 0.45 -0.0 23,308 - GFF1177 0.45 -0.3 23,308 - GFF1177 0.45 -0.3 23,319 + GFF1177 0.47 -0.0 23,329 - GFF1177 0.48 -0.1 23,341 + GFF1177 0.50 -0.5 23,342 - GFF1177 0.50 -1.1 23,406 + GFF1177 0.58 -0.3 23,407 - GFF1177 0.58 +0.0 23,412 - GFF1177 0.59 +0.7 23,425 - GFF1177 0.61 -0.2 23,468 + GFF1177 0.66 +0.7 23,487 + GFF1177 0.69 -1.2 23,487 + GFF1177 0.69 -0.8 23,487 + GFF1177 0.69 -1.0 23,612 - GFF1177 0.86 -0.5 23,655 - -1.8 23,739 + GFF1178 0.38 +0.2 23,739 + GFF1178 0.38 -1.2 23,905 - -0.3 23,907 - -0.2 24,226 - +0.7 24,303 + GFF1180 0.16 -0.3 24,489 - GFF1180 0.50 +1.6 24,512 - GFF1180 0.54 -0.3 24,534 + GFF1180 0.58 +0.2 24,671 + GFF1180 0.83 -0.4 24,672 - GFF1180 0.83 -0.5 24,739 + +0.0 24,740 - +0.2 24,740 - +2.2 24,740 - +0.1 24,740 - +0.3 24,740 - -0.6 24,760 + +0.3 24,772 + +1.0 24,772 + -0.3 24,786 + -0.2 24,787 - -1.5 24,789 + -0.5
Or see this region's nucleotide sequence